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Jasmonic acid-isoleucine formation in grapevine (Vitis vinifera L.) by two enzymes with distinct transcription profiles.
J Integr Plant Biol. 2015 Jul; 57(7):618-27.JI

Abstract

The plant hormone jasmonic acid (JA) is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucial step in the JA signaling pathway since only JA-Ile is recognized by the jasmonate receptor. The conjugation reaction is catalyzed by JA-amido synthetases, belonging to the family of Gretchen Hagen3 (GH3) proteins. Here, in vitro studies of two grapevine (Vitis vinifera L. cv Shiraz) GH3 enzymes, VvGH3-7 and VvGH3-9, demonstrated JA-conjugating activities with an overlapping range of amino acid substrates, including isoleucine. Expression studies of the corresponding genes in grape berries combined with JA and JA-Ile measurements suggested a primary role for JA signaling in fruit set and cell division and did not support an involvement of JA in the ripening process. In response to methyl JA (MeJA) treatment, and in wounded and unwounded (distal) leaves, VvGH3-9 transcripts accumulated, indicating a participation in the JA response. In contrast, VvGH3-7 was unresponsive to MeJA and local wounding, demonstrating a differential transcriptional regulation of VvGH3-7 and VvGH3-9. The transient induction of VvGH3-7 in unwounded, distal leaves was suggestive of the involvement of an unknown mobile wound signal.

Authors+Show Affiliations

CSIRO Agriculture Flagship, Glen Osmond, South Australia, 5064, Australia.CSIRO Agriculture Flagship, Glen Osmond, South Australia, 5064, Australia.CSIRO Agriculture Flagship, Glen Osmond, South Australia, 5064, Australia.CSIRO Agriculture Flagship, Glen Osmond, South Australia, 5064, Australia.CSIRO Agriculture Flagship, Glen Osmond, South Australia, 5064, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25494944

Citation

Böttcher, Christine, et al. "Jasmonic Acid-isoleucine Formation in Grapevine (Vitis Vinifera L.) By Two Enzymes With Distinct Transcription Profiles." Journal of Integrative Plant Biology, vol. 57, no. 7, 2015, pp. 618-27.
Böttcher C, Burbidge CA, di Rienzo V, et al. Jasmonic acid-isoleucine formation in grapevine (Vitis vinifera L.) by two enzymes with distinct transcription profiles. J Integr Plant Biol. 2015;57(7):618-27.
Böttcher, C., Burbidge, C. A., di Rienzo, V., Boss, P. K., & Davies, C. (2015). Jasmonic acid-isoleucine formation in grapevine (Vitis vinifera L.) by two enzymes with distinct transcription profiles. Journal of Integrative Plant Biology, 57(7), 618-27. https://doi.org/10.1111/jipb.12321
Böttcher C, et al. Jasmonic Acid-isoleucine Formation in Grapevine (Vitis Vinifera L.) By Two Enzymes With Distinct Transcription Profiles. J Integr Plant Biol. 2015;57(7):618-27. PubMed PMID: 25494944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Jasmonic acid-isoleucine formation in grapevine (Vitis vinifera L.) by two enzymes with distinct transcription profiles. AU - Böttcher,Christine, AU - Burbidge,Crista A, AU - di Rienzo,Valentina, AU - Boss,Paul K, AU - Davies,Christopher, Y1 - 2015/02/12/ PY - 2014/10/06/received PY - 2014/12/08/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2016/3/29/medline KW - Berry development KW - GH3 KW - grapevine KW - jasmonic acid KW - wound response SP - 618 EP - 27 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 57 IS - 7 N2 - The plant hormone jasmonic acid (JA) is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucial step in the JA signaling pathway since only JA-Ile is recognized by the jasmonate receptor. The conjugation reaction is catalyzed by JA-amido synthetases, belonging to the family of Gretchen Hagen3 (GH3) proteins. Here, in vitro studies of two grapevine (Vitis vinifera L. cv Shiraz) GH3 enzymes, VvGH3-7 and VvGH3-9, demonstrated JA-conjugating activities with an overlapping range of amino acid substrates, including isoleucine. Expression studies of the corresponding genes in grape berries combined with JA and JA-Ile measurements suggested a primary role for JA signaling in fruit set and cell division and did not support an involvement of JA in the ripening process. In response to methyl JA (MeJA) treatment, and in wounded and unwounded (distal) leaves, VvGH3-9 transcripts accumulated, indicating a participation in the JA response. In contrast, VvGH3-7 was unresponsive to MeJA and local wounding, demonstrating a differential transcriptional regulation of VvGH3-7 and VvGH3-9. The transient induction of VvGH3-7 in unwounded, distal leaves was suggestive of the involvement of an unknown mobile wound signal. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/25494944/Jasmonic_acid_isoleucine_formation_in_grapevine__Vitis_vinifera_L___by_two_enzymes_with_distinct_transcription_profiles_ L2 - https://doi.org/10.1111/jipb.12321 DB - PRIME DP - Unbound Medicine ER -