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Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen.
Theor Appl Genet. 2015 Mar; 128(3):431-43.TA

Abstract

KEY MESSAGE

Mapping studies confirm that resistance to Ug99 race of stem rust pathogen in Aegilops tauschii accession Clae 25 is conditioned by Sr46 and markers linked to the gene were developed for marker-assisted selection. The race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal pathogen for wheat stem rust, is considered as a major threat to global wheat production. To address this threat, researchers across the world have been devoted to identifying TTKSK-resistant genes. Here, we report the identification and mapping of a stem rust resistance gene in Aegilops tauschii accession CIae 25 that confers resistance to TTKSK and the development of molecular markers for the gene. An F2 population of 710 plants from an Ae. tauschii cross CIae 25 × AL8/78 were first evaluated against race TPMKC. A set of 14 resistant and 116 susceptible F2:3 families from the F2 plants were then evaluated for their reactions to TTKSK. Based on the tests, 179 homozygous susceptible F2 plants were selected as the mapping population to identify the simple sequence repeat (SSR) and sequence tagged site (STS) markers linked to the gene by bulk segregant analysis. A dominant stem rust resistance gene was identified and mapped with 16 SSR and five new STS markers to the deletion bin 2DS5-0.47-1.00 of chromosome arm 2DS in which Sr46 was located. Molecular marker and stem rust tests on CIae 25 and two Ae. tauschii accessions carrying Sr46 confirmed that the gene in CIae 25 is Sr46. This study also demonstrated that Sr46 is temperature-sensitive being less effective at low temperatures. The marker validation indicated that two closely linked markers Xgwm210 and Xwmc111 can be used for marker-assisted selection of Sr46 in wheat breeding programs.

Authors+Show Affiliations

Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25523501

Citation

Yu, Guotai, et al. "Identification and Mapping of Sr46 From Aegilops Tauschii Accession CIae 25 Conferring Resistance to Race TTKSK (Ug99) of Wheat Stem Rust Pathogen." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 3, 2015, pp. 431-43.
Yu G, Zhang Q, Friesen TL, et al. Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen. Theor Appl Genet. 2015;128(3):431-43.
Yu, G., Zhang, Q., Friesen, T. L., Rouse, M. N., Jin, Y., Zhong, S., Rasmussen, J. B., Lagudah, E. S., & Xu, S. S. (2015). Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(3), 431-43. https://doi.org/10.1007/s00122-014-2442-4
Yu G, et al. Identification and Mapping of Sr46 From Aegilops Tauschii Accession CIae 25 Conferring Resistance to Race TTKSK (Ug99) of Wheat Stem Rust Pathogen. Theor Appl Genet. 2015;128(3):431-43. PubMed PMID: 25523501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen. AU - Yu,Guotai, AU - Zhang,Qijun, AU - Friesen,Timothy L, AU - Rouse,Matthew N, AU - Jin,Yue, AU - Zhong,Shaobin, AU - Rasmussen,Jack B, AU - Lagudah,Evans S, AU - Xu,Steven S, Y1 - 2014/12/19/ PY - 2014/06/04/received PY - 2014/12/06/accepted PY - 2014/12/20/entrez PY - 2014/12/20/pubmed PY - 2015/5/20/medline SP - 431 EP - 43 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 3 N2 - KEY MESSAGE: Mapping studies confirm that resistance to Ug99 race of stem rust pathogen in Aegilops tauschii accession Clae 25 is conditioned by Sr46 and markers linked to the gene were developed for marker-assisted selection. The race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal pathogen for wheat stem rust, is considered as a major threat to global wheat production. To address this threat, researchers across the world have been devoted to identifying TTKSK-resistant genes. Here, we report the identification and mapping of a stem rust resistance gene in Aegilops tauschii accession CIae 25 that confers resistance to TTKSK and the development of molecular markers for the gene. An F2 population of 710 plants from an Ae. tauschii cross CIae 25 × AL8/78 were first evaluated against race TPMKC. A set of 14 resistant and 116 susceptible F2:3 families from the F2 plants were then evaluated for their reactions to TTKSK. Based on the tests, 179 homozygous susceptible F2 plants were selected as the mapping population to identify the simple sequence repeat (SSR) and sequence tagged site (STS) markers linked to the gene by bulk segregant analysis. A dominant stem rust resistance gene was identified and mapped with 16 SSR and five new STS markers to the deletion bin 2DS5-0.47-1.00 of chromosome arm 2DS in which Sr46 was located. Molecular marker and stem rust tests on CIae 25 and two Ae. tauschii accessions carrying Sr46 confirmed that the gene in CIae 25 is Sr46. This study also demonstrated that Sr46 is temperature-sensitive being less effective at low temperatures. The marker validation indicated that two closely linked markers Xgwm210 and Xwmc111 can be used for marker-assisted selection of Sr46 in wheat breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/25523501/Identification_and_mapping_of_Sr46_from_Aegilops_tauschii_accession_CIae_25_conferring_resistance_to_race_TTKSK__Ug99__of_wheat_stem_rust_pathogen_ L2 - https://dx.doi.org/10.1007/s00122-014-2442-4 DB - PRIME DP - Unbound Medicine ER -