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Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats.
Neurosci Lett 2015; 587:51-6NL

Abstract

The underlying mechanism of isoflurane-induced cognitive dysfunction in older individuals is unknown. In this study, the effects of isoflurane exposure on the hippocampal blood-brain barrier (BBB) in aged rats were investigated because it was previously shown that BBB disruption involves in cognitive dysfunction. Twenty-month-old rats randomly received 1.5% isoflurane or vehicle gas as control. Hippocampal BBB ultrastructure was analyzed by transmission electron microscopy and expression of tight junction proteins was measured by western blot analysis. BBB permeability was detected with sodium fluorescein extravasation and further confirmed by immunoglobulin G immunohistochemistry. Spatial learning and memory were assessed by the Morris water maze test. Isoflurane anesthesia resulted in reversible time-dependent BBB ultrastructure morphological damage and significant decreases in expression of the tight junction proteins occludin, which contributed to sodium fluorescein and IgG leakage. Rats with isoflurane exposure also showed significant cognitive deficits in the Morris water maze test. This in vivo data indicate that occludin down-regulation may be one of the mediators of isoflurane-induced hippocampus BBB disruption, and may contribute to hippocampus-dependent cognitive impairment after isoflurane exposure in aged rats.

Authors+Show Affiliations

Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China.Department of Pathology, School of Basic Medical Sciences, Peking University, Beijing 100191, China. Electronic address: zhongyf@bjmu.edc.Neuroscience Research Institute, Department of Neurobiology, Peking University, Beijing 100191, China. Electronic address: dchui@bjmu.edu.Department of Anesthesiology, Peking University Third Hospital, Beijing 100191, China. Electronic address: puthmzk@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25524410

Citation

Cao, Yiyun, et al. "Isoflurane Anesthesia Results in Reversible Ultrastructure and Occludin Tight Junction Protein Expression Changes in Hippocampal Blood-brain Barrier in Aged Rats." Neuroscience Letters, vol. 587, 2015, pp. 51-6.
Cao Y, Ni C, Li Z, et al. Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats. Neurosci Lett. 2015;587:51-6.
Cao, Y., Ni, C., Li, Z., Li, L., Liu, Y., Wang, C., ... Guo, X. (2015). Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats. Neuroscience Letters, 587, pp. 51-6. doi:10.1016/j.neulet.2014.12.018.
Cao Y, et al. Isoflurane Anesthesia Results in Reversible Ultrastructure and Occludin Tight Junction Protein Expression Changes in Hippocampal Blood-brain Barrier in Aged Rats. Neurosci Lett. 2015 Feb 5;587:51-6. PubMed PMID: 25524410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoflurane anesthesia results in reversible ultrastructure and occludin tight junction protein expression changes in hippocampal blood-brain barrier in aged rats. AU - Cao,Yiyun, AU - Ni,Cheng, AU - Li,Zhengqian, AU - Li,Lunxu, AU - Liu,Yajie, AU - Wang,Chunyi, AU - Zhong,Yanfeng, AU - Cui,Dehua, AU - Guo,Xiangyang, Y1 - 2014/12/15/ PY - 2014/09/19/received PY - 2014/11/18/revised PY - 2014/12/11/accepted PY - 2014/12/20/entrez PY - 2014/12/20/pubmed PY - 2015/8/11/medline KW - Blood–brain barrier KW - Hippocampus KW - Postoperative cognitive dysfunction KW - Tight junction protein SP - 51 EP - 6 JF - Neuroscience letters JO - Neurosci. Lett. VL - 587 N2 - The underlying mechanism of isoflurane-induced cognitive dysfunction in older individuals is unknown. In this study, the effects of isoflurane exposure on the hippocampal blood-brain barrier (BBB) in aged rats were investigated because it was previously shown that BBB disruption involves in cognitive dysfunction. Twenty-month-old rats randomly received 1.5% isoflurane or vehicle gas as control. Hippocampal BBB ultrastructure was analyzed by transmission electron microscopy and expression of tight junction proteins was measured by western blot analysis. BBB permeability was detected with sodium fluorescein extravasation and further confirmed by immunoglobulin G immunohistochemistry. Spatial learning and memory were assessed by the Morris water maze test. Isoflurane anesthesia resulted in reversible time-dependent BBB ultrastructure morphological damage and significant decreases in expression of the tight junction proteins occludin, which contributed to sodium fluorescein and IgG leakage. Rats with isoflurane exposure also showed significant cognitive deficits in the Morris water maze test. This in vivo data indicate that occludin down-regulation may be one of the mediators of isoflurane-induced hippocampus BBB disruption, and may contribute to hippocampus-dependent cognitive impairment after isoflurane exposure in aged rats. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/25524410/Isoflurane_anesthesia_results_in_reversible_ultrastructure_and_occludin_tight_junction_protein_expression_changes_in_hippocampal_blood_brain_barrier_in_aged_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(14)00954-9 DB - PRIME DP - Unbound Medicine ER -