Tags

Type your tag names separated by a space and hit enter

Target-triggering multiple-cycle amplification strategy for ultrasensitive detection of adenosine based on surface plasma resonance techniques.
Anal Chem. 2015 Jan 20; 87(2):929-36.AC

Abstract

An ultrasensitive protocol for surface plasma resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs-SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the "DNA trigger" of the downstream cycle (NESA and HCR) to generate further signal amplification, resulting in the immobilization of abundant Au NPs-SA on the gold substrate, and thus significant SPR enhancement is achieved due to the electronic coupling interaction between the localized surface plasma of Au NPs and the surface plasma wave. This detection method exhibits excellent specificity and sensitivity toward adenosine with a detection limit of 4 fM. The high sensitivity and specificity make this method a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yao GH
Department of Chemistry and ‡School of Life Sciences, Nanchang University , Nanchang 330031, People's Republic of China.
Liang RP
No affiliation info available
Yu XD
No affiliation info available
Huang CF
No affiliation info available
Zhang L
No affiliation info available
Qiu JD
No affiliation info available

MeSH

AdenosineAptamers, NucleotideBiosensing TechniquesDNAGoldHumansLimit of DetectionMetal NanoparticlesNucleic Acid HybridizationSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25494977

Citation

Yao, Gui-Hong, et al. "Target-triggering Multiple-cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based On Surface Plasma Resonance Techniques." Analytical Chemistry, vol. 87, no. 2, 2015, pp. 929-36.
Yao GH, Liang RP, Yu XD, et al. Target-triggering multiple-cycle amplification strategy for ultrasensitive detection of adenosine based on surface plasma resonance techniques. Anal Chem. 2015;87(2):929-36.
Yao, G. H., Liang, R. P., Yu, X. D., Huang, C. F., Zhang, L., & Qiu, J. D. (2015). Target-triggering multiple-cycle amplification strategy for ultrasensitive detection of adenosine based on surface plasma resonance techniques. Analytical Chemistry, 87(2), 929-36. https://doi.org/10.1021/ac503016f
Yao GH, et al. Target-triggering Multiple-cycle Amplification Strategy for Ultrasensitive Detection of Adenosine Based On Surface Plasma Resonance Techniques. Anal Chem. 2015 Jan 20;87(2):929-36. PubMed PMID: 25494977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Target-triggering multiple-cycle amplification strategy for ultrasensitive detection of adenosine based on surface plasma resonance techniques. AU - Yao,Gui-Hong, AU - Liang,Ru-Ping, AU - Yu,Xiang-Dan, AU - Huang,Chun-Fang, AU - Zhang,Li, AU - Qiu,Jian-Ding, Y1 - 2014/12/24/ PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2015/9/15/medline SP - 929 EP - 36 JF - Analytical chemistry JO - Anal Chem VL - 87 IS - 2 N2 - An ultrasensitive protocol for surface plasma resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs-SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the entire circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the "DNA trigger" of the downstream cycle (NESA and HCR) to generate further signal amplification, resulting in the immobilization of abundant Au NPs-SA on the gold substrate, and thus significant SPR enhancement is achieved due to the electronic coupling interaction between the localized surface plasma of Au NPs and the surface plasma wave. This detection method exhibits excellent specificity and sensitivity toward adenosine with a detection limit of 4 fM. The high sensitivity and specificity make this method a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/25494977/Target_triggering_multiple_cycle_amplification_strategy_for_ultrasensitive_detection_of_adenosine_based_on_surface_plasma_resonance_techniques_ DB - PRIME DP - Unbound Medicine ER -