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Investigating the antifungal activity and mechanism(s) of geraniol against Candida albicans strains.
Med Mycol. 2015 Apr; 53(3):275-84.MM

Abstract

Candida albicans can be a yeast that is a commensal on the human body but can cause opportunistic or pathogenic infections. Candida infections may create serious health problems and as a result has initiated a search for new drugs with an antifungal action. Geraniol is an acyclic monoterpene alcohol with known pharmacological properties, including antimicrobial activity. The aim of this work was to evaluate the antifungal activity and mechanism(s) of geraniol against C. albicans strains. The minimum inhibitory concentration (MIC) was determined through broth microdilution techniques. We investigated possible geraniol activity on the fungal cell wall (sorbitol protect effect), cell membrane (geraniol to ergosterol binding), the time-kill curve, and its biological activity on the yeast's morphology. Amphotericin B was used as control, and all tests were performed in duplicate. The MIC of geraniol was 16 μg/ml (for 90% of isolates) but its probable mechanism of action did not involve the cell wall and ergosterol binding. In the morphological interference assay, we observed that the product inhibited pseudohyphae and chlamydoconidia formation. Time-dependent kill curve assay demonstrated that the fungicidal activity for MIC × 2 started at 2 h for the ATCC 76485 strain, and at 4 h for the LM-70 strain. Geraniol showed in vitro antifungal potential against strains of C. albicans but did not involve action on the cell wall or ergosterol. This study contributes to the development of new antifungal drugs, especially against Candida spp.

Authors+Show Affiliations

Federal Institute of Education, Science, and Technology of Paraíba (IFPB), Itaporanga, Paraíba, Brazil Mycology Laboratory, Departament of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, 58051-970, Brazil cleryaalvino@hotmail.com.Mycology Laboratory, Departament of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, 58051-970, Brazil.Mycology Laboratory, Departament of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, 58051-970, Brazil.Mycology Laboratory, Departament of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, 58051-970, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25480017

Citation

Leite, Maria Clerya Alvino, et al. "Investigating the Antifungal Activity and Mechanism(s) of Geraniol Against Candida Albicans Strains." Medical Mycology, vol. 53, no. 3, 2015, pp. 275-84.
Leite MC, de Brito Bezerra AP, de Sousa JP, et al. Investigating the antifungal activity and mechanism(s) of geraniol against Candida albicans strains. Med Mycol. 2015;53(3):275-84.
Leite, M. C., de Brito Bezerra, A. P., de Sousa, J. P., & de Oliveira Lima, E. (2015). Investigating the antifungal activity and mechanism(s) of geraniol against Candida albicans strains. Medical Mycology, 53(3), 275-84. https://doi.org/10.1093/mmy/myu078
Leite MC, et al. Investigating the Antifungal Activity and Mechanism(s) of Geraniol Against Candida Albicans Strains. Med Mycol. 2015;53(3):275-84. PubMed PMID: 25480017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the antifungal activity and mechanism(s) of geraniol against Candida albicans strains. AU - Leite,Maria Clerya Alvino, AU - de Brito Bezerra,André Parente, AU - de Sousa,Janiere Pereira, AU - de Oliveira Lima,Edeltrudes, Y1 - 2014/12/05/ PY - 2014/12/7/entrez PY - 2014/12/7/pubmed PY - 2016/2/9/medline KW - Candida albicans KW - antifungal activity KW - biological products KW - geraniol KW - terpenes SP - 275 EP - 84 JF - Medical mycology JO - Med Mycol VL - 53 IS - 3 N2 - Candida albicans can be a yeast that is a commensal on the human body but can cause opportunistic or pathogenic infections. Candida infections may create serious health problems and as a result has initiated a search for new drugs with an antifungal action. Geraniol is an acyclic monoterpene alcohol with known pharmacological properties, including antimicrobial activity. The aim of this work was to evaluate the antifungal activity and mechanism(s) of geraniol against C. albicans strains. The minimum inhibitory concentration (MIC) was determined through broth microdilution techniques. We investigated possible geraniol activity on the fungal cell wall (sorbitol protect effect), cell membrane (geraniol to ergosterol binding), the time-kill curve, and its biological activity on the yeast's morphology. Amphotericin B was used as control, and all tests were performed in duplicate. The MIC of geraniol was 16 μg/ml (for 90% of isolates) but its probable mechanism of action did not involve the cell wall and ergosterol binding. In the morphological interference assay, we observed that the product inhibited pseudohyphae and chlamydoconidia formation. Time-dependent kill curve assay demonstrated that the fungicidal activity for MIC × 2 started at 2 h for the ATCC 76485 strain, and at 4 h for the LM-70 strain. Geraniol showed in vitro antifungal potential against strains of C. albicans but did not involve action on the cell wall or ergosterol. This study contributes to the development of new antifungal drugs, especially against Candida spp. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/25480017/Investigating_the_antifungal_activity_and_mechanism_s__of_geraniol_against_Candida_albicans_strains_ DB - PRIME DP - Unbound Medicine ER -