Tags

Type your tag names separated by a space and hit enter

Au nanoparticle/graphene nanocomposite as a platform for the sensitive detection of NADH in human urine.
Biosens Bioelectron. 2015 Apr 15; 66:474-80.BB

Abstract

Here we report on a facile, rapid, sensitive, selective and highly stable electrochemical sensing platform for β-nicotinamide adenine dinucleotide (NADH) based on uncapped Au nanoparticle/reduced graphene oxide (rGO) nanocomposites without the aid of any redox mediators and enzymes. The Au nanoparticle/rGO composite sensing platform was directly formed on a glassy carbon electrode through an in situ electrochemical reduction of GO and Au(3+) with a 100% usage of the precursors. The as-prepared Au nanoparticle/rGO composites demonstrated excellent direct electrocatalytic oxidation toward NADH, providing a large electrochemical active surface area as well as a favorable environment for electron transfer from NADH to the electrode via the enhanced mobility of charge carriers. The Au nanoparticle/rGO composites offered a ~2.3 times higher electrocatalytic current density with a negative shift of 112mV, in comparison to Au nanoparticles. The sensor developed in this study displayed a high sensitivity of 0.916µA/µMcm(2) and a wide linear range of from 50nM to 500µM with a limit of detection of 1.13nM (S/N=3). The interferences from the common interferents such as glutathione, glucose, ascorbic acid and quanine were negligible. The prepared sensor was further tested for the determination of NADH in human urine samples, showing the Au nanoparticle/rGO nanocomposites simultaneously formed by one-step electrochemical reduction have promising biomedical applications.

Authors+Show Affiliations

Department of Chemistry, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada P7B 5E1.Department of Chemistry, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada P7B 5E1.Department of Chemistry, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada P7B 5E1. Electronic address: aicheng.chen@lakeheadu.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25499660

Citation

Govindhan, Maduraiveeran, et al. "Au Nanoparticle/graphene Nanocomposite as a Platform for the Sensitive Detection of NADH in Human Urine." Biosensors & Bioelectronics, vol. 66, 2015, pp. 474-80.
Govindhan M, Amiri M, Chen A. Au nanoparticle/graphene nanocomposite as a platform for the sensitive detection of NADH in human urine. Biosens Bioelectron. 2015;66:474-80.
Govindhan, M., Amiri, M., & Chen, A. (2015). Au nanoparticle/graphene nanocomposite as a platform for the sensitive detection of NADH in human urine. Biosensors & Bioelectronics, 66, 474-80. https://doi.org/10.1016/j.bios.2014.12.012
Govindhan M, Amiri M, Chen A. Au Nanoparticle/graphene Nanocomposite as a Platform for the Sensitive Detection of NADH in Human Urine. Biosens Bioelectron. 2015 Apr 15;66:474-80. PubMed PMID: 25499660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Au nanoparticle/graphene nanocomposite as a platform for the sensitive detection of NADH in human urine. AU - Govindhan,Maduraiveeran, AU - Amiri,Mona, AU - Chen,Aicheng, Y1 - 2014/12/03/ PY - 2014/09/30/received PY - 2014/12/01/revised PY - 2014/12/02/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2015/9/1/medline KW - Au nanoparticle KW - Biosensor KW - Electrocatalysis KW - Graphene KW - Modified electrode KW - NADH SP - 474 EP - 80 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 66 N2 - Here we report on a facile, rapid, sensitive, selective and highly stable electrochemical sensing platform for β-nicotinamide adenine dinucleotide (NADH) based on uncapped Au nanoparticle/reduced graphene oxide (rGO) nanocomposites without the aid of any redox mediators and enzymes. The Au nanoparticle/rGO composite sensing platform was directly formed on a glassy carbon electrode through an in situ electrochemical reduction of GO and Au(3+) with a 100% usage of the precursors. The as-prepared Au nanoparticle/rGO composites demonstrated excellent direct electrocatalytic oxidation toward NADH, providing a large electrochemical active surface area as well as a favorable environment for electron transfer from NADH to the electrode via the enhanced mobility of charge carriers. The Au nanoparticle/rGO composites offered a ~2.3 times higher electrocatalytic current density with a negative shift of 112mV, in comparison to Au nanoparticles. The sensor developed in this study displayed a high sensitivity of 0.916µA/µMcm(2) and a wide linear range of from 50nM to 500µM with a limit of detection of 1.13nM (S/N=3). The interferences from the common interferents such as glutathione, glucose, ascorbic acid and quanine were negligible. The prepared sensor was further tested for the determination of NADH in human urine samples, showing the Au nanoparticle/rGO nanocomposites simultaneously formed by one-step electrochemical reduction have promising biomedical applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25499660/Au_nanoparticle/graphene_nanocomposite_as_a_platform_for_the_sensitive_detection_of_NADH_in_human_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00954-3 DB - PRIME DP - Unbound Medicine ER -