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Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus.
Fish Shellfish Immunol. 2015 Mar; 43(1):60-6.FS

Abstract

A feeding trial was conducted to investigate the effects of dietary administration of probiotic with Bacillus subtilis, Aspergillus oryzae and Saccharomyces cerevisiae on growth, innate immune response, Hemato-immunological parameters and disease resistance of Nile tilapia, Oreochromis niloticus. Animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: Control, non-supplemented diet; 5 g kg(-1) probiotic mixture (B. subtilis 1.5 × 10(9) CFU g(-1), S. cerevisiae 10(9) CFU g(-1) and A. oryzae 2 × 10(9) CFU g(-1)); and 10 g kg(-1) probiotic mixture (B. subtilis 3.0 × 10(9) CFU g(-1), S. cerevisiae 2.0 × 10(9) CFU g(-1) and A. oryzae 4.0 × 10(9) CFU g(-1)). The respiratory burst activity, white blood cells and hematological parameters were evaluated after four, five and six weeks of feeding. At the end of the growth trial, fish were sampled for intestinal microbiology and challenged by intraperitoneal injection of LD50 concentration of Aeromonas hydrophila and Streptococcus iniae. Mortality was recorded for the following 3 weeks. Results showed that administration of the probiotic had no significant effect on the growth rates of Nile tilapias, although the fish fed probiotics had better feed conversion. Respiratory burst activity, erythrocyte fragility and levels of white blood cells were significantly improved in tilapias fed diet supplemented with probiotic levels (P < 0.05), which may exhibit up-regulating effects on tilapia immune parameters. The cumulative mortality after A. hydrophila and S. iniae challenge decreased in tilapias fed with probiotic (P < 0.05). The present study demonstrated the potential of B. subtilis, S. cerevisiae and A. oryzae combined as beneficial dietary probiotic in juvenile O. niloticus.

Authors+Show Affiliations

Embrapa - Brazilian Agricultural Research Corporation, Fisheries and Aquaculture, 104 Sul, LO 1 Avenue, 34, Palmas, TO 77020-020, Brazil. Electronic address: marina.iwashita@embrapa.br.Fisheries Institute, 455 Matarazzo Avenue, Parque da Água Branca, São Paulo, SP 05001-900, Brazil.Auburn University, School of Fisheries, Aquaculture and Aquatic Sciences, 203 Swingle Hall, Auburn, AL 36830, USA.Auburn University, School of Fisheries, Aquaculture and Aquatic Sciences, 203 Swingle Hall, Auburn, AL 36830, USA.Fisheries Institute, 455 Matarazzo Avenue, Parque da Água Branca, São Paulo, SP 05001-900, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25530581

Citation

Iwashita, Marina Keiko P., et al. "Dietary Supplementation With Bacillus Subtilis, Saccharomyces Cerevisiae and Aspergillus Oryzae Enhance Immunity and Disease Resistance Against Aeromonas Hydrophila and Streptococcus Iniae Infection in Juvenile Tilapia Oreochromis Niloticus." Fish & Shellfish Immunology, vol. 43, no. 1, 2015, pp. 60-6.
Iwashita MK, Nakandakare IB, Terhune JS, et al. Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus. Fish Shellfish Immunol. 2015;43(1):60-6.
Iwashita, M. K., Nakandakare, I. B., Terhune, J. S., Wood, T., & Ranzani-Paiva, M. J. (2015). Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus. Fish & Shellfish Immunology, 43(1), 60-6. https://doi.org/10.1016/j.fsi.2014.12.008
Iwashita MK, et al. Dietary Supplementation With Bacillus Subtilis, Saccharomyces Cerevisiae and Aspergillus Oryzae Enhance Immunity and Disease Resistance Against Aeromonas Hydrophila and Streptococcus Iniae Infection in Juvenile Tilapia Oreochromis Niloticus. Fish Shellfish Immunol. 2015;43(1):60-6. PubMed PMID: 25530581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus. AU - Iwashita,Marina Keiko P, AU - Nakandakare,Ivan B, AU - Terhune,Jeffery S, AU - Wood,Theresa, AU - Ranzani-Paiva,Maria José T, Y1 - 2014/12/19/ PY - 2014/06/03/received PY - 2014/12/08/revised PY - 2014/12/11/accepted PY - 2014/12/23/entrez PY - 2014/12/23/pubmed PY - 2015/10/2/medline KW - Bacterial challenge KW - Hemato-immunity KW - Innate immunity KW - Survival SP - 60 EP - 6 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 43 IS - 1 N2 - A feeding trial was conducted to investigate the effects of dietary administration of probiotic with Bacillus subtilis, Aspergillus oryzae and Saccharomyces cerevisiae on growth, innate immune response, Hemato-immunological parameters and disease resistance of Nile tilapia, Oreochromis niloticus. Animals were distributed in three equal groups, each of five replicates and received one of the following experimental diets for four weeks: Control, non-supplemented diet; 5 g kg(-1) probiotic mixture (B. subtilis 1.5 × 10(9) CFU g(-1), S. cerevisiae 10(9) CFU g(-1) and A. oryzae 2 × 10(9) CFU g(-1)); and 10 g kg(-1) probiotic mixture (B. subtilis 3.0 × 10(9) CFU g(-1), S. cerevisiae 2.0 × 10(9) CFU g(-1) and A. oryzae 4.0 × 10(9) CFU g(-1)). The respiratory burst activity, white blood cells and hematological parameters were evaluated after four, five and six weeks of feeding. At the end of the growth trial, fish were sampled for intestinal microbiology and challenged by intraperitoneal injection of LD50 concentration of Aeromonas hydrophila and Streptococcus iniae. Mortality was recorded for the following 3 weeks. Results showed that administration of the probiotic had no significant effect on the growth rates of Nile tilapias, although the fish fed probiotics had better feed conversion. Respiratory burst activity, erythrocyte fragility and levels of white blood cells were significantly improved in tilapias fed diet supplemented with probiotic levels (P < 0.05), which may exhibit up-regulating effects on tilapia immune parameters. The cumulative mortality after A. hydrophila and S. iniae challenge decreased in tilapias fed with probiotic (P < 0.05). The present study demonstrated the potential of B. subtilis, S. cerevisiae and A. oryzae combined as beneficial dietary probiotic in juvenile O. niloticus. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/25530581/Dietary_supplementation_with_Bacillus_subtilis_Saccharomyces_cerevisiae_and_Aspergillus_oryzae_enhance_immunity_and_disease_resistance_against_Aeromonas_hydrophila_and_Streptococcus_iniae_infection_in_juvenile_tilapia_Oreochromis_niloticus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(14)00479-3 DB - PRIME DP - Unbound Medicine ER -