Tags

Type your tag names separated by a space and hit enter

Altered fatty acid metabolism-related gene expression in liver from morbidly obese women with non-alcoholic fatty liver disease.
Int J Mol Sci 2014; 15(12):22173-87IJ

Abstract

Lipid accumulation in the human liver seems to be a crucial mechanism in the pathogenesis and the progression of non-alcoholic fatty liver disease (NAFLD). We aimed to evaluate gene expression of different fatty acid (FA) metabolism-related genes in morbidly obese (MO) women with NAFLD. Liver expression of key genes related to de novo FA synthesis (LXRα, SREBP1c, ACC1, FAS), FA uptake and transport (PPARγ, CD36, FABP4), FA oxidation (PPARα), and inflammation (IL6, TNFα, CRP, PPARδ) were assessed by RT-qPCR in 127 MO women with normal liver histology (NL, n = 13), simple steatosis (SS, n = 47) and non-alcoholic steatohepatitis (NASH, n = 67). Liver FAS mRNA expression was significantly higher in MO NAFLD women with both SS and NASH compared to those with NL (p = 0.003, p = 0.010, respectively). Hepatic IL6 and TNFα mRNA expression was higher in NASH than in SS subjects (p = 0.033, p = 0.050, respectively). Interestingly, LXRα, ACC1 and FAS expression had an inverse relation with the grade of steatosis. These results were confirmed by western blot analysis. In conclusion, our results indicate that lipogenesis seems to be downregulated in advanced stages of SS, suggesting that, in this type of extreme obesity, the deregulation of the lipogenic pathway might be associated with the severity of steatosis.

Authors+Show Affiliations

Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain. aporras.hj23.ics@gencat.cat.Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain. alba.berlanga@urv.cat.Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain.Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, Tarragona 43007, Spain. mgonzalez.hj23.ics@gencat.cat.Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, Tarragona 43007, Spain. aporras.hj23.ics@gencat.cat.Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain. gemma.aragones@iispv.cat.Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, Tarragona 43204, Spain. fatima.sabench@urv.cat.Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, Tarragona 43204, Spain. mhernandezg@grupsagessa.com.Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain. caguilar.hj23.ics@gencat.cat.Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, Tarragona 43007, Spain. jsirvent.hj23.ics@gencat.cat.Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, Tarragona 43204, Spain. ddelcastillo@grupsagessa.com.Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Sanitària Pere Virgili IISPV (IISPV), Tarragona 43003, Spain. crichart.hj23.ics@gencat.cat.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25474087

Citation

Auguet, Teresa, et al. "Altered Fatty Acid Metabolism-related Gene Expression in Liver From Morbidly Obese Women With Non-alcoholic Fatty Liver Disease." International Journal of Molecular Sciences, vol. 15, no. 12, 2014, pp. 22173-87.
Auguet T, Berlanga A, Guiu-Jurado E, et al. Altered fatty acid metabolism-related gene expression in liver from morbidly obese women with non-alcoholic fatty liver disease. Int J Mol Sci. 2014;15(12):22173-87.
Auguet, T., Berlanga, A., Guiu-Jurado, E., Martinez, S., Porras, J. A., Aragonès, G., ... Richart, C. (2014). Altered fatty acid metabolism-related gene expression in liver from morbidly obese women with non-alcoholic fatty liver disease. International Journal of Molecular Sciences, 15(12), pp. 22173-87. doi:10.3390/ijms151222173.
Auguet T, et al. Altered Fatty Acid Metabolism-related Gene Expression in Liver From Morbidly Obese Women With Non-alcoholic Fatty Liver Disease. Int J Mol Sci. 2014 Dec 2;15(12):22173-87. PubMed PMID: 25474087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered fatty acid metabolism-related gene expression in liver from morbidly obese women with non-alcoholic fatty liver disease. AU - Auguet,Teresa, AU - Berlanga,Alba, AU - Guiu-Jurado,Esther, AU - Martinez,Salomé, AU - Porras,José Antonio, AU - Aragonès,Gemma, AU - Sabench,Fátima, AU - Hernandez,Mercé, AU - Aguilar,Carmen, AU - Sirvent,Joan Josep, AU - Del Castillo,Daniel, AU - Richart,Cristóbal, Y1 - 2014/12/02/ PY - 2014/10/02/received PY - 2014/11/25/revised PY - 2014/11/25/accepted PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2015/7/17/medline SP - 22173 EP - 87 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 15 IS - 12 N2 - Lipid accumulation in the human liver seems to be a crucial mechanism in the pathogenesis and the progression of non-alcoholic fatty liver disease (NAFLD). We aimed to evaluate gene expression of different fatty acid (FA) metabolism-related genes in morbidly obese (MO) women with NAFLD. Liver expression of key genes related to de novo FA synthesis (LXRα, SREBP1c, ACC1, FAS), FA uptake and transport (PPARγ, CD36, FABP4), FA oxidation (PPARα), and inflammation (IL6, TNFα, CRP, PPARδ) were assessed by RT-qPCR in 127 MO women with normal liver histology (NL, n = 13), simple steatosis (SS, n = 47) and non-alcoholic steatohepatitis (NASH, n = 67). Liver FAS mRNA expression was significantly higher in MO NAFLD women with both SS and NASH compared to those with NL (p = 0.003, p = 0.010, respectively). Hepatic IL6 and TNFα mRNA expression was higher in NASH than in SS subjects (p = 0.033, p = 0.050, respectively). Interestingly, LXRα, ACC1 and FAS expression had an inverse relation with the grade of steatosis. These results were confirmed by western blot analysis. In conclusion, our results indicate that lipogenesis seems to be downregulated in advanced stages of SS, suggesting that, in this type of extreme obesity, the deregulation of the lipogenic pathway might be associated with the severity of steatosis. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/25474087/Altered_fatty_acid_metabolism_related_gene_expression_in_liver_from_morbidly_obese_women_with_non_alcoholic_fatty_liver_disease_ L2 - http://www.mdpi.com/resolver?pii=ijms151222173 DB - PRIME DP - Unbound Medicine ER -