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Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice.
J Ethnopharmacol 2015; 161:214-23JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Fresh Portulaca oleracea L. (family: Portulacaceae; POL) has been used as a folk medicine for the treatment of diabetes mellitus for a long time. More bioactive components with higher activity could be retained in fresh medicinal herbs compared to the dried ones. The present study was conducted to compare different antidiabetic activity between fresh and dried POL, including hypoglycemic and antioxidant activities both in vivo and in vitro. Furthermore, in order to explore which components were responsible for the antidiabetic activity, the difference on chemical components between fresh and dried POL was analyzed and compared.

MATERIALS AND METHODS

Insulin-resistant HepG2 cells induced by insulin were used to evaluate the promoting effect of the fresh and dried POL on glucose utilization in vitro. Streptozotocin (STZ)-induced C57BL/6J diabetic mice were used to compare the differences on hypoglycemic and antioxidant activities of fresh and dried POL, including the fasting blood glucose, glucose tolerance, serum insulin level, malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in vivo. UPLC/Q-TOF-MS method was performed to analyze the difference of antidiabetic components between fresh and dried POL.

RESULTS

Compared with the dried POL extract, the fresh POL extract significantly increased the consumption of extracellular glucose in insulin-resistant HepG2 cells (P<0.05). In STZ-induced C57BL/6J diabetic mice, both fresh and dried extracts decreased markedly the fasting blood glucose (FBG) levels, and improved significantly oral glucose tolerance test (OGTT), as well as enhanced significantly insulin secretion and antioxidative activities (P<0.05; P<0.01). Furthermore, the fresh extract showed stronger antidiabetic activity (P<0.05). The UPLC/Q-TOF-MS analysis results also revealed that the relative contents of polyphenols and alkaloids in the fresh herbs were more abundant than those in the dried POL.

CONCLUSION

Our results indicated that both fresh and dried POL possessed antidiabetic activities, besides stronger activity was observed in the fresh herb. These findings provided evidence for the application and development of fresh POL in the treatment of diabetes mellitus.

Authors+Show Affiliations

Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu, China.Department of Intervention, Cancer Hospital of Jiangsu Province, Nanjing 210009, Jiangsu, China.Department of Intervention, Cancer Hospital of Jiangsu Province, Nanjing 210009, Jiangsu, China.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China. Electronic address: wenmoxiushi@163.com.Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, Jiangsu, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; College of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu, China. Electronic address: xiaobinjia_nj@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25523372

Citation

Gu, Jun-Fei, et al. "Comparison On Hypoglycemic and Antioxidant Activities of the Fresh and Dried Portulaca Oleracea L. in Insulin-resistant HepG2 Cells and Streptozotocin-induced C57BL/6J Diabetic Mice." Journal of Ethnopharmacology, vol. 161, 2015, pp. 214-23.
Gu JF, Zheng ZY, Yuan JR, et al. Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice. J Ethnopharmacol. 2015;161:214-23.
Gu, J. F., Zheng, Z. Y., Yuan, J. R., Zhao, B. J., Wang, C. F., Zhang, L., ... Jia, X. B. (2015). Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice. Journal of Ethnopharmacology, 161, pp. 214-23. doi:10.1016/j.jep.2014.12.002.
Gu JF, et al. Comparison On Hypoglycemic and Antioxidant Activities of the Fresh and Dried Portulaca Oleracea L. in Insulin-resistant HepG2 Cells and Streptozotocin-induced C57BL/6J Diabetic Mice. J Ethnopharmacol. 2015 Feb 23;161:214-23. PubMed PMID: 25523372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice. AU - Gu,Jun-Fei, AU - Zheng,Zhi-Yin, AU - Yuan,Jia-Rui, AU - Zhao,Bing-Jie, AU - Wang,Chun-Fei, AU - Zhang,Li, AU - Xu,Qing-Yu, AU - Yin,Guo-Wen, AU - Feng,Liang, AU - Jia,Xiao-Bin, Y1 - 2014/12/16/ PY - 2014/03/12/received PY - 2014/11/16/revised PY - 2014/12/01/accepted PY - 2014/12/20/entrez PY - 2014/12/20/pubmed PY - 2015/10/7/medline KW - Antioxidant KW - Components difference KW - Diabetes mellitus KW - Fresh and dried herbs KW - Hypoglycemic KW - Portulaca oleracea L SP - 214 EP - 23 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 161 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Fresh Portulaca oleracea L. (family: Portulacaceae; POL) has been used as a folk medicine for the treatment of diabetes mellitus for a long time. More bioactive components with higher activity could be retained in fresh medicinal herbs compared to the dried ones. The present study was conducted to compare different antidiabetic activity between fresh and dried POL, including hypoglycemic and antioxidant activities both in vivo and in vitro. Furthermore, in order to explore which components were responsible for the antidiabetic activity, the difference on chemical components between fresh and dried POL was analyzed and compared. MATERIALS AND METHODS: Insulin-resistant HepG2 cells induced by insulin were used to evaluate the promoting effect of the fresh and dried POL on glucose utilization in vitro. Streptozotocin (STZ)-induced C57BL/6J diabetic mice were used to compare the differences on hypoglycemic and antioxidant activities of fresh and dried POL, including the fasting blood glucose, glucose tolerance, serum insulin level, malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in vivo. UPLC/Q-TOF-MS method was performed to analyze the difference of antidiabetic components between fresh and dried POL. RESULTS: Compared with the dried POL extract, the fresh POL extract significantly increased the consumption of extracellular glucose in insulin-resistant HepG2 cells (P<0.05). In STZ-induced C57BL/6J diabetic mice, both fresh and dried extracts decreased markedly the fasting blood glucose (FBG) levels, and improved significantly oral glucose tolerance test (OGTT), as well as enhanced significantly insulin secretion and antioxidative activities (P<0.05; P<0.01). Furthermore, the fresh extract showed stronger antidiabetic activity (P<0.05). The UPLC/Q-TOF-MS analysis results also revealed that the relative contents of polyphenols and alkaloids in the fresh herbs were more abundant than those in the dried POL. CONCLUSION: Our results indicated that both fresh and dried POL possessed antidiabetic activities, besides stronger activity was observed in the fresh herb. These findings provided evidence for the application and development of fresh POL in the treatment of diabetes mellitus. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25523372/Comparison_on_hypoglycemic_and_antioxidant_activities_of_the_fresh_and_dried_Portulaca_oleracea_L__in_insulin_resistant_HepG2_cells_and_streptozotocin_induced_C57BL/6J_diabetic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(14)00867-8 DB - PRIME DP - Unbound Medicine ER -