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Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development.
J Ovarian Res 2014; 7:117JO

Abstract

BACKGROUND

The protein kinase C (PKC) is a family of serine/threonine kinases that consists of 12 different isoforms. Since PKC isoform expressions are known to be specific for different cell types and postnatal developmental stages, we aimed to determine immunolocalizations and protein expression levels of different PKC isoforms in pre-pubertal, pubertal and adult mouse ovaries.

METHODS

Ovaries were obtained from postnatal day 1 (PND1) and PND7 of pre-pubertal, PND21 of pubertal and PND60 of adult mice. Immunolocalizations of PKCα, PKCδ and PKCε isoforms were determined and immunostainings in different cellular components of all follicular stages were evaluated by H-Score. PKCα, PKCδ and PKCε protein expression levels were determined by Western blot. The bands were quantified via ImageJ software. The data obtained from H-Score and ImageJ evaluations were analyzed by ANOVA statistical test.

RESULTS

PKCα immunostainings were more intense in oocytes when compared to granulosa and theca cells at different follicular stages of all groups. The Western blot analysis revealed that PKCα expression was significantly higher in PND60 adult ovaries. Conversely, PKCδ immunostainings were more intense in granulosa cells. According to the Western blot analysis, PKCδ protein expression was also higher in PND60 and significantly lower in PND1 ovaries. PKCε immunostaining was more apparent in oocytes. PKCε protein expression was significantly higher in adult PND60 and pubertal PND21 ovaries when compared to pre-pubertal PND7 and PND1 ovaries. Interestingly, PKCε immunostaining was significantly higher in primordial follicles, though PKCα and PKCδ immunostainings were more apparent in larger follicles. PKCα immunostainings of corpora lutea (CL) were significantly higher when compared to follicles in PND60 ovaries.

CONCLUSIONS

This study demonstrates that PKCα, PKCδ and PKCε isoforms are differentially expressed in particular cellular components of pre-pubertal, pubertal and adult mouse ovarian follicles. Therefore, we suggest that each PKC isoform has unique functions that are controlled by gonadotropin dependent mechanisms during follicular growth, oocyte maturation, ovulation and luteinization.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25491605

Citation

Tepekoy, Filiz, et al. "Protein Kinase C Isoforms Α, Δ and Ε Are Differentially Expressed in Mouse Ovaries at Different Stages of Postnatal Development." Journal of Ovarian Research, vol. 7, 2014, p. 117.
Tepekoy F, Ustunel I, Akkoyunlu G. Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development. J Ovarian Res. 2014;7:117.
Tepekoy, F., Ustunel, I., & Akkoyunlu, G. (2014). Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development. Journal of Ovarian Research, 7, p. 117. doi:10.1186/s13048-014-0117-z.
Tepekoy F, Ustunel I, Akkoyunlu G. Protein Kinase C Isoforms Α, Δ and Ε Are Differentially Expressed in Mouse Ovaries at Different Stages of Postnatal Development. J Ovarian Res. 2014 Dec 10;7:117. PubMed PMID: 25491605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development. AU - Tepekoy,Filiz, AU - Ustunel,Ismail, AU - Akkoyunlu,Gokhan, Y1 - 2014/12/10/ PY - 2014/07/14/received PY - 2014/11/29/accepted PY - 2014/12/11/entrez PY - 2014/12/11/pubmed PY - 2015/8/15/medline SP - 117 EP - 117 JF - Journal of ovarian research JO - J Ovarian Res VL - 7 N2 - BACKGROUND: The protein kinase C (PKC) is a family of serine/threonine kinases that consists of 12 different isoforms. Since PKC isoform expressions are known to be specific for different cell types and postnatal developmental stages, we aimed to determine immunolocalizations and protein expression levels of different PKC isoforms in pre-pubertal, pubertal and adult mouse ovaries. METHODS: Ovaries were obtained from postnatal day 1 (PND1) and PND7 of pre-pubertal, PND21 of pubertal and PND60 of adult mice. Immunolocalizations of PKCα, PKCδ and PKCε isoforms were determined and immunostainings in different cellular components of all follicular stages were evaluated by H-Score. PKCα, PKCδ and PKCε protein expression levels were determined by Western blot. The bands were quantified via ImageJ software. The data obtained from H-Score and ImageJ evaluations were analyzed by ANOVA statistical test. RESULTS: PKCα immunostainings were more intense in oocytes when compared to granulosa and theca cells at different follicular stages of all groups. The Western blot analysis revealed that PKCα expression was significantly higher in PND60 adult ovaries. Conversely, PKCδ immunostainings were more intense in granulosa cells. According to the Western blot analysis, PKCδ protein expression was also higher in PND60 and significantly lower in PND1 ovaries. PKCε immunostaining was more apparent in oocytes. PKCε protein expression was significantly higher in adult PND60 and pubertal PND21 ovaries when compared to pre-pubertal PND7 and PND1 ovaries. Interestingly, PKCε immunostaining was significantly higher in primordial follicles, though PKCα and PKCδ immunostainings were more apparent in larger follicles. PKCα immunostainings of corpora lutea (CL) were significantly higher when compared to follicles in PND60 ovaries. CONCLUSIONS: This study demonstrates that PKCα, PKCδ and PKCε isoforms are differentially expressed in particular cellular components of pre-pubertal, pubertal and adult mouse ovarian follicles. Therefore, we suggest that each PKC isoform has unique functions that are controlled by gonadotropin dependent mechanisms during follicular growth, oocyte maturation, ovulation and luteinization. SN - 1757-2215 UR - https://www.unboundmedicine.com/medline/citation/25491605/Protein_kinase_C_isoforms_α_δ_and_ε_are_differentially_expressed_in_mouse_ovaries_at_different_stages_of_postnatal_development_ L2 - https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-014-0117-z DB - PRIME DP - Unbound Medicine ER -