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Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.).
BMC Genet. 2014 Dec 10; 15:133.BG

Abstract

BACKGROUND

Peanut is one of the major source for human consumption worldwide and its seed contain approximately 50% oil. Improvement of oil content and quality traits (high oleic and low linoleic acid) in peanut could be accelerated by exploiting linked markers through molecular breeding. The objective of this study was to identify QTLs associated with oil content, and estimate relative contribution of FAD2 genes (ahFAD2A and ahFAD2B) to oil quality traits in two recombinant inbred line (RIL) populations.

RESULTS

Improved genetic linkage maps were developed for S-population (SunOleic 97R × NC94022) with 206 (1780.6 cM) and T-population (Tifrunner × GT-C20) with 378 (2487.4 cM) marker loci. A total of 6 and 9 QTLs controlling oil content were identified in the S- and T-population, respectively. The contribution of each QTL towards oil content variation ranged from 3.07 to 10.23% in the S-population and from 3.93 to 14.07% in the T-population. The mapping positions for ahFAD2A (A sub-genome) and ahFAD2B (B sub-genome) genes were assigned on a09 and b09 linkage groups. The ahFAD2B gene (26.54%, 25.59% and 41.02% PVE) had higher phenotypic effect on oleic acid (C18:1), linoleic acid (C18:2), and oleic/linoleic acid ratio (O/L ratio) than ahFAD2A gene (8.08%, 6.86% and 3.78% PVE). The FAD2 genes had no effect on oil content. This study identified a total of 78 main-effect QTLs (M-QTLs) with up to 42.33% phenotypic variation (PVE) and 10 epistatic QTLs (E-QTLs) up to 3.31% PVE for oil content and quality traits.

CONCLUSIONS

A total of 78 main-effect QTLs (M-QTLs) and 10 E-QTLs have been detected for oil content and oil quality traits. One major QTL (more than 10% PVE) was identified in both the populations for oil content with source alleles from NC94022 and GT-C20 parental genotypes. FAD2 genes showed high effect for oleic acid (C18:1), linoleic acid (C18:2), and O/L ratio while no effect on total oil content. The information on phenotypic effect of FAD2 genes for oleic acid, linoleic acid and O/L ratio, and oil content will be applied in breeding selection.

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Authors+Show Affiliations

Pandey MK
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. m.pandey@cgiar.org. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India. m.pandey@cgiar.org. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. m.pandey@cgiar.org.
Wang ML
US Department of Agriculture-Agricultural Research Service, Plant Genetic Resources Conservation Unit, Griffin, GA, USA. mingli.wang@ars.usda.gov.
Qiao L
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. lxqiao73@163.com. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. lxqiao73@163.com. College of Life Science, Qingdao Agricultural University, Qingdao, China. lxqiao73@163.com.
Feng S
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. supingfeng1972@gmail.com. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. supingfeng1972@gmail.com. College of Bioscience and Biotechnology, Qiongzhou University, Sanya, China. supingfeng1972@gmail.com.
Khera P
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. pkhera@uga.edu. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India. pkhera@uga.edu. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. pkhera@uga.edu.
Wang H
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. huixu@uga.edu. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. huixu@uga.edu. Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, China. huixu@uga.edu.
Tonnis B
US Department of Agriculture-Agricultural Research Service, Plant Genetic Resources Conservation Unit, Griffin, GA, USA. brandon.tonnis@ars.usda.gov.
Barkley NA
US Department of Agriculture-Agricultural Research Service, Plant Genetic Resources Conservation Unit, Griffin, GA, USA. elle.barkley@ars.usda.gov.
Wang J
Department of Agronomy, University of Florida, Gainesville, FL, USA. wangjp@ufl.edu.
Holbrook CC
US Department of Agriculture-Agricultural Research Service, Crop Genetics and Breeding Research Unit, Tifton, GA, USA. corley.holbrook@ars.usda.gov.
Culbreath AK
Department of Plant Pathology, University of Georgia, Tifton, GA, USA. spotwilt@uga.edu.
Varshney RK
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India. r.k.varshney@cgiar.org.
Guo B
US Department of Agriculture-Agricultural Research Service, Crop Protection and Management Research Unit, Tifton, GA, USA. baozhu.guo@ars.usda.gov. Department of Plant Pathology, University of Georgia, Tifton, GA, USA. baozhu.guo@ars.usda.gov.

MeSH

ArachisBreedingChromosome MappingEpistasis, GeneticFatty Acid DesaturasesFood QualityGenes, PlantGenetic Association StudiesPlant OilsQuantitative Trait Loci

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25491595

Citation

Pandey, Manish K., et al. "Identification of QTLs Associated With Oil Content and Mapping FAD2 Genes and Their Relative Contribution to Oil Quality in Peanut (Arachis Hypogaea L.)." BMC Genetics, vol. 15, 2014, p. 133.
Pandey MK, Wang ML, Qiao L, et al. Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.). BMC Genet. 2014;15:133.
Pandey, M. K., Wang, M. L., Qiao, L., Feng, S., Khera, P., Wang, H., Tonnis, B., Barkley, N. A., Wang, J., Holbrook, C. C., Culbreath, A. K., Varshney, R. K., & Guo, B. (2014). Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.). BMC Genetics, 15, 133. https://doi.org/10.1186/s12863-014-0133-4
Pandey MK, et al. Identification of QTLs Associated With Oil Content and Mapping FAD2 Genes and Their Relative Contribution to Oil Quality in Peanut (Arachis Hypogaea L.). BMC Genet. 2014 Dec 10;15:133. PubMed PMID: 25491595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.). AU - Pandey,Manish K, AU - Wang,Ming Li, AU - Qiao,Lixian, AU - Feng,Suping, AU - Khera,Pawan, AU - Wang,Hui, AU - Tonnis,Brandon, AU - Barkley,Noelle A, AU - Wang,Jianping, AU - Holbrook,C Corley, AU - Culbreath,Albert K, AU - Varshney,Rajeev K, AU - Guo,Baozhu, Y1 - 2014/12/10/ PY - 2014/07/25/received PY - 2014/11/20/accepted PY - 2014/12/11/entrez PY - 2014/12/11/pubmed PY - 2015/10/3/medline SP - 133 EP - 133 JF - BMC genetics JO - BMC Genet VL - 15 N2 - BACKGROUND: Peanut is one of the major source for human consumption worldwide and its seed contain approximately 50% oil. Improvement of oil content and quality traits (high oleic and low linoleic acid) in peanut could be accelerated by exploiting linked markers through molecular breeding. The objective of this study was to identify QTLs associated with oil content, and estimate relative contribution of FAD2 genes (ahFAD2A and ahFAD2B) to oil quality traits in two recombinant inbred line (RIL) populations. RESULTS: Improved genetic linkage maps were developed for S-population (SunOleic 97R × NC94022) with 206 (1780.6 cM) and T-population (Tifrunner × GT-C20) with 378 (2487.4 cM) marker loci. A total of 6 and 9 QTLs controlling oil content were identified in the S- and T-population, respectively. The contribution of each QTL towards oil content variation ranged from 3.07 to 10.23% in the S-population and from 3.93 to 14.07% in the T-population. The mapping positions for ahFAD2A (A sub-genome) and ahFAD2B (B sub-genome) genes were assigned on a09 and b09 linkage groups. The ahFAD2B gene (26.54%, 25.59% and 41.02% PVE) had higher phenotypic effect on oleic acid (C18:1), linoleic acid (C18:2), and oleic/linoleic acid ratio (O/L ratio) than ahFAD2A gene (8.08%, 6.86% and 3.78% PVE). The FAD2 genes had no effect on oil content. This study identified a total of 78 main-effect QTLs (M-QTLs) with up to 42.33% phenotypic variation (PVE) and 10 epistatic QTLs (E-QTLs) up to 3.31% PVE for oil content and quality traits. CONCLUSIONS: A total of 78 main-effect QTLs (M-QTLs) and 10 E-QTLs have been detected for oil content and oil quality traits. One major QTL (more than 10% PVE) was identified in both the populations for oil content with source alleles from NC94022 and GT-C20 parental genotypes. FAD2 genes showed high effect for oleic acid (C18:1), linoleic acid (C18:2), and O/L ratio while no effect on total oil content. The information on phenotypic effect of FAD2 genes for oleic acid, linoleic acid and O/L ratio, and oil content will be applied in breeding selection. SN - 1471-2156 UR - https://www.unboundmedicine.com/medline/citation/25491595/Identification_of_QTLs_associated_with_oil_content_and_mapping_FAD2_genes_and_their_relative_contribution_to_oil_quality_in_peanut__Arachis_hypogaea_L___ DB - PRIME DP - Unbound Medicine ER -