Tags

Type your tag names separated by a space and hit enter

Differential expression of basal microRNAs' patterns in human dental pulp stem cells.
J Cell Mol Med. 2015 Mar; 19(3):566-80.JC

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate translation of mRNA into protein and play a crucial role for almost all biological activities. However, the identification of miRNAs from mesenchymal stem cells (MSCs), especially from dental pulp, is poorly understood. In this study, dental pulp stem cells (DPSCs) were characterized in terms of their proliferation and differentiation capacity. Furthermore, 104 known mature miRNAs were profiled by using real-time PCR. Notably, we observed 19 up-regulated miRNAs and 29 significantly down-regulated miRNAs in DPSCs in comparison with bone marrow MSCs (BM-MSCs). The 19 up-regulated miRNAs were subjected to ingenuity analysis, which were composed into 25 functional networks. We have chosen top 2 functional networks, which comprised 10 miRNA (hsa-miR-516a-3p, hsa-miR-125b-1-3p, hsa-miR-221-5p, hsa-miR-7, hsa-miR-584-5p, hsa-miR-190a, hsa-miR-106a-5p, hsa-mir-376a-5p, hsa-mir-377-5p and hsa-let-7f-2-3p). Prediction of target mRNAs and associated biological pathways regulated by each of this miRNA was carried out. We paid special attention to hsa-miR-516a-3p and hsa-miR-7-5p as these miRNAs were highly expressed upon validation with qRT-PCR analysis. We further proceeded with loss-of-function analysis with these miRNAs and we observed that hsa-miR-516a-3p knockdown induced a significant increase in the expression of WNT5A. Likewise, the knockdown of hsa-miR-7-5p increased the expression of EGFR. Nevertheless, further validation revealed the role of WNT5A as an indirect target of hsa-miR-516a-3p. These results provide new insights into the dynamic role of miRNA expression in DPSCs. In conclusion, using miRNA signatures in human as a prediction tool will enable us to elucidate the biological processes occurring in DPSCs.

Links

PMC Free PDF

Authors+Show Affiliations

Vasanthan P
Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Govindasamy V
No affiliation info available
Gnanasegaran N
No affiliation info available
Kunasekaran W
No affiliation info available
Musa S
No affiliation info available
Abu Kasim NH
No affiliation info available

MeSH

Bone Marrow CellsCell DifferentiationCell ProliferationDental PulpGene Expression ProfilingGene Expression RegulationGene Knockdown TechniquesHumansMesenchymal Stem CellsMicroRNAsOligonucleotide Array Sequence AnalysisProto-Oncogene ProteinsReal-Time Polymerase Chain ReactionSignal TransductionWnt ProteinsWnt-5a Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25475098

Citation

Vasanthan, Punitha, et al. "Differential Expression of Basal microRNAs' Patterns in Human Dental Pulp Stem Cells." Journal of Cellular and Molecular Medicine, vol. 19, no. 3, 2015, pp. 566-80.
Vasanthan P, Govindasamy V, Gnanasegaran N, et al. Differential expression of basal microRNAs' patterns in human dental pulp stem cells. J Cell Mol Med. 2015;19(3):566-80.
Vasanthan, P., Govindasamy, V., Gnanasegaran, N., Kunasekaran, W., Musa, S., & Abu Kasim, N. H. (2015). Differential expression of basal microRNAs' patterns in human dental pulp stem cells. Journal of Cellular and Molecular Medicine, 19(3), 566-80. https://doi.org/10.1111/jcmm.12381
Vasanthan P, et al. Differential Expression of Basal microRNAs' Patterns in Human Dental Pulp Stem Cells. J Cell Mol Med. 2015;19(3):566-80. PubMed PMID: 25475098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of basal microRNAs' patterns in human dental pulp stem cells. AU - Vasanthan,Punitha, AU - Govindasamy,Vijayendran, AU - Gnanasegaran,Nareshwaran, AU - Kunasekaran,Wijenthiran, AU - Musa,Sabri, AU - Abu Kasim,Noor Hayaty, Y1 - 2014/12/05/ PY - 2014/02/27/received PY - 2014/07/02/accepted PY - 2014/12/6/entrez PY - 2014/12/6/pubmed PY - 2015/9/12/medline KW - gene expression KW - medical biotechnology KW - mesenchymal stem cells KW - signalling network SP - 566 EP - 80 JF - Journal of cellular and molecular medicine JO - J Cell Mol Med VL - 19 IS - 3 N2 - MicroRNAs (miRNAs) are small non-coding RNAs that regulate translation of mRNA into protein and play a crucial role for almost all biological activities. However, the identification of miRNAs from mesenchymal stem cells (MSCs), especially from dental pulp, is poorly understood. In this study, dental pulp stem cells (DPSCs) were characterized in terms of their proliferation and differentiation capacity. Furthermore, 104 known mature miRNAs were profiled by using real-time PCR. Notably, we observed 19 up-regulated miRNAs and 29 significantly down-regulated miRNAs in DPSCs in comparison with bone marrow MSCs (BM-MSCs). The 19 up-regulated miRNAs were subjected to ingenuity analysis, which were composed into 25 functional networks. We have chosen top 2 functional networks, which comprised 10 miRNA (hsa-miR-516a-3p, hsa-miR-125b-1-3p, hsa-miR-221-5p, hsa-miR-7, hsa-miR-584-5p, hsa-miR-190a, hsa-miR-106a-5p, hsa-mir-376a-5p, hsa-mir-377-5p and hsa-let-7f-2-3p). Prediction of target mRNAs and associated biological pathways regulated by each of this miRNA was carried out. We paid special attention to hsa-miR-516a-3p and hsa-miR-7-5p as these miRNAs were highly expressed upon validation with qRT-PCR analysis. We further proceeded with loss-of-function analysis with these miRNAs and we observed that hsa-miR-516a-3p knockdown induced a significant increase in the expression of WNT5A. Likewise, the knockdown of hsa-miR-7-5p increased the expression of EGFR. Nevertheless, further validation revealed the role of WNT5A as an indirect target of hsa-miR-516a-3p. These results provide new insights into the dynamic role of miRNA expression in DPSCs. In conclusion, using miRNA signatures in human as a prediction tool will enable us to elucidate the biological processes occurring in DPSCs. SN - 1582-4934 UR - https://www.unboundmedicine.com/medline/citation/25475098/Differential_expression_of_basal_microRNAs'_patterns_in_human_dental_pulp_stem_cells_ DB - PRIME DP - Unbound Medicine ER -