Tags

Type your tag names separated by a space and hit enter

Effect of probiotic supplementation on bacterial translocation in common bile duct obstruction.
Pediatr Surg Int. 2015 Feb; 31(2):155-61.PS

Abstract

OBJECTIVE

To investigate the effects of probiotics on bacterial translocation in the obstructive common bile duct with comparison to an enteral product containing arginine and glutamine.

MATERIAL AND METHOD

In our study, 40 Sprague-Dawley rats each weighing 250-300 g were used. Animals in Group 1 (sham) were laparatomized and fed standard chow supplemented with physiologic saline at daily doses of 2 ml through orogastric tube for 7 days. Common bile ducts of the animals in the other groups were ligated with 3/0 silk sutures. Group 2 (control group) was fed standard chow supplemented with daily doses of 2 ml physiologic saline. Group 3 (probiotic group) was fed standard chow supplemented with a probiotic solution (Acidophilus plus) containing strains of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at a daily doses of 2 × 10(9) colony forming units (CFU). Group 4 (formula group) was fed only an enteral solution (Stresson Multi Fiber) containing glutamine, arginine and a medium-chain fatty acid at daily doses of 2 g/kg. At the end of the 7th day, all animals were relaparatomized, and to determine bacterial translocation, aerobic, and anaerobic cultures were obtained from the specimens of mesenteric lymph nodes, intestinal mucosa, and blood samples. Smear cultures prepared from caecum were examined to determine the number of CFU. Finally, for histological examination specimens were excised from terminal ileum, and oxidative damage was assessed in liver tissues. Afterwards all animals were killed.

RESULTS

Moderately lesser degrees of bacterial translocation, and mucosal damage were seen in Groups 3, and 4 relative to Group 2 (p < 0.05). In Group 4, any difference was not seen in the number of cecal bacteria relative to baseline values, while in Group 3, significant decrease in cecal colonization was seen. Among all groups, a significant difference between levels of malondialdehyde, and glutathione was not observed.

CONCLUSION

At the end of our study, we have concluded that both probiotics, and enteral diets which contain immunomodulators such as glutamine, and arginine alleviate bacterial translocation, and impairment of intestinal mucosa.

Authors+Show Affiliations

Paediatric Surgery, Haseki Resarch and Education Hospital, Istanbul, Turkey, fsarac75@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25503581

Citation

Sarac, Fatma, et al. "Effect of Probiotic Supplementation On Bacterial Translocation in Common Bile Duct Obstruction." Pediatric Surgery International, vol. 31, no. 2, 2015, pp. 155-61.
Sarac F, Salman T, Gun F, et al. Effect of probiotic supplementation on bacterial translocation in common bile duct obstruction. Pediatr Surg Int. 2015;31(2):155-61.
Sarac, F., Salman, T., Gun, F., Celik, A., Gurler, N., Dogru Abbasoglu, S., Olgac, V., & Saygili, A. (2015). Effect of probiotic supplementation on bacterial translocation in common bile duct obstruction. Pediatric Surgery International, 31(2), 155-61. https://doi.org/10.1007/s00383-014-3643-2
Sarac F, et al. Effect of Probiotic Supplementation On Bacterial Translocation in Common Bile Duct Obstruction. Pediatr Surg Int. 2015;31(2):155-61. PubMed PMID: 25503581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of probiotic supplementation on bacterial translocation in common bile duct obstruction. AU - Sarac,Fatma, AU - Salman,Tansu, AU - Gun,Feryal, AU - Celik,Alaaddin, AU - Gurler,Nezahat, AU - Dogru Abbasoglu,Semra, AU - Olgac,Vakur, AU - Saygili,Ayse, Y1 - 2014/12/13/ PY - 2014/11/18/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2015/10/3/medline SP - 155 EP - 61 JF - Pediatric surgery international JO - Pediatr Surg Int VL - 31 IS - 2 N2 - OBJECTIVE: To investigate the effects of probiotics on bacterial translocation in the obstructive common bile duct with comparison to an enteral product containing arginine and glutamine. MATERIAL AND METHOD: In our study, 40 Sprague-Dawley rats each weighing 250-300 g were used. Animals in Group 1 (sham) were laparatomized and fed standard chow supplemented with physiologic saline at daily doses of 2 ml through orogastric tube for 7 days. Common bile ducts of the animals in the other groups were ligated with 3/0 silk sutures. Group 2 (control group) was fed standard chow supplemented with daily doses of 2 ml physiologic saline. Group 3 (probiotic group) was fed standard chow supplemented with a probiotic solution (Acidophilus plus) containing strains of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at a daily doses of 2 × 10(9) colony forming units (CFU). Group 4 (formula group) was fed only an enteral solution (Stresson Multi Fiber) containing glutamine, arginine and a medium-chain fatty acid at daily doses of 2 g/kg. At the end of the 7th day, all animals were relaparatomized, and to determine bacterial translocation, aerobic, and anaerobic cultures were obtained from the specimens of mesenteric lymph nodes, intestinal mucosa, and blood samples. Smear cultures prepared from caecum were examined to determine the number of CFU. Finally, for histological examination specimens were excised from terminal ileum, and oxidative damage was assessed in liver tissues. Afterwards all animals were killed. RESULTS: Moderately lesser degrees of bacterial translocation, and mucosal damage were seen in Groups 3, and 4 relative to Group 2 (p < 0.05). In Group 4, any difference was not seen in the number of cecal bacteria relative to baseline values, while in Group 3, significant decrease in cecal colonization was seen. Among all groups, a significant difference between levels of malondialdehyde, and glutathione was not observed. CONCLUSION: At the end of our study, we have concluded that both probiotics, and enteral diets which contain immunomodulators such as glutamine, and arginine alleviate bacterial translocation, and impairment of intestinal mucosa. SN - 1437-9813 UR - https://www.unboundmedicine.com/medline/citation/25503581/Effect_of_probiotic_supplementation_on_bacterial_translocation_in_common_bile_duct_obstruction_ L2 - https://doi.org/10.1007/s00383-014-3643-2 DB - PRIME DP - Unbound Medicine ER -