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Molecular characterization of three Zika flaviviruses obtained from sylvatic mosquitoes in the Central African Republic.
Vector Borne Zoonotic Dis. 2014 Dec; 14(12):862-5.VB

Abstract

Zika virus (ZIKV) is an emerging pathogen belonging to the Spondweni serocomplex within the genus Flavivirus. It has been isolated from several mosquito species. Two lineages of ZIKV have been defined by polyprotein homology. Using high-throughput sequencing, we obtained and characterized three complete genomes of ZIKV isolated between 1976 and 1980 in the Central African Republic. The three viruses were isolated from two species of mosquito, Aedes africanus and Ae. opok. Two sequences from Ae. africanus had 99.9% nucleotide sequence identity and 100% amino acid identity, whereas the complete genome obtained from Ae. opok had 98.3% nucleotide identity and 99.4% amino acid identity with the other two genomes. Phylogenetic analysis based on the amino acid sequence of the polyprotein showed that the three ZIKV strains clustered together but diverged from all other ZIKV strains. Our molecular data suggest that a different subtype of West African ZIKV strains circulated in Aedes species in Central Africa.

Authors+Show Affiliations

1 Institut Pasteur de Bangui , Virology Department, Bangui, Central African Republic .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25514122

Citation

Berthet, Nicolas, et al. "Molecular Characterization of Three Zika Flaviviruses Obtained From Sylvatic Mosquitoes in the Central African Republic." Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), vol. 14, no. 12, 2014, pp. 862-5.
Berthet N, Nakouné E, Kamgang B, et al. Molecular characterization of three Zika flaviviruses obtained from sylvatic mosquitoes in the Central African Republic. Vector Borne Zoonotic Dis. 2014;14(12):862-5.
Berthet, N., Nakouné, E., Kamgang, B., Selekon, B., Descorps-Declère, S., Gessain, A., Manuguerra, J. C., & Kazanji, M. (2014). Molecular characterization of three Zika flaviviruses obtained from sylvatic mosquitoes in the Central African Republic. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 14(12), 862-5. https://doi.org/10.1089/vbz.2014.1607
Berthet N, et al. Molecular Characterization of Three Zika Flaviviruses Obtained From Sylvatic Mosquitoes in the Central African Republic. Vector Borne Zoonotic Dis. 2014;14(12):862-5. PubMed PMID: 25514122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of three Zika flaviviruses obtained from sylvatic mosquitoes in the Central African Republic. AU - Berthet,Nicolas, AU - Nakouné,Emmanuel, AU - Kamgang,Basile, AU - Selekon,Benjamin, AU - Descorps-Declère,Stéphane, AU - Gessain,Antoine, AU - Manuguerra,Jean-Claude, AU - Kazanji,Mirdad, PY - 2014/12/17/entrez PY - 2014/12/17/pubmed PY - 2015/8/27/medline KW - Aedes africanus KW - Aedes opok KW - Central Africa KW - Genomic characterization KW - Zika virus SP - 862 EP - 5 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis. VL - 14 IS - 12 N2 - Zika virus (ZIKV) is an emerging pathogen belonging to the Spondweni serocomplex within the genus Flavivirus. It has been isolated from several mosquito species. Two lineages of ZIKV have been defined by polyprotein homology. Using high-throughput sequencing, we obtained and characterized three complete genomes of ZIKV isolated between 1976 and 1980 in the Central African Republic. The three viruses were isolated from two species of mosquito, Aedes africanus and Ae. opok. Two sequences from Ae. africanus had 99.9% nucleotide sequence identity and 100% amino acid identity, whereas the complete genome obtained from Ae. opok had 98.3% nucleotide identity and 99.4% amino acid identity with the other two genomes. Phylogenetic analysis based on the amino acid sequence of the polyprotein showed that the three ZIKV strains clustered together but diverged from all other ZIKV strains. Our molecular data suggest that a different subtype of West African ZIKV strains circulated in Aedes species in Central Africa. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/25514122/Molecular_characterization_of_three_Zika_flaviviruses_obtained_from_sylvatic_mosquitoes_in_the_Central_African_Republic_ L2 - https://www.liebertpub.com/doi/full/10.1089/vbz.2014.1607?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -