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Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated with Breast Cancer.
Biomark Cancer. 2014; 6:37-47.BC

Abstract

Small noncoding RNAs circulating in the blood may serve as signaling molecules because of their ability to carry out a variety of cellular functions. We have previously described tRNA- and YRNA-derived small RNAs circulating as components of larger complexes in the blood of humans and mice; the characteristics of these small RNAs imply specific processing, secretion, and physiological regulation. In this study, we have asked if changes in the serum abundance of these tRNA and YRNA fragments are associated with a diagnosis of cancer. We used deep sequencing and informatics analysis to catalog small RNAs in the sera of breast cancer cases and normal controls. 5' tRNA halves and YRNA fragments are abundant in both groups, but we found that a breast cancer diagnosis is associated with changes in levels of specific subtypes. This prompted us to look at existing sequence datasets of serum small RNAs from 42 breast cancer cases, taken at the time of diagnosis. We find significant changes in the levels of specific 5' tRNA halves and YRNA fragments associated with clinicopathologic characteristics of the cancer. Although these findings do not establish causality, they suggest that circulating 5' tRNA halves and YRNA fragments with known cellular functions may participate in breast cancer syndromes and have potential as circulating biomarkers. Larger studies with multiple types of cancer are needed to adequately evaluate their potential use for the development of noninvasive cancer screening.

Authors+Show Affiliations

Department of Biochemistry, University of California at Riverside, Riverside, CA, USA. ; Center for Genetics, Children's Hospital Oakland Research Institute, Oakland, CA, USA.Department of Biochemistry, University of California at Riverside, Riverside, CA, USA.Department of Medical Education, California Northstate University, Elk Grove, CA, USA.Center for Genetics, Children's Hospital Oakland Research Institute, Oakland, CA, USA.Center for Genetics, Children's Hospital Oakland Research Institute, Oakland, CA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25520563

Citation

Dhahbi, Joseph M., et al. "Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated With Breast Cancer." Biomarkers in Cancer, vol. 6, 2014, pp. 37-47.
Dhahbi JM, Spindler SR, Atamna H, et al. Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated with Breast Cancer. Biomark Cancer. 2014;6:37-47.
Dhahbi, J. M., Spindler, S. R., Atamna, H., Boffelli, D., & Martin, D. I. (2014). Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated with Breast Cancer. Biomarkers in Cancer, 6, 37-47. https://doi.org/10.4137/BIC.S20764
Dhahbi JM, et al. Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated With Breast Cancer. Biomark Cancer. 2014;6:37-47. PubMed PMID: 25520563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deep Sequencing of Serum Small RNAs Identifies Patterns of 5' tRNA Half and YRNA Fragment Expression Associated with Breast Cancer. AU - Dhahbi,Joseph M, AU - Spindler,Stephen R, AU - Atamna,Hani, AU - Boffelli,Dario, AU - Martin,David Ik, Y1 - 2014/12/08/ PY - 2014/10/02/received PY - 2014/11/14/revised PY - 2014/11/17/accepted PY - 2014/12/19/entrez PY - 2014/12/19/pubmed PY - 2014/12/19/medline KW - Y RNA KW - YRNA fragments KW - breast cancer KW - circulating small RNAs KW - serum KW - tRNA derivatives KW - tRNA halves SP - 37 EP - 47 JF - Biomarkers in cancer JO - Biomark Cancer VL - 6 N2 - Small noncoding RNAs circulating in the blood may serve as signaling molecules because of their ability to carry out a variety of cellular functions. We have previously described tRNA- and YRNA-derived small RNAs circulating as components of larger complexes in the blood of humans and mice; the characteristics of these small RNAs imply specific processing, secretion, and physiological regulation. In this study, we have asked if changes in the serum abundance of these tRNA and YRNA fragments are associated with a diagnosis of cancer. We used deep sequencing and informatics analysis to catalog small RNAs in the sera of breast cancer cases and normal controls. 5' tRNA halves and YRNA fragments are abundant in both groups, but we found that a breast cancer diagnosis is associated with changes in levels of specific subtypes. This prompted us to look at existing sequence datasets of serum small RNAs from 42 breast cancer cases, taken at the time of diagnosis. We find significant changes in the levels of specific 5' tRNA halves and YRNA fragments associated with clinicopathologic characteristics of the cancer. Although these findings do not establish causality, they suggest that circulating 5' tRNA halves and YRNA fragments with known cellular functions may participate in breast cancer syndromes and have potential as circulating biomarkers. Larger studies with multiple types of cancer are needed to adequately evaluate their potential use for the development of noninvasive cancer screening. SN - 1179-299X UR - https://www.unboundmedicine.com/medline/citation/25520563/Deep_Sequencing_of_Serum_Small_RNAs_Identifies_Patterns_of_5'_tRNA_Half_and_YRNA_Fragment_Expression_Associated_with_Breast_Cancer_ L2 - http://journals.sagepub.com/doi/full/10.4137/BIC.S20764?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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