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Detection of ALK rearrangements in malignant pleural effusion cell blocks from patients with advanced non-small cell lung cancer: a comparison of Ventana immunohistochemistry and fluorescence in situ hybridization.
Cancer Cytopathol 2015; 123(2):117-22CC

Abstract

BACKGROUND

Surgical resections or tumor biopsies are often not available for patients with late-stage non-small cell lung cancer (NSCLC). Cytological specimens, such as malignant pleural effusion (MPE) cell blocks, are critical for molecular testing. Currently, diagnostic methods to identify anaplastic lymphoma kinase (ALK) rearrangements include fluorescence in situ hybridization (FISH), real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and immunohistochemistry (IHC). In the current study, the authors compared Ventana ALK IHC assays and ALK FISH to detect ALK rearrangements in MPE cell blocks from patients with advanced NSCLC.

METHODS

The ALK IHC assay and ALK FISH were performed on 63 MPE cell blocks. RT-PCR analysis was performed as additional validation in cases in which a discrepancy was observed between the IHC assay and FISH results.

RESULTS

The Ventana ALK IHC assay was found to be informative for all 63 samples, and 8 cases were positive. Fifty-eight cases were interpretable for FISH detection, and 6 were positive. The concordance between IHC and FISH was 100% among the 58 cases. Of the 5 uninterpretable ALK FISH cases, 2 cases and 3 cases, respectively, were ALK IHC positive and negative. One of the 2 ALK IHC-positive cases also demonstrated a positive result in the RT-PCR assay and the patient benefited from crizotinib treatment.

CONCLUSIONS

MPE cell blocks can be used successfully for the detection of ALK rearrangement when tumor tissue is not available. The Ventana ALK IHC assay is an effective screening method for ALK rearrangement in MPE cell blocks from patients with advanced NSCLC, demonstrating high agreement with FISH results.

Authors+Show Affiliations

Department of Pathology, West China Hospital of Sichuan University, Chengdu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25529354

Citation

Wang, Weiya, et al. "Detection of ALK Rearrangements in Malignant Pleural Effusion Cell Blocks From Patients With Advanced Non-small Cell Lung Cancer: a Comparison of Ventana Immunohistochemistry and Fluorescence in Situ Hybridization." Cancer Cytopathology, vol. 123, no. 2, 2015, pp. 117-22.
Wang W, Tang Y, Li J, et al. Detection of ALK rearrangements in malignant pleural effusion cell blocks from patients with advanced non-small cell lung cancer: a comparison of Ventana immunohistochemistry and fluorescence in situ hybridization. Cancer Cytopathol. 2015;123(2):117-22.
Wang, W., Tang, Y., Li, J., Jiang, L., Jiang, Y., & Su, X. (2015). Detection of ALK rearrangements in malignant pleural effusion cell blocks from patients with advanced non-small cell lung cancer: a comparison of Ventana immunohistochemistry and fluorescence in situ hybridization. Cancer Cytopathology, 123(2), pp. 117-22. doi:10.1002/cncy.21510.
Wang W, et al. Detection of ALK Rearrangements in Malignant Pleural Effusion Cell Blocks From Patients With Advanced Non-small Cell Lung Cancer: a Comparison of Ventana Immunohistochemistry and Fluorescence in Situ Hybridization. Cancer Cytopathol. 2015;123(2):117-22. PubMed PMID: 25529354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of ALK rearrangements in malignant pleural effusion cell blocks from patients with advanced non-small cell lung cancer: a comparison of Ventana immunohistochemistry and fluorescence in situ hybridization. AU - Wang,Weiya, AU - Tang,Yuan, AU - Li,Jinnan, AU - Jiang,Lili, AU - Jiang,Yong, AU - Su,Xueying, Y1 - 2014/12/19/ PY - 2014/09/10/received PY - 2014/11/20/revised PY - 2014/12/01/accepted PY - 2014/12/23/entrez PY - 2014/12/23/pubmed PY - 2015/4/10/medline KW - Ventana immunohistochemistry (IHC) assay KW - anaplastic lymphoma kinase (ALK) KW - cell block KW - fluorescence KW - fluorescence in situ hybridization (FISH) KW - malignant pleural effusion KW - non-small cell lung cancer SP - 117 EP - 22 JF - Cancer cytopathology JO - Cancer Cytopathol VL - 123 IS - 2 N2 - BACKGROUND: Surgical resections or tumor biopsies are often not available for patients with late-stage non-small cell lung cancer (NSCLC). Cytological specimens, such as malignant pleural effusion (MPE) cell blocks, are critical for molecular testing. Currently, diagnostic methods to identify anaplastic lymphoma kinase (ALK) rearrangements include fluorescence in situ hybridization (FISH), real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and immunohistochemistry (IHC). In the current study, the authors compared Ventana ALK IHC assays and ALK FISH to detect ALK rearrangements in MPE cell blocks from patients with advanced NSCLC. METHODS: The ALK IHC assay and ALK FISH were performed on 63 MPE cell blocks. RT-PCR analysis was performed as additional validation in cases in which a discrepancy was observed between the IHC assay and FISH results. RESULTS: The Ventana ALK IHC assay was found to be informative for all 63 samples, and 8 cases were positive. Fifty-eight cases were interpretable for FISH detection, and 6 were positive. The concordance between IHC and FISH was 100% among the 58 cases. Of the 5 uninterpretable ALK FISH cases, 2 cases and 3 cases, respectively, were ALK IHC positive and negative. One of the 2 ALK IHC-positive cases also demonstrated a positive result in the RT-PCR assay and the patient benefited from crizotinib treatment. CONCLUSIONS: MPE cell blocks can be used successfully for the detection of ALK rearrangement when tumor tissue is not available. The Ventana ALK IHC assay is an effective screening method for ALK rearrangement in MPE cell blocks from patients with advanced NSCLC, demonstrating high agreement with FISH results. SN - 1934-6638 UR - https://www.unboundmedicine.com/medline/citation/25529354/Detection_of_ALK_rearrangements_in_malignant_pleural_effusion_cell_blocks_from_patients_with_advanced_non_small_cell_lung_cancer:_a_comparison_of_Ventana_immunohistochemistry_and_fluorescence_in_situ_hybridization_ L2 - https://doi.org/10.1002/cncy.21510 DB - PRIME DP - Unbound Medicine ER -