Abstract
OBJECTIVES
A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes.
DESIGN AND METHODS
Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min.
RESULTS
The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%).
CONCLUSION
The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).
TY - JOUR
T1 - Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS.
AU - Hložek,Tomáš,
AU - Bursová,Miroslava,
AU - Čabala,Radomír,
Y1 - 2014/12/10/
PY - 2014/10/14/received
PY - 2014/11/28/revised
PY - 2014/12/01/accepted
PY - 2014/12/16/entrez
PY - 2014/12/17/pubmed
PY - 2015/10/9/medline
KW - Chloroformate
KW - GC-MS
KW - Glycolic acid
KW - Glycols
KW - Toxicology
SP - 189
EP - 91
JF - Clinical biochemistry
JO - Clin Biochem
VL - 48
IS - 3
N2 - OBJECTIVES: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. DESIGN AND METHODS: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. RESULTS: The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%). CONCLUSION: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).
SN - 1873-2933
UR - https://www.unboundmedicine.com/medline/citation/25500419/Simultaneous_and_cost_effective_determination_of_ethylene_glycol_and_glycolic_acid_in_human_serum_and_urine_for_emergency_toxicology_by_GC_MS_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-9120(14)00785-1
DB - PRIME
DP - Unbound Medicine
ER -
