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Nrf2-mediated HO-1 induction contributes to antioxidant capacity of a Schisandrae Fructus ethanol extract in C2C12 myoblasts.
Nutrients. 2014 Dec; 6(12):5667-78.N

Abstract

This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H2O2)-induced inhibition of growth and induced scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2. SFEE also inhibited comet tail formation and phospho-histone γH2A.X expression, suggesting that it prevents H2O2-induced cellular DNA damage. Furthermore, treating C2C12 cells with SFEE significantly induced heme oxygenase-1 (HO-1) and phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a potent inhibitor of HO-1 activity, significantly reversed the protective effects of SFEE against H2O2-induced growth inhibition and ROS generation in C2C12 cells. Additional experiments revealed that the potential of the SFEE to induce HO-1 expression and protect against H2O2-mediated cellular damage was abrogated by transient transfection with Nrf2-specific small interfering RNA, suggesting that the SFEE protected C2C12 cells against oxidative stress-induced injury through the Nrf2/HO-1 pathway.

Authors+Show Affiliations

Blue-Bio Industry Regional Innovation Center and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea. 13839@deu.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25493944

Citation

Kang, Ji Sook, et al. "Nrf2-mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts." Nutrients, vol. 6, no. 12, 2014, pp. 5667-78.
Kang JS, Han MH, Kim GY, et al. Nrf2-mediated HO-1 induction contributes to antioxidant capacity of a Schisandrae Fructus ethanol extract in C2C12 myoblasts. Nutrients. 2014;6(12):5667-78.
Kang, J. S., Han, M. H., Kim, G. Y., Kim, C. M., Kim, B. W., Hwang, H. J., & Hyun, Y. (2014). Nrf2-mediated HO-1 induction contributes to antioxidant capacity of a Schisandrae Fructus ethanol extract in C2C12 myoblasts. Nutrients, 6(12), 5667-78.
Kang JS, et al. Nrf2-mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts. Nutrients. 2014;6(12):5667-78. PubMed PMID: 25493944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nrf2-mediated HO-1 induction contributes to antioxidant capacity of a Schisandrae Fructus ethanol extract in C2C12 myoblasts. AU - Kang,Ji Sook, AU - Han,Min Ho, AU - Kim,Gi-Young, AU - Kim,Cheol Min, AU - Kim,Byung Woo, AU - Hwang,Hye Jin, AU - Hyun,Yung, PY - 2014/10/08/received PY - 2014/11/16/revised PY - 2014/11/24/accepted PY - 2014/12/11/entrez PY - 2014/12/11/pubmed PY - 2015/11/6/medline SP - 5667 EP - 78 JF - Nutrients JO - Nutrients VL - 6 IS - 12 N2 - This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H2O2)-induced inhibition of growth and induced scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2. SFEE also inhibited comet tail formation and phospho-histone γH2A.X expression, suggesting that it prevents H2O2-induced cellular DNA damage. Furthermore, treating C2C12 cells with SFEE significantly induced heme oxygenase-1 (HO-1) and phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a potent inhibitor of HO-1 activity, significantly reversed the protective effects of SFEE against H2O2-induced growth inhibition and ROS generation in C2C12 cells. Additional experiments revealed that the potential of the SFEE to induce HO-1 expression and protect against H2O2-mediated cellular damage was abrogated by transient transfection with Nrf2-specific small interfering RNA, suggesting that the SFEE protected C2C12 cells against oxidative stress-induced injury through the Nrf2/HO-1 pathway. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/25493944/Nrf2_mediated_HO_1_induction_contributes_to_antioxidant_capacity_of_a_Schisandrae_Fructus_ethanol_extract_in_C2C12_myoblasts_ L2 - https://www.mdpi.com/resolver?pii=nu6125667 DB - PRIME DP - Unbound Medicine ER -