TY - JOUR T1 - Signaling role of phospholipid hydroperoxide glutathione peroxidase (PHGPX) accompanying sensing of NaCl stress in etiolated sunflower seedling cotyledons. AU - Jain,Prachi, AU - Bhatla,Satish C, PY - 2014/12/18/entrez PY - 2014/12/18/pubmed PY - 2015/9/8/medline KW - CLSM, confocal laser scanning microscopy KW - GPX, glutathione peroxidase KW - LHPO, lipid hydroperoxide KW - PHGPX, phospholipid hydroperoxide glutathione peroxidase KW - POD,peroxidase KW - ROS, reactive oxygen species. KW - glutathione peroxidase KW - lipid hydroperoxides KW - phospholipid hydroperoxide glutathione peroxidase KW - salt stress KW - seedling cotyledons KW - sunflower SP - e977746 EP - e977746 JF - Plant signaling & behavior JO - Plant Signal Behav VL - 9 IS - 12 N2 - Sunflower seedlings subjected to 120 mM NaCl stress exhibit high total peroxidase activity, differential expression of its isoforms and accumulation of lipid hydroperoxides. This coincides with high specific activity of phospholipid hydroperoxide glutathione peroxidase (PHGPX) in the 10,000g supernatant from the homogenates of 2-6 d old seedling cotyledons. An upregulation of PHGPX activity by NaCl is evident from Western blot analysis. Confocal laser scanning microscopic (CLSM) analysis of sections of cotyledons incubated with anti-GPX4 (PHGPX) antibody highlights an enhanced cytosolic accumulation of PHGPX, particularly around the secretory canals. Present work, thus, highlights sensing of NaCl stress in sunflower seedlings in relation with lipid hydroperoxide accumulation and its scavenging through an upregulation of PHGPX activity in the cotyledons. SN - 1559-2324 UR - https://www.unboundmedicine.com/medline/citation/25517199/Signaling_role_of_phospholipid_hydroperoxide_glutathione_peroxidase__PHGPX__accompanying_sensing_of_NaCl_stress_in_etiolated_sunflower_seedling_cotyledons_ DB - PRIME DP - Unbound Medicine ER -