TY - JOUR T1 - Determination and pharmacokinetic study of gentiopicroside, geniposide, baicalin, and swertiamarin in Chinese herbal formulae after oral administration in rats by LC-MS/MS. AU - Lu,Chia-Ming, AU - Lin,Lie-Chwen, AU - Tsai,Tung-Hu, Y1 - 2014/12/22/ PY - 2014/11/02/received PY - 2014/12/01/revised PY - 2014/12/17/accepted PY - 2014/12/24/entrez PY - 2014/12/24/pubmed PY - 2015/8/8/medline SP - 21560 EP - 78 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 19 IS - 12 N2 - A sensitive and efficient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of gentiopicroside, geniposide, baicalin, and swertiamarin in rat plasma. To avoid the stress caused by restraint or anesthesia, a freely moving rat model was used to investigate the pharmacokinetics of herbal medicine after the administration of a traditional Chinese herbal prescription of Long-Dan-Xie-Gan-Tang (10 g/kg, p.o.). Analytes were separated by a C18 column with a gradient system of methanol-water containing 1 mM ammonium acetate with 0.1% formic acid. The linear ranges were 10-500 ng/mL for gentiopicroside, geniposide, and baicalin, and 5-250 ng/mL for swertiamarin in biological samples. The intra- and inter-day precision (relative standard deviation) ranged from 0.9% to 11.4% and 0.3% to 14.4%, respectively. The accuracy (relative error) was from -6.3% to 10.1% at all quality control levels. The analytical system provided adequate matrix effect and recovery with good precision and accuracy. The pharmacokinetic data demonstrated that the area under concentration-time curve (AUC) values of gentiopicroside, geniposide, baicalin, and swertiamarin were 1417 ± 83.8, 302 ± 25.8, 753 ± 86.2, and 2.5 ± 0.1 min µg/mL. The pharmacokinetic profiles provide constructive information for the dosage regimen of herbal medicine and also contribute to elucidate the absorption mechanism in herbal applications and pharmacological experiments. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/25532849/Determination_and_pharmacokinetic_study_of_gentiopicroside_geniposide_baicalin_and_swertiamarin_in_Chinese_herbal_formulae_after_oral_administration_in_rats_by_LC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -