Tags

Type your tag names separated by a space and hit enter

Study on aggregation behavior of Cytochrome C-conjugated silver nanoparticles using asymmetrical flow field-flow fractionation.
Talanta. 2015 Jan; 132:939-44.T

Abstract

In this study, 40 nm silver nanoparticles (AgNPs) were synthesized using the citrate reduction method and then the surface of AgNPs was modified by conjugating Cytochrome C (Cyto C) to improve stability and to enhance bioactivity and biocompatibility of AgNPs. It is known that Cyto C may undergo conformational changes under various conditions of pH, temperature, ionic strength, etc., resulting in aggregation of the particles. These parameters also affect the size and size distribution of Cyto C-conjugated AgNPs (Cyto C-AgNP). ζ-potential measurement revealed that the adsorption of Cyto C on the surface of AgNPs is saturated at the molar ratio [Cyto C]/[AgNPs] above about 300. Asymmetrical flow field-flow fractionation (AsFlFFF) analysis showed that hydrodynamic diameter of AgNPs increases by about 4 nm when the particle is saturated by Cyto C. The aggregation behavior of Cyto C-AgNP at various conditions of pH, temperature and ionic strength were investigated using AsFlFFF and UV-vis spectroscopy. It was found that the aggregation of Cyto C-AgNP increases with decreasing pH, increasing temperature and ionic strength due to denaturation of Cyto C on AgNPs and reduction in the thickness of electrostatic double layer on the surface of Cyto C-AgNP.

Authors+Show Affiliations

Department of Chemistry, Hannam University, Daejeon 305-811, South Korea.Gyungnam Department of Environmental Toxicology, Korea Institute of Toxicology, Jinju 660-844, South Korea.Gyungnam Department of Environmental Toxicology, Korea Institute of Toxicology, Jinju 660-844, South Korea.Department of Chemistry, Hannam University, Daejeon 305-811, South Korea. Electronic address: slee@hannam.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25476400

Citation

Kim, Sun Tae, et al. "Study On Aggregation Behavior of Cytochrome C-conjugated Silver Nanoparticles Using Asymmetrical Flow Field-flow Fractionation." Talanta, vol. 132, 2015, pp. 939-44.
Kim ST, Lee YJ, Hwang YS, et al. Study on aggregation behavior of Cytochrome C-conjugated silver nanoparticles using asymmetrical flow field-flow fractionation. Talanta. 2015;132:939-44.
Kim, S. T., Lee, Y. J., Hwang, Y. S., & Lee, S. (2015). Study on aggregation behavior of Cytochrome C-conjugated silver nanoparticles using asymmetrical flow field-flow fractionation. Talanta, 132, 939-44. https://doi.org/10.1016/j.talanta.2014.05.060
Kim ST, et al. Study On Aggregation Behavior of Cytochrome C-conjugated Silver Nanoparticles Using Asymmetrical Flow Field-flow Fractionation. Talanta. 2015;132:939-44. PubMed PMID: 25476400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study on aggregation behavior of Cytochrome C-conjugated silver nanoparticles using asymmetrical flow field-flow fractionation. AU - Kim,Sun Tae, AU - Lee,Yong-Ju, AU - Hwang,Yu-Sik, AU - Lee,Seungho, Y1 - 2014/06/10/ PY - 2014/04/22/received PY - 2014/05/16/revised PY - 2014/05/20/accepted PY - 2014/12/6/entrez PY - 2014/12/6/pubmed PY - 2015/8/8/medline KW - Aggregation KW - Asymmetrical flow field-flow fractionation (AsFlFFF) KW - Cytochrome C–conjugated AgNPs KW - UV–vis spectroscopy KW - ζ-potential SP - 939 EP - 44 JF - Talanta JO - Talanta VL - 132 N2 - In this study, 40 nm silver nanoparticles (AgNPs) were synthesized using the citrate reduction method and then the surface of AgNPs was modified by conjugating Cytochrome C (Cyto C) to improve stability and to enhance bioactivity and biocompatibility of AgNPs. It is known that Cyto C may undergo conformational changes under various conditions of pH, temperature, ionic strength, etc., resulting in aggregation of the particles. These parameters also affect the size and size distribution of Cyto C-conjugated AgNPs (Cyto C-AgNP). ζ-potential measurement revealed that the adsorption of Cyto C on the surface of AgNPs is saturated at the molar ratio [Cyto C]/[AgNPs] above about 300. Asymmetrical flow field-flow fractionation (AsFlFFF) analysis showed that hydrodynamic diameter of AgNPs increases by about 4 nm when the particle is saturated by Cyto C. The aggregation behavior of Cyto C-AgNP at various conditions of pH, temperature and ionic strength were investigated using AsFlFFF and UV-vis spectroscopy. It was found that the aggregation of Cyto C-AgNP increases with decreasing pH, increasing temperature and ionic strength due to denaturation of Cyto C on AgNPs and reduction in the thickness of electrostatic double layer on the surface of Cyto C-AgNP. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25476400/Study_on_aggregation_behavior_of_Cytochrome_C_conjugated_silver_nanoparticles_using_asymmetrical_flow_field_flow_fractionation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(14)00456-1 DB - PRIME DP - Unbound Medicine ER -