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Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization.
Aquat Toxicol. 2015 Feb; 159:36-43.AT

Abstract

Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto Crassostrea gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment.

Authors+Show Affiliations

Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France; Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France. Electronic address: audrey.barranger@ifremer.fr.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France. Electronic address: abdellah.benabdelmouna@ifremer.fr.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, 17390 La Tremblade, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25498420

Citation

Barranger, Audrey, et al. "Parental Exposure to Environmental Concentrations of Diuron Leads to Aneuploidy in Embryos of the Pacific Oyster, as Evidenced By Fluorescent in Situ Hybridization." Aquatic Toxicology (Amsterdam, Netherlands), vol. 159, 2015, pp. 36-43.
Barranger A, Benabdelmouna A, Dégremont L, et al. Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization. Aquat Toxicol. 2015;159:36-43.
Barranger, A., Benabdelmouna, A., Dégremont, L., Burgeot, T., & Akcha, F. (2015). Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization. Aquatic Toxicology (Amsterdam, Netherlands), 159, 36-43. https://doi.org/10.1016/j.aquatox.2014.11.011
Barranger A, et al. Parental Exposure to Environmental Concentrations of Diuron Leads to Aneuploidy in Embryos of the Pacific Oyster, as Evidenced By Fluorescent in Situ Hybridization. Aquat Toxicol. 2015;159:36-43. PubMed PMID: 25498420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization. AU - Barranger,Audrey, AU - Benabdelmouna,Abdellah, AU - Dégremont,Lionel, AU - Burgeot,Thierry, AU - Akcha,Farida, Y1 - 2014/11/24/ PY - 2014/07/25/received PY - 2014/10/29/revised PY - 2014/11/13/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2015/4/2/medline KW - Aneuploidy KW - Crassostrea gigas. KW - Diuron KW - FISH SP - 36 EP - 43 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 159 N2 - Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto Crassostrea gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/25498420/Parental_exposure_to_environmental_concentrations_of_diuron_leads_to_aneuploidy_in_embryos_of_the_Pacific_oyster_as_evidenced_by_fluorescent_in_situ_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(14)00347-6 DB - PRIME DP - Unbound Medicine ER -