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Pomegranate ellagitannins inhibit α-glucosidase activity in vitro and reduce starch digestibility under simulated gastro-intestinal conditions.
Int J Food Sci Nutr. 2015 Feb; 66(1):85-92.IJ

Abstract

Pomegranate extract was tested for its ability to inhibit α-amylase and α-glucosidase activity. Pomegranate extract strongly inhibited rat intestinal α-glucosidase in vitro whereas it was a weak inhibitor of porcine α-amylase. The inhibitory activity was recovered in an ellagitannins-enriched fraction and punicalagin, punicalin, and ellagic acid were identified as α-glucosidase inhibitors (IC(50) of 140.2, 191.4, and 380.9 μmol/L, respectively). Kinetic analysis suggested that the pomegranate extract and ellagitannins inhibited α-glucosidase activity in a mixed mode. The inhibitory activity was demonstrated using an in vitro digestion system, mimicking the physiological gastro-intestinal condition, and potatoes as food rich in starch. Pre-incubation between ellagitannins and α-glucosidase increased the inhibitory activity, suggesting that they acted by binding to α-glucosidase. During digestion punicalin and punicalagin concentration decreased. Despite this loss, the pomegranate extract retained high inhibitory activity. This study suggests that pomegranate ellagitannins may inhibit α-glucosidase activity in vitro possibly affecting in vivo starch digestion.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bellesia A
Department of Life Sciences, University of Modena and Reggio Emilia , Reggio Emilia , Italy.
Verzelloni E
No affiliation info available
Tagliazucchi D
No affiliation info available

MeSH

AnimalsEnzyme InhibitorsGastrointestinal TractHydrolyzable TanninsLythraceaeModels, BiologicalPlant ExtractsPolyphenolsRatsStarchTandem Mass Spectrometryalpha-Glucosidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25519249

Citation

Bellesia, Andrea, et al. "Pomegranate Ellagitannins Inhibit Α-glucosidase Activity in Vitro and Reduce Starch Digestibility Under Simulated Gastro-intestinal Conditions." International Journal of Food Sciences and Nutrition, vol. 66, no. 1, 2015, pp. 85-92.
Bellesia A, Verzelloni E, Tagliazucchi D. Pomegranate ellagitannins inhibit α-glucosidase activity in vitro and reduce starch digestibility under simulated gastro-intestinal conditions. Int J Food Sci Nutr. 2015;66(1):85-92.
Bellesia, A., Verzelloni, E., & Tagliazucchi, D. (2015). Pomegranate ellagitannins inhibit α-glucosidase activity in vitro and reduce starch digestibility under simulated gastro-intestinal conditions. International Journal of Food Sciences and Nutrition, 66(1), 85-92. https://doi.org/10.3109/09637486.2014.953455
Bellesia A, Verzelloni E, Tagliazucchi D. Pomegranate Ellagitannins Inhibit Α-glucosidase Activity in Vitro and Reduce Starch Digestibility Under Simulated Gastro-intestinal Conditions. Int J Food Sci Nutr. 2015;66(1):85-92. PubMed PMID: 25519249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pomegranate ellagitannins inhibit α-glucosidase activity in vitro and reduce starch digestibility under simulated gastro-intestinal conditions. AU - Bellesia,Andrea, AU - Verzelloni,Elena, AU - Tagliazucchi,Davide, Y1 - 2014/12/18/ PY - 2014/12/19/entrez PY - 2014/12/19/pubmed PY - 2015/10/7/medline KW - Diabetes KW - ellagitannins KW - mass spectrometry KW - pomegranate KW - starch digestion SP - 85 EP - 92 JF - International journal of food sciences and nutrition JO - Int J Food Sci Nutr VL - 66 IS - 1 N2 - Pomegranate extract was tested for its ability to inhibit α-amylase and α-glucosidase activity. Pomegranate extract strongly inhibited rat intestinal α-glucosidase in vitro whereas it was a weak inhibitor of porcine α-amylase. The inhibitory activity was recovered in an ellagitannins-enriched fraction and punicalagin, punicalin, and ellagic acid were identified as α-glucosidase inhibitors (IC(50) of 140.2, 191.4, and 380.9 μmol/L, respectively). Kinetic analysis suggested that the pomegranate extract and ellagitannins inhibited α-glucosidase activity in a mixed mode. The inhibitory activity was demonstrated using an in vitro digestion system, mimicking the physiological gastro-intestinal condition, and potatoes as food rich in starch. Pre-incubation between ellagitannins and α-glucosidase increased the inhibitory activity, suggesting that they acted by binding to α-glucosidase. During digestion punicalin and punicalagin concentration decreased. Despite this loss, the pomegranate extract retained high inhibitory activity. This study suggests that pomegranate ellagitannins may inhibit α-glucosidase activity in vitro possibly affecting in vivo starch digestion. SN - 1465-3478 UR - https://www.unboundmedicine.com/medline/citation/25519249/Pomegranate_ellagitannins_inhibit_��_glucosidase_activity_in_vitro_and_reduce_starch_digestibility_under_simulated_gastro_intestinal_conditions_ DB - PRIME DP - Unbound Medicine ER -