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Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat.
Mol Cytogenet. 2014; 7(1):91.MC

Abstract

BACKGROUND

Species belonging to the genus Aegilops L. are an important source of genetic material for expanding genetic variability of wheat. Ae. triuncialis is an allotetraploid in this genus which was originated from hybridization of Ae. umbellulata and Ae. markgrafii (Greuter) Hammer. Although the Ae. triuncialis karyotype was thoroughly examined by conventional chromosome staining and Giemsa C-banding, it is still poorly characterized using FISH markers. The objective of this study was to test the fluorescence in situ hybridization (FISH) patterns of Ae. triuncialis (2n = 4x = 28, C(t)C(t)U(t)U(t)) chromosomes using different repetitive sequences and to compare the produced patterns to the chromosomes of its diploid ancestors, with the aim of establishing a generalized Ae. triuncialis idiogram and detection of Aegilops chromosomes in the background of wheat.

RESULTS

The probes pSc119.2-1, pTa535-1, pAs1-1, (CTT)10 and the 45S rDNA clone from wheat (pTa71) were hybridized to chromosomes of Ae. triuncialis and compared with its diploid progenitors (Ae. umbellulata Zhuk., 2n = 2x = 14, UU and Ae. markgrafii (Greuter) Hammer, 2n = 2x = 14, CC) and Ae. cylindrica Host. (2n = 4x = 28, D(c)D(c)C(c)C(c)), another tetraploid species containing the C-genome. Ae. cylindrica was further analyzed by genomic in situ hybridization (GISH) using C genome probe in order to identify any possible translocation.

CONCLUSIONS

In general, FISH patterns of the U(t)- and C(t)-genome chromosomes of Ae. triuncialis were similar to those of U- and C-genome chromosomes of the diploid progenitor species Ae. umbellulata and Ae. markgrafii respectively, although some differences were observed. Two major 45S rDNA loci were revealed in the short arm of chromosomes A and C, of the C(t) genome which correspond to homoeologous groups 1 and 5 respectively. Minor 45S rDNA loci were mapped on the short arm of chromosomes 1U(t) and 5U(t). GISH analysis revealed three different non-reciprocal homologous or heterologous translocations between C(c) and D(c) chromosomes in all studied accessions of Ae. cylindrica.

Authors+Show Affiliations

Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, P. O. Box 66177-15175, Sanandaj, Iran.Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Corrensstraβe 3, 06466 Stadt Seeland, Germany.N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25505933

Citation

Mirzaghaderi, Ghader, et al. "Molecular-cytogenetic Analysis of Aegilops Triuncialis and Identification of Its Chromosomes in the Background of Wheat." Molecular Cytogenetics, vol. 7, no. 1, 2014, p. 91.
Mirzaghaderi G, Houben A, Badaeva ED. Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat. Mol Cytogenet. 2014;7(1):91.
Mirzaghaderi, G., Houben, A., & Badaeva, E. D. (2014). Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat. Molecular Cytogenetics, 7(1), 91. https://doi.org/10.1186/s13039-014-0091-6
Mirzaghaderi G, Houben A, Badaeva ED. Molecular-cytogenetic Analysis of Aegilops Triuncialis and Identification of Its Chromosomes in the Background of Wheat. Mol Cytogenet. 2014;7(1):91. PubMed PMID: 25505933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat. AU - Mirzaghaderi,Ghader, AU - Houben,Andreas, AU - Badaeva,Ekaterina D, Y1 - 2014/12/02/ PY - 2014/10/12/received PY - 2014/11/20/accepted PY - 2014/12/16/entrez PY - 2014/12/17/pubmed PY - 2014/12/17/medline KW - Aegilops triuncialis KW - Allopolyploidization KW - Chromosome marker KW - Evolution KW - Wheat–Aegilops triuncialis hybrid SP - 91 EP - 91 JF - Molecular cytogenetics JO - Mol Cytogenet VL - 7 IS - 1 N2 - BACKGROUND: Species belonging to the genus Aegilops L. are an important source of genetic material for expanding genetic variability of wheat. Ae. triuncialis is an allotetraploid in this genus which was originated from hybridization of Ae. umbellulata and Ae. markgrafii (Greuter) Hammer. Although the Ae. triuncialis karyotype was thoroughly examined by conventional chromosome staining and Giemsa C-banding, it is still poorly characterized using FISH markers. The objective of this study was to test the fluorescence in situ hybridization (FISH) patterns of Ae. triuncialis (2n = 4x = 28, C(t)C(t)U(t)U(t)) chromosomes using different repetitive sequences and to compare the produced patterns to the chromosomes of its diploid ancestors, with the aim of establishing a generalized Ae. triuncialis idiogram and detection of Aegilops chromosomes in the background of wheat. RESULTS: The probes pSc119.2-1, pTa535-1, pAs1-1, (CTT)10 and the 45S rDNA clone from wheat (pTa71) were hybridized to chromosomes of Ae. triuncialis and compared with its diploid progenitors (Ae. umbellulata Zhuk., 2n = 2x = 14, UU and Ae. markgrafii (Greuter) Hammer, 2n = 2x = 14, CC) and Ae. cylindrica Host. (2n = 4x = 28, D(c)D(c)C(c)C(c)), another tetraploid species containing the C-genome. Ae. cylindrica was further analyzed by genomic in situ hybridization (GISH) using C genome probe in order to identify any possible translocation. CONCLUSIONS: In general, FISH patterns of the U(t)- and C(t)-genome chromosomes of Ae. triuncialis were similar to those of U- and C-genome chromosomes of the diploid progenitor species Ae. umbellulata and Ae. markgrafii respectively, although some differences were observed. Two major 45S rDNA loci were revealed in the short arm of chromosomes A and C, of the C(t) genome which correspond to homoeologous groups 1 and 5 respectively. Minor 45S rDNA loci were mapped on the short arm of chromosomes 1U(t) and 5U(t). GISH analysis revealed three different non-reciprocal homologous or heterologous translocations between C(c) and D(c) chromosomes in all studied accessions of Ae. cylindrica. SN - 1755-8166 UR - https://www.unboundmedicine.com/medline/citation/25505933/Molecular_cytogenetic_analysis_of_Aegilops_triuncialis_and_identification_of_its_chromosomes_in_the_background_of_wheat_ L2 - https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-014-0091-6 DB - PRIME DP - Unbound Medicine ER -
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