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Effect of hormones and development on the expression of the rat 1,25-dihydroxyvitamin D3 receptor gene. Comparison with calbindin gene expression.
J Biol Chem 1989; 264(29):17454-61JB

Abstract

We have used specific cDNAs to the rat vitamin D receptor (VDR) and to the mammalian vitamin D-dependent calcium-binding proteins (calbindin-D9k in intestine and calbindin-D28k in kidney) in order to obtain a better understanding of the regulation of the VDR gene and its relationship to calbindin gene expression. Hormonal regulation and development expression of the rat VDR gene were characterized by both Northern and slot blot analyses. Administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3; 25 ng/day for 7 days) to vitamin D-deficient rats resulted in an increase in calbindin mRNA in intestine and kidney but no change in VDR mRNA in these tissues. Vitamin D-deficient rats responded to dexamethasone treatment (100 micrograms/100 g of body weight/day for 4 days) with a 2.5-fold increase in intestinal VDR mRNA which was accompanied by a 4-fold decrease in intestinal calbindin-D9k mRNA. Developmental studies indicated a pronounced increase in renal VDR mRNA and calbindin-D28k mRNA between birth and 1 week of age. In the intestine, an induction of VDR and calbindin-D9k gene expression was observed at a later time, during the 3rd postnatal week (the period of increased duodenal active transport of calcium). Taken collectively, our data indicate that in the adult rat, target tissue response to hormone is not modified by a corresponding alteration in new receptor synthesis. However, developmental studies indicate that the induction of 1,25(OH)2D3 receptor mRNA is correlated with the induction of calbindin gene expression. Our results also demonstrate that glucocorticoid administration can result in an alteration in intestinal calbindin and VDR gene expression.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry-New Jersey Medical School, Newark 07103.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2551904

Citation

Huang, Y C., et al. "Effect of Hormones and Development On the Expression of the Rat 1,25-dihydroxyvitamin D3 Receptor Gene. Comparison With Calbindin Gene Expression." The Journal of Biological Chemistry, vol. 264, no. 29, 1989, pp. 17454-61.
Huang YC, Lee S, Stolz R, et al. Effect of hormones and development on the expression of the rat 1,25-dihydroxyvitamin D3 receptor gene. Comparison with calbindin gene expression. J Biol Chem. 1989;264(29):17454-61.
Huang, Y. C., Lee, S., Stolz, R., Gabrielides, C., Pansini-Porta, A., Bruns, M. E., ... Christakos, S. (1989). Effect of hormones and development on the expression of the rat 1,25-dihydroxyvitamin D3 receptor gene. Comparison with calbindin gene expression. The Journal of Biological Chemistry, 264(29), pp. 17454-61.
Huang YC, et al. Effect of Hormones and Development On the Expression of the Rat 1,25-dihydroxyvitamin D3 Receptor Gene. Comparison With Calbindin Gene Expression. J Biol Chem. 1989 Oct 15;264(29):17454-61. PubMed PMID: 2551904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of hormones and development on the expression of the rat 1,25-dihydroxyvitamin D3 receptor gene. Comparison with calbindin gene expression. AU - Huang,Y C, AU - Lee,S, AU - Stolz,R, AU - Gabrielides,C, AU - Pansini-Porta,A, AU - Bruns,M E, AU - Bruns,D E, AU - Miffin,T E, AU - Pike,J W, AU - Christakos,S, PY - 1989/10/15/pubmed PY - 1989/10/15/medline PY - 1989/10/15/entrez SP - 17454 EP - 61 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 264 IS - 29 N2 - We have used specific cDNAs to the rat vitamin D receptor (VDR) and to the mammalian vitamin D-dependent calcium-binding proteins (calbindin-D9k in intestine and calbindin-D28k in kidney) in order to obtain a better understanding of the regulation of the VDR gene and its relationship to calbindin gene expression. Hormonal regulation and development expression of the rat VDR gene were characterized by both Northern and slot blot analyses. Administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3; 25 ng/day for 7 days) to vitamin D-deficient rats resulted in an increase in calbindin mRNA in intestine and kidney but no change in VDR mRNA in these tissues. Vitamin D-deficient rats responded to dexamethasone treatment (100 micrograms/100 g of body weight/day for 4 days) with a 2.5-fold increase in intestinal VDR mRNA which was accompanied by a 4-fold decrease in intestinal calbindin-D9k mRNA. Developmental studies indicated a pronounced increase in renal VDR mRNA and calbindin-D28k mRNA between birth and 1 week of age. In the intestine, an induction of VDR and calbindin-D9k gene expression was observed at a later time, during the 3rd postnatal week (the period of increased duodenal active transport of calcium). Taken collectively, our data indicate that in the adult rat, target tissue response to hormone is not modified by a corresponding alteration in new receptor synthesis. However, developmental studies indicate that the induction of 1,25(OH)2D3 receptor mRNA is correlated with the induction of calbindin gene expression. Our results also demonstrate that glucocorticoid administration can result in an alteration in intestinal calbindin and VDR gene expression. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/2551904/Effect_of_hormones_and_development_on_the_expression_of_the_rat_125_dihydroxyvitamin_D3_receptor_gene__Comparison_with_calbindin_gene_expression_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=2551904 DB - PRIME DP - Unbound Medicine ER -