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Up-regulation of intrarenal renin-agiotensin system contributes to renal damage in high-salt induced hypertension rats.
Kidney Blood Press Res. 2014; 39(6):526-35.KB

Abstract

BACKGROUND/AIMS

To investigate the change of intrarenal renin-agiotensin system (RAS) and its role in high-salt induced hypertension.

METHODS

Wistar rats were divided into normal-salt (NS), high-salt diet (HS) and high-salt diet with Losartan group (HS+L), for 6 weeks. Systolic blood pressure (SBP) was monitored. Blood and urine samples were collected every 2 weeks. Angiotensinogen (AGT) was measured by ELISA. AGT mRNA and protein were measured by real-time PCR and immunohistochemistry. Renin activity and angiotensin II (Ang II) were measured by radioimmunoassay.

RESULTS

HS versus NS group, SBP increased from 2(nd) week (P<0.05), urinary protein increased at 6(th) week (P<0.05). Although plasma renin, AGT and Ang II had no significant changes (P>0.05), renal cortex renin, AGT, and Ang II increased significantly in HS (P<0.05). In HS+L, Losartan failed to reduce SBP (P>0.05) but abolished the increase of proteinuria (P<0.01), renal cortex renin, AGT, Ang II and urinary AGT reduced (P<0.05) while plasma renin, AGT and Ang II enhanced (P<0.05) when compared with HS. Urinary AGT was positively correlated with renal AGT (r=0.592, P <0.01) and Ang II (r=0.726, P <0.01).

CONCLUSION

Inappropriate response of the renal RAS to a high salt diet may contribute to hypertension and renal damage, and urinary AGT could reflect intrarenal RAS activity.

Authors+Show Affiliations

Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25531334

Citation

Wu, Haiyan, et al. "Up-regulation of Intrarenal Renin-agiotensin System Contributes to Renal Damage in High-salt Induced Hypertension Rats." Kidney & Blood Pressure Research, vol. 39, no. 6, 2014, pp. 526-35.
Wu H, Liang Y, Zheng Y, et al. Up-regulation of intrarenal renin-agiotensin system contributes to renal damage in high-salt induced hypertension rats. Kidney Blood Press Res. 2014;39(6):526-35.
Wu, H., Liang, Y., Zheng, Y., Bai, Q., Zhuang, Z., A, L., Zheng, D., & Wang, Y. (2014). Up-regulation of intrarenal renin-agiotensin system contributes to renal damage in high-salt induced hypertension rats. Kidney & Blood Pressure Research, 39(6), 526-35. https://doi.org/10.1159/000368463
Wu H, et al. Up-regulation of Intrarenal Renin-agiotensin System Contributes to Renal Damage in High-salt Induced Hypertension Rats. Kidney Blood Press Res. 2014;39(6):526-35. PubMed PMID: 25531334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of intrarenal renin-agiotensin system contributes to renal damage in high-salt induced hypertension rats. AU - Wu,Haiyan, AU - Liang,Yaoxian, AU - Zheng,Yimu, AU - Bai,Qiong, AU - Zhuang,Zheng, AU - A,Lata, AU - Zheng,Danxia, AU - Wang,Yue, Y1 - 2014/11/29/ PY - 2014/10/17/accepted PY - 2014/12/23/entrez PY - 2014/12/23/pubmed PY - 2015/9/9/medline SP - 526 EP - 35 JF - Kidney & blood pressure research JO - Kidney Blood Press. Res. VL - 39 IS - 6 N2 - BACKGROUND/AIMS: To investigate the change of intrarenal renin-agiotensin system (RAS) and its role in high-salt induced hypertension. METHODS: Wistar rats were divided into normal-salt (NS), high-salt diet (HS) and high-salt diet with Losartan group (HS+L), for 6 weeks. Systolic blood pressure (SBP) was monitored. Blood and urine samples were collected every 2 weeks. Angiotensinogen (AGT) was measured by ELISA. AGT mRNA and protein were measured by real-time PCR and immunohistochemistry. Renin activity and angiotensin II (Ang II) were measured by radioimmunoassay. RESULTS: HS versus NS group, SBP increased from 2(nd) week (P<0.05), urinary protein increased at 6(th) week (P<0.05). Although plasma renin, AGT and Ang II had no significant changes (P>0.05), renal cortex renin, AGT, and Ang II increased significantly in HS (P<0.05). In HS+L, Losartan failed to reduce SBP (P>0.05) but abolished the increase of proteinuria (P<0.01), renal cortex renin, AGT, Ang II and urinary AGT reduced (P<0.05) while plasma renin, AGT and Ang II enhanced (P<0.05) when compared with HS. Urinary AGT was positively correlated with renal AGT (r=0.592, P <0.01) and Ang II (r=0.726, P <0.01). CONCLUSION: Inappropriate response of the renal RAS to a high salt diet may contribute to hypertension and renal damage, and urinary AGT could reflect intrarenal RAS activity. SN - 1423-0143 UR - https://www.unboundmedicine.com/medline/citation/25531334/Up_regulation_of_intrarenal_renin_agiotensin_system_contributes_to_renal_damage_in_high_salt_induced_hypertension_rats_ L2 - https://www.karger.com?DOI=10.1159/000368463 DB - PRIME DP - Unbound Medicine ER -