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Cerebellar vermis H2 receptors mediate fear memory consolidation in mice.
Neurosci Lett. 2015 Feb 05; 587:57-61.NL

Abstract

Histaminergic fibers are present in the molecular and granular layers of the cerebellum and have a high density in the vermis and flocullus. Evidence supports that the cerebellar histaminergic system is involved in memory consolidation. Our recent study showed that histamine injections facilitate the retention of an inhibitory avoidance task, which was abolished by pretreatment with an H2 receptor antagonist. In the present study, we investigated the effects of intracerebellar post training injections of H1 and H2 receptor antagonists as well as the selective H2 receptor agonist on fear memory consolidation. The cerebellar vermi of male mice were implanted with guide cannulae, and after three days of recovery, the inhibitory avoidance test was performed. Immediately after a training session, animals received a microinjection of the following histaminergic drugs: experiment 1, saline or chlorpheniramine (0.016, 0.052 or 0.16 nmol); experiment 2, saline or ranitidine (0.57, 2.85 or 5.07 nmol); and experiment 3, saline or dimaprit (1, 2 or 4 nmol). Twenty-four hours later, a retention test was performed. The data were analyzed using one-way analysis of variance (ANOVA) and Duncan's tests. Animals microinjected with chlorpheniramine did not show any behavioral effects at the doses that we used. Intra-cerebellar injection of the H2 receptor antagonist ranitidine inhibited, while the selective H2 receptor agonist dimaprit facilitated, memory consolidation, suggesting that H2 receptors mediate memory consolidation in the inhibitory avoidance task in mice.

Authors+Show Affiliations

Laboratory of Neuroscience, Physiotherapy Department, Center of Biological Sciences and Health, Federal University of Sao Carlos, 13565-905, Sao Carlos, Brazil. Electronic address: acgianlorenco@yahoo.com.br.Laboratory of Neuroscience, Physiotherapy Department, Center of Biological Sciences and Health, Federal University of Sao Carlos, 13565-905, Sao Carlos, Brazil. Electronic address: alineriboldi@yahoo.com.br.Laboratory of Neuroscience, Physiotherapy Department, Center of Biological Sciences and Health, Federal University of Sao Carlos, 13565-905, Sao Carlos, Brazil. Electronic address: bruna_chii@hotmail.com.Laboratory of Neuroscience, Physiotherapy Department, Center of Biological Sciences and Health, Federal University of Sao Carlos, 13565-905, Sao Carlos, Brazil. Electronic address: mattioli@ufscar.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25524412

Citation

Gianlorenço, A C L., et al. "Cerebellar Vermis H2 Receptors Mediate Fear Memory Consolidation in Mice." Neuroscience Letters, vol. 587, 2015, pp. 57-61.
Gianlorenço AC, Riboldi AM, Silva-Marques B, et al. Cerebellar vermis H2 receptors mediate fear memory consolidation in mice. Neurosci Lett. 2015;587:57-61.
Gianlorenço, A. C., Riboldi, A. M., Silva-Marques, B., & Mattioli, R. (2015). Cerebellar vermis H2 receptors mediate fear memory consolidation in mice. Neuroscience Letters, 587, 57-61. https://doi.org/10.1016/j.neulet.2014.12.017
Gianlorenço AC, et al. Cerebellar Vermis H2 Receptors Mediate Fear Memory Consolidation in Mice. Neurosci Lett. 2015 Feb 5;587:57-61. PubMed PMID: 25524412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cerebellar vermis H2 receptors mediate fear memory consolidation in mice. AU - Gianlorenço,A C L, AU - Riboldi,A M, AU - Silva-Marques,B, AU - Mattioli,R, Y1 - 2014/12/15/ PY - 2014/10/14/received PY - 2014/12/02/revised PY - 2014/12/11/accepted PY - 2014/12/20/entrez PY - 2014/12/20/pubmed PY - 2015/8/11/medline KW - Cerebellar vermis KW - H(2) receptors KW - Histaminergic system KW - Inhibitory avoidance KW - Memory consolidation KW - Mice SP - 57 EP - 61 JF - Neuroscience letters JO - Neurosci Lett VL - 587 N2 - Histaminergic fibers are present in the molecular and granular layers of the cerebellum and have a high density in the vermis and flocullus. Evidence supports that the cerebellar histaminergic system is involved in memory consolidation. Our recent study showed that histamine injections facilitate the retention of an inhibitory avoidance task, which was abolished by pretreatment with an H2 receptor antagonist. In the present study, we investigated the effects of intracerebellar post training injections of H1 and H2 receptor antagonists as well as the selective H2 receptor agonist on fear memory consolidation. The cerebellar vermi of male mice were implanted with guide cannulae, and after three days of recovery, the inhibitory avoidance test was performed. Immediately after a training session, animals received a microinjection of the following histaminergic drugs: experiment 1, saline or chlorpheniramine (0.016, 0.052 or 0.16 nmol); experiment 2, saline or ranitidine (0.57, 2.85 or 5.07 nmol); and experiment 3, saline or dimaprit (1, 2 or 4 nmol). Twenty-four hours later, a retention test was performed. The data were analyzed using one-way analysis of variance (ANOVA) and Duncan's tests. Animals microinjected with chlorpheniramine did not show any behavioral effects at the doses that we used. Intra-cerebellar injection of the H2 receptor antagonist ranitidine inhibited, while the selective H2 receptor agonist dimaprit facilitated, memory consolidation, suggesting that H2 receptors mediate memory consolidation in the inhibitory avoidance task in mice. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/25524412/Cerebellar_vermis_H2_receptors_mediate_fear_memory_consolidation_in_mice_ DB - PRIME DP - Unbound Medicine ER -