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Iron, copper, and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress.
Free Radic Biol Med. 2015 Mar; 80:67-76.FR

Abstract

Due to their aerobic lifestyle, eukaryotic organisms have evolved different strategies to overcome oxidative stress. The recruitment of some specific metalloenzymes such as superoxide dismutases (SODs) and catalases (CATs) is of great importance for eliminating harmful reactive oxygen species (hydrogen peroxide and superoxide anion). Using the ligand HPClNOL {1-[bis(pyridin-2-ylmethyl)amino]-3-chloropropan-2-ol}, we have synthesized three coordination compounds containing iron(III), copper(II), and manganese(II) ions, which are also present in the active site of the above-noted metalloenzymes. These compounds were evaluated as SOD and CAT mimetics. The manganese and iron compounds showed both SOD and CAT activities, while copper showed only SOD activity. The copper and manganese in vitro SOD activities are very similar (IC50~0.4 μmol dm(-3)) and about 70-fold higher than those of iron. The manganese compound showed CAT activity higher than that of the iron species. Analyzing their capacity to protect Saccharomyces cerevisiae cells against oxidative stress (H2O2 and the O2(•-) radical), we observed that all compounds act as antioxidants, increasing the resistance of yeast cells mainly due to a reduction of lipid oxidation. Especially for the iron compound, the data indicate complete protection when wild-type cells were exposed to H2O2 or O2(•-) species. Interestingly, these compounds also compensate for both superoxide dismutase and catalase deficiencies; their antioxidant activity is metal ion dependent, in the order iron(III)>copper(II)>manganese(II). The protection mechanism employed by the complexes proved to be independent of the activation of transcription factors (such as Yap1, Hsf1, Msn2/Msn4) and protein synthesis. There is no direct relation between the in vitro and the in vivo antioxidant activities.

Authors+Show Affiliations

Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil.Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil.Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil.Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil.Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil.Laboratório de Ciência Físicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil.School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, 4072, Australia.Laboratório de Citotoxicidade e Genotoxicidade, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, RJ, Brazil. Electronic address: marcosdp@iq.ufrj.br.Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro - Campos dos Goytacazes, 28013-602, RJ, Brazil. Electronic address: adolfo@uenf.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25511255

Citation

Ribeiro, Thales P., et al. "Iron, Copper, and Manganese Complexes With in Vitro Superoxide Dismutase And/or Catalase Activities That Keep Saccharomyces Cerevisiae Cells Alive Under Severe Oxidative Stress." Free Radical Biology & Medicine, vol. 80, 2015, pp. 67-76.
Ribeiro TP, Fernandes C, Melo KV, et al. Iron, copper, and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress. Free Radic Biol Med. 2015;80:67-76.
Ribeiro, T. P., Fernandes, C., Melo, K. V., Ferreira, S. S., Lessa, J. A., Franco, R. W., Schenk, G., Pereira, M. D., & Horn, A. (2015). Iron, copper, and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress. Free Radical Biology & Medicine, 80, 67-76. https://doi.org/10.1016/j.freeradbiomed.2014.12.005
Ribeiro TP, et al. Iron, Copper, and Manganese Complexes With in Vitro Superoxide Dismutase And/or Catalase Activities That Keep Saccharomyces Cerevisiae Cells Alive Under Severe Oxidative Stress. Free Radic Biol Med. 2015;80:67-76. PubMed PMID: 25511255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron, copper, and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress. AU - Ribeiro,Thales P, AU - Fernandes,Christiane, AU - Melo,Karen V, AU - Ferreira,Sarah S, AU - Lessa,Josane A, AU - Franco,Roberto W A, AU - Schenk,Gerhard, AU - Pereira,Marcos D, AU - Horn,Adolfo,Jr Y1 - 2014/12/13/ PY - 2014/02/11/received PY - 2014/11/24/revised PY - 2014/12/01/accepted PY - 2014/12/17/entrez PY - 2014/12/17/pubmed PY - 2015/12/15/medline SP - 67 EP - 76 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 80 N2 - Due to their aerobic lifestyle, eukaryotic organisms have evolved different strategies to overcome oxidative stress. The recruitment of some specific metalloenzymes such as superoxide dismutases (SODs) and catalases (CATs) is of great importance for eliminating harmful reactive oxygen species (hydrogen peroxide and superoxide anion). Using the ligand HPClNOL {1-[bis(pyridin-2-ylmethyl)amino]-3-chloropropan-2-ol}, we have synthesized three coordination compounds containing iron(III), copper(II), and manganese(II) ions, which are also present in the active site of the above-noted metalloenzymes. These compounds were evaluated as SOD and CAT mimetics. The manganese and iron compounds showed both SOD and CAT activities, while copper showed only SOD activity. The copper and manganese in vitro SOD activities are very similar (IC50~0.4 μmol dm(-3)) and about 70-fold higher than those of iron. The manganese compound showed CAT activity higher than that of the iron species. Analyzing their capacity to protect Saccharomyces cerevisiae cells against oxidative stress (H2O2 and the O2(•-) radical), we observed that all compounds act as antioxidants, increasing the resistance of yeast cells mainly due to a reduction of lipid oxidation. Especially for the iron compound, the data indicate complete protection when wild-type cells were exposed to H2O2 or O2(•-) species. Interestingly, these compounds also compensate for both superoxide dismutase and catalase deficiencies; their antioxidant activity is metal ion dependent, in the order iron(III)>copper(II)>manganese(II). The protection mechanism employed by the complexes proved to be independent of the activation of transcription factors (such as Yap1, Hsf1, Msn2/Msn4) and protein synthesis. There is no direct relation between the in vitro and the in vivo antioxidant activities. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/25511255/Iron_copper_and_manganese_complexes_with_in_vitro_superoxide_dismutase_and/or_catalase_activities_that_keep_Saccharomyces_cerevisiae_cells_alive_under_severe_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(14)01401-4 DB - PRIME DP - Unbound Medicine ER -