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Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS.
Clin Biochem. 2015 Feb; 48(3):189-91.CB

Abstract

OBJECTIVES

A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes.

DESIGN AND METHODS

Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min.

RESULTS

The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%).

CONCLUSION

The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).

Authors+Show Affiliations

Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 6, 128 43 Prague 2, Czech Republic.Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 6, 128 43 Prague 2, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25500419

Citation

Hložek, Tomáš, et al. "Simultaneous and Cost-effective Determination of Ethylene Glycol and Glycolic Acid in Human Serum and Urine for Emergency Toxicology By GC-MS." Clinical Biochemistry, vol. 48, no. 3, 2015, pp. 189-91.
Hložek T, Bursová M, Čabala R. Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS. Clin Biochem. 2015;48(3):189-91.
Hložek, T., Bursová, M., & Čabala, R. (2015). Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS. Clinical Biochemistry, 48(3), 189-91. https://doi.org/10.1016/j.clinbiochem.2014.12.002
Hložek T, Bursová M, Čabala R. Simultaneous and Cost-effective Determination of Ethylene Glycol and Glycolic Acid in Human Serum and Urine for Emergency Toxicology By GC-MS. Clin Biochem. 2015;48(3):189-91. PubMed PMID: 25500419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS. AU - Hložek,Tomáš, AU - Bursová,Miroslava, AU - Čabala,Radomír, Y1 - 2014/12/10/ PY - 2014/10/14/received PY - 2014/11/28/revised PY - 2014/12/01/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2015/10/9/medline KW - Chloroformate KW - GC-MS KW - Glycolic acid KW - Glycols KW - Toxicology SP - 189 EP - 91 JF - Clinical biochemistry JO - Clin Biochem VL - 48 IS - 3 N2 - OBJECTIVES: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. DESIGN AND METHODS: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. RESULTS: The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%). CONCLUSION: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid). SN - 1873-2933 UR - https://www.unboundmedicine.com/medline/citation/25500419/Simultaneous_and_cost_effective_determination_of_ethylene_glycol_and_glycolic_acid_in_human_serum_and_urine_for_emergency_toxicology_by_GC_MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-9120(14)00785-1 DB - PRIME DP - Unbound Medicine ER -