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Molecular characterization and clonal relationships among Escherichia coli strains isolated from broiler chickens with colisepticemia.
Foodborne Pathog Dis. 2015 Jan; 12(1):74-83.FP

Abstract

This study characterized 52 Escherichia coli isolates from distinct diseased organs of 29 broiler chickens with clinical symptoms of colibacillosis in the Southern Brazilian state of Rio Grande do Sul. Thirty-eight isolates were highly virulent and 14 were virtually avirulent in 1-day-old chicks, yet all isolates harbored virulence factors characteristic of avian pathogenic E. coli (APEC), including those related to adhesion, iron acquisition, and serum resistance. E. coli reference collection phylogenetic typing showed that isolates belonged mostly to group D (39%), followed by group A (29%), group B1 (17%), and group B2 (15%). Phylogenetic analyses using the Amplified Ribosomal DNA Restriction Analysis and pulse-field gel electrophoresis methods were used to discriminate among isolates displaying the same serotype, revealing that five birds were infected with two distinct APEC strains. Among the 52 avian isolates, 2 were members of the pandemic E. coli O25:H4-B2-ST131 clone.

Authors+Show Affiliations

1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS, Brazil .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25514382

Citation

Barbieri, Nicolle Lima, et al. "Molecular Characterization and Clonal Relationships Among Escherichia Coli Strains Isolated From Broiler Chickens With Colisepticemia." Foodborne Pathogens and Disease, vol. 12, no. 1, 2015, pp. 74-83.
Barbieri NL, de Oliveira AL, Tejkowski TM, et al. Molecular characterization and clonal relationships among Escherichia coli strains isolated from broiler chickens with colisepticemia. Foodborne Pathog Dis. 2015;12(1):74-83.
Barbieri, N. L., de Oliveira, A. L., Tejkowski, T. M., Pavanelo, D. B., Matter, L. B., Pinheiro, S. R., Vaz, T. M., Nolan, L. K., Logue, C. M., de Brito, B. G., & Horn, F. (2015). Molecular characterization and clonal relationships among Escherichia coli strains isolated from broiler chickens with colisepticemia. Foodborne Pathogens and Disease, 12(1), 74-83. https://doi.org/10.1089/fpd.2014.1815
Barbieri NL, et al. Molecular Characterization and Clonal Relationships Among Escherichia Coli Strains Isolated From Broiler Chickens With Colisepticemia. Foodborne Pathog Dis. 2015;12(1):74-83. PubMed PMID: 25514382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and clonal relationships among Escherichia coli strains isolated from broiler chickens with colisepticemia. AU - Barbieri,Nicolle Lima, AU - de Oliveira,Aline Luísa, AU - Tejkowski,Thiago Moreira, AU - Pavanelo,Daniel Brisotto, AU - Matter,Letícia Beatriz, AU - Pinheiro,Sandra Regina Schincariol, AU - Vaz,Tânia Mara Ibelli, AU - Nolan,Lisa K, AU - Logue,Catherine M, AU - de Brito,Benito Guimarães, AU - Horn,Fabiana, Y1 - 2014/12/16/ PY - 2014/12/17/entrez PY - 2014/12/17/pubmed PY - 2015/9/12/medline SP - 74 EP - 83 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 12 IS - 1 N2 - This study characterized 52 Escherichia coli isolates from distinct diseased organs of 29 broiler chickens with clinical symptoms of colibacillosis in the Southern Brazilian state of Rio Grande do Sul. Thirty-eight isolates were highly virulent and 14 were virtually avirulent in 1-day-old chicks, yet all isolates harbored virulence factors characteristic of avian pathogenic E. coli (APEC), including those related to adhesion, iron acquisition, and serum resistance. E. coli reference collection phylogenetic typing showed that isolates belonged mostly to group D (39%), followed by group A (29%), group B1 (17%), and group B2 (15%). Phylogenetic analyses using the Amplified Ribosomal DNA Restriction Analysis and pulse-field gel electrophoresis methods were used to discriminate among isolates displaying the same serotype, revealing that five birds were infected with two distinct APEC strains. Among the 52 avian isolates, 2 were members of the pandemic E. coli O25:H4-B2-ST131 clone. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/25514382/Molecular_characterization_and_clonal_relationships_among_Escherichia_coli_strains_isolated_from_broiler_chickens_with_colisepticemia_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2014.1815?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -