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Roles of p53 and caspases in induction of apoptosis in MCF- 7 breast cancer cells treated with a methanolic extract of Nigella sativa seeds.
Asian Pac J Cancer Prev. 2014; 15(22):9655-60.AP

Abstract

BACKGROUND

Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells.

MATERIALS AND METHODS

Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose- and time-dependent study was performed. The IC50 was calculated using a Cell Titer Blue® viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR.

RESULTS

The IC50 of MCF-7 cells was 62.8 μL/mL. When MCF-7 cells were exposed to 50 μL/mL and 100 μL/mL NS for 24 h, 48 h and 72 h, microscopic examination (TUNEL assay) revealed a dose- and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time.

CONCLUSIONS

NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy.

Authors+Show Affiliations

Molecular Cancer Biology Research Lab, Dept. of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Saudi Arabia E-mail : alialshatwi@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25520084

Citation

Alhazmi, Mohammed I., et al. "Roles of P53 and Caspases in Induction of Apoptosis in MCF- 7 Breast Cancer Cells Treated With a Methanolic Extract of Nigella Sativa Seeds." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 15, no. 22, 2014, pp. 9655-60.
Alhazmi MI, Hasan TN, Shafi G, et al. Roles of p53 and caspases in induction of apoptosis in MCF- 7 breast cancer cells treated with a methanolic extract of Nigella sativa seeds. Asian Pac J Cancer Prev. 2014;15(22):9655-60.
Alhazmi, M. I., Hasan, T. N., Shafi, G., Al-Assaf, A. H., Alfawaz, M. A., & Alshatwi, A. A. (2014). Roles of p53 and caspases in induction of apoptosis in MCF- 7 breast cancer cells treated with a methanolic extract of Nigella sativa seeds. Asian Pacific Journal of Cancer Prevention : APJCP, 15(22), 9655-60.
Alhazmi MI, et al. Roles of P53 and Caspases in Induction of Apoptosis in MCF- 7 Breast Cancer Cells Treated With a Methanolic Extract of Nigella Sativa Seeds. Asian Pac J Cancer Prev. 2014;15(22):9655-60. PubMed PMID: 25520084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of p53 and caspases in induction of apoptosis in MCF- 7 breast cancer cells treated with a methanolic extract of Nigella sativa seeds. AU - Alhazmi,Mohammed I, AU - Hasan,Tarique N, AU - Shafi,Gowhar, AU - Al-Assaf,Abdullah H, AU - Alfawaz,Mohammed A, AU - Alshatwi,Ali A, PY - 2014/12/19/entrez PY - 2014/12/19/pubmed PY - 2015/8/25/medline SP - 9655 EP - 60 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 15 IS - 22 N2 - BACKGROUND: Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells. MATERIALS AND METHODS: Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose- and time-dependent study was performed. The IC50 was calculated using a Cell Titer Blue® viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR. RESULTS: The IC50 of MCF-7 cells was 62.8 μL/mL. When MCF-7 cells were exposed to 50 μL/mL and 100 μL/mL NS for 24 h, 48 h and 72 h, microscopic examination (TUNEL assay) revealed a dose- and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time. CONCLUSIONS: NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/25520084/Roles_of_p53_and_caspases_in_induction_of_apoptosis_in_MCF__7_breast_cancer_cells_treated_with_a_methanolic_extract_of_Nigella_sativa_seeds_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:25520084&key=2014.15.22.9655 DB - PRIME DP - Unbound Medicine ER -