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Evaluation of a new chromogenic medium for direct enumeration of Campylobacter in poultry meat samples.
J Food Prot. 2014 Dec; 77(12):2111-4.JF

Abstract

The present study was conducted to compare Campylobacter counts obtained by three selective media: modified charcoal cefoperazonedeoxycholate agar (mCCDA), Campy Food agar (CFA), and a novel agar RAPID' Campylobacter agar. Analysis of 12 artificially and 36 naturally contaminated samples indicated no significant differences in Campylobacter counts obtained with all three selective media. Lin's concordance correlation coefficient (CCC) and the Bland-Altman plot revealed a high level of agreement between Campylobacter counts when evaluating RAPID versus mCCDA and CFA plates. RAPID agar was the only medium tested that could effectively suppress the growth of the background microflora with naturally contaminated samples. Results of this study clearly indicated that RAPID agar is highly selective without loss of sensitivity for recovering Campylobacter. Results obtained are in agreement with those for other commonly used media; therefore, RAPID medium is suitable for Campylobacter enumeration in poultry meat samples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Seliwiorstow T
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. tomasz.seliwiorstow@ugent.be.
Baré J
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Verhaegen B
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Uyttendaele M
Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
de Zutter L
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.

MeSH

AgarAnimalsCampylobacterColony Count, MicrobialCulture MediaFood ContaminationFood MicrobiologyMeatPoultry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25474058

Citation

Seliwiorstow, Tomasz, et al. "Evaluation of a New Chromogenic Medium for Direct Enumeration of Campylobacter in Poultry Meat Samples." Journal of Food Protection, vol. 77, no. 12, 2014, pp. 2111-4.
Seliwiorstow T, Baré J, Verhaegen B, et al. Evaluation of a new chromogenic medium for direct enumeration of Campylobacter in poultry meat samples. J Food Prot. 2014;77(12):2111-4.
Seliwiorstow, T., Baré, J., Verhaegen, B., Uyttendaele, M., & de Zutter, L. (2014). Evaluation of a new chromogenic medium for direct enumeration of Campylobacter in poultry meat samples. Journal of Food Protection, 77(12), 2111-4. https://doi.org/10.4315/0362-028X.JFP-14-237
Seliwiorstow T, et al. Evaluation of a New Chromogenic Medium for Direct Enumeration of Campylobacter in Poultry Meat Samples. J Food Prot. 2014;77(12):2111-4. PubMed PMID: 25474058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a new chromogenic medium for direct enumeration of Campylobacter in poultry meat samples. AU - Seliwiorstow,Tomasz, AU - Baré,Julie, AU - Verhaegen,Bavo, AU - Uyttendaele,Mieke, AU - de Zutter,Lieven, PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2015/8/14/medline SP - 2111 EP - 4 JF - Journal of food protection JO - J Food Prot VL - 77 IS - 12 N2 - The present study was conducted to compare Campylobacter counts obtained by three selective media: modified charcoal cefoperazonedeoxycholate agar (mCCDA), Campy Food agar (CFA), and a novel agar RAPID' Campylobacter agar. Analysis of 12 artificially and 36 naturally contaminated samples indicated no significant differences in Campylobacter counts obtained with all three selective media. Lin's concordance correlation coefficient (CCC) and the Bland-Altman plot revealed a high level of agreement between Campylobacter counts when evaluating RAPID versus mCCDA and CFA plates. RAPID agar was the only medium tested that could effectively suppress the growth of the background microflora with naturally contaminated samples. Results of this study clearly indicated that RAPID agar is highly selective without loss of sensitivity for recovering Campylobacter. Results obtained are in agreement with those for other commonly used media; therefore, RAPID medium is suitable for Campylobacter enumeration in poultry meat samples. SN - 1944-9097 UR - https://www.unboundmedicine.com/medline/citation/25474058/Evaluation_of_a_new_chromogenic_medium_for_direct_enumeration_of_Campylobacter_in_poultry_meat_samples_ DB - PRIME DP - Unbound Medicine ER -