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Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids.
Mol Cell Biol. 1989 Sep; 9(9):3999-4008.MC

Abstract

Transcription of the mouse mammary tumor virus DNA is known to be induced by several steroid hormones. Using chimeric MMTV plasmids containing mutations within the hormone regulatory element, we have previously studied the regions required for the glucocorticoid response in mouse fibroblasts. Here we report the characterization of elements essential for the stimulation by progestins and androgens as compared with glucocorticoids. The same set of mutant plasmids was transfected into the human mammary tumor cell line T47D, and the specific transcripts were analyzed by an S1 nuclease protection assay. Androgen-mediated stimulation, although weak, showed an extended sensitivity to mutations, with a slight preference for the proximal region. The results with progestin suggest that sequences within all the described sites protected by the receptor in vitro are required and that the promoter-proximal region (-128 to -78 from the RNA start site) is more important than the distal one (-190 to -160). Moreover, a binding site for nuclear factor I was not required for the progestin response, whereas it was required for glucocorticoids. Thus, the various steroid receptors play a role in the differential regulation of mouse mammary tumor virus transcription by recognizing distinct sequence differences in the hormone regulatory element and interacting with different factors bound to the promoter.

Authors+Show Affiliations

Swiss Institute for Experimental Cancer Research, Epalinges.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2550809

Citation

Gowland, P L., and E Buetti. "Mutations in the Hormone Regulatory Element of Mouse Mammary Tumor Virus Differentially Affect the Response to Progestins, Androgens, and Glucocorticoids." Molecular and Cellular Biology, vol. 9, no. 9, 1989, pp. 3999-4008.
Gowland PL, Buetti E. Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids. Mol Cell Biol. 1989;9(9):3999-4008.
Gowland, P. L., & Buetti, E. (1989). Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids. Molecular and Cellular Biology, 9(9), 3999-4008.
Gowland PL, Buetti E. Mutations in the Hormone Regulatory Element of Mouse Mammary Tumor Virus Differentially Affect the Response to Progestins, Androgens, and Glucocorticoids. Mol Cell Biol. 1989;9(9):3999-4008. PubMed PMID: 2550809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids. AU - Gowland,P L, AU - Buetti,E, PY - 1989/9/1/pubmed PY - 1989/9/1/medline PY - 1989/9/1/entrez SP - 3999 EP - 4008 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 9 IS - 9 N2 - Transcription of the mouse mammary tumor virus DNA is known to be induced by several steroid hormones. Using chimeric MMTV plasmids containing mutations within the hormone regulatory element, we have previously studied the regions required for the glucocorticoid response in mouse fibroblasts. Here we report the characterization of elements essential for the stimulation by progestins and androgens as compared with glucocorticoids. The same set of mutant plasmids was transfected into the human mammary tumor cell line T47D, and the specific transcripts were analyzed by an S1 nuclease protection assay. Androgen-mediated stimulation, although weak, showed an extended sensitivity to mutations, with a slight preference for the proximal region. The results with progestin suggest that sequences within all the described sites protected by the receptor in vitro are required and that the promoter-proximal region (-128 to -78 from the RNA start site) is more important than the distal one (-190 to -160). Moreover, a binding site for nuclear factor I was not required for the progestin response, whereas it was required for glucocorticoids. Thus, the various steroid receptors play a role in the differential regulation of mouse mammary tumor virus transcription by recognizing distinct sequence differences in the hormone regulatory element and interacting with different factors bound to the promoter. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/2550809/Mutations_in_the_hormone_regulatory_element_of_mouse_mammary_tumor_virus_differentially_affect_the_response_to_progestins_androgens_and_glucocorticoids_ L2 - https://journals.asm.org/doi/10.1128/mcb.9.9.3999-4008.1989?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -