Pharmacokinetics screening for multi-components absorbed in the rat plasma after oral administration of traditional Chinese medicine Flos Lonicerae Japonicae-Fructus Forsythiae herb couple by sequential negative and positive ionization ultra-high-performance liquid chromatography/tandem triple quadrupole mass spectrometric detection.
J Chromatogr A. 2015 Jan 09; 1376:84-97.JC

Abstract

The current study aims to investigate the pharmacokinetics of multi-components (caffeic acid, quinic acid, genistein, luteolin, quercetin, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, arctigenin, genistin, luteoloside, astragalin, hyperoside, isoquercitrin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, rutin, loganin, pinoresinol-β-d-glucoside, phillyrin, isoforsythoside, forsythoside A and forsythoside B) following oral administration of Flos Lonicerae Japonicae-Fructus Forsythiae herb couple in rats. A rapid and sensitive UPLC-ESI-MS/MS with sequential positive and negative ionization modes was developed to determine the 23 absorbed ingredients using one sample preparation combined with three chromatographic conditions in rat plasma. After mixing with internal standard (IS) (tinidazole and chloramphenicol), samples were pretreated by liquid-liquid extraction (LLE) with n-butyl alcohol/ethyl acetate (1:1, v/v). The separations for pinoresinol-β-d-glucoside, phillyrin, isoforsythoside, forsythoside A and forsythoside B were performed on an ACQUITY UPLC BEH C18 column (100mm×2.1mm, 1.7μm) with acetonitrile/methanol (4:1, v/v)-water as mobile phase. For analyzing quinic acid, an ACQUITY UPLC HSS T3 column (100mm×2.1mm, 1.8μm) was applied with acetonitrile/methanol (4:1, v/v)-0.01% formic acid as mobile phase after dilution up to 25-fold. The same column was applied to the other components with acetonitrile/methanol (4:1, v/v)-0.4% formic acid as mobile phase. The method validation results demonstrated that the proposed method was sensitive, specific and reliable, which was successfully applied to the pharmacokinetic study of the multi-components after oral administration of Flos Lonicerae Japonicae-Fructus Forsythiae herb couple.

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Authors+Show Affiliations

Zhou W

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China; Jiangsu Engineering Research Center for Efficient Delivery System of TCM, PR China; Nanjing Engineering Research Center for Industrialization of Chinese Medicine Pellets, PR China; Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.

Tam KY

Faculty of Health Sciences, University of Macau, Macau SAR, PR China.

Meng M

Waters Corporation, PR China.

Shan J

Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.

Wang S

Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.

Ju W

Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210093, PR China.

Cai B

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.

Di L

MeSH

Administration, OralAnimalsChromatography, High Pressure LiquidDrug CombinationsDrugs, Chinese HerbalForsythiaLiquid-Liquid ExtractionLoniceraMaleRats, Sprague-DawleyTandem Mass Spectrometry

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25533397

Citation

Zhou, Wei, et al. "Pharmacokinetics Screening for Multi-components Absorbed in the Rat Plasma After Oral Administration of Traditional Chinese Medicine Flos Lonicerae Japonicae-Fructus Forsythiae Herb Couple By Sequential Negative and Positive Ionization Ultra-high-performance Liquid Chromatography/tandem Triple Quadrupole Mass Spectrometric Detection." Journal of Chromatography. A, vol. 1376, 2015, pp. 84-97.

Zhou W, Tam KY, Meng M, et al. Pharmacokinetics screening for multi-components absorbed in the rat plasma after oral administration of traditional Chinese medicine Flos Lonicerae Japonicae-Fructus Forsythiae herb couple by sequential negative and positive ionization ultra-high-performance liquid chromatography/tandem triple quadrupole mass spectrometric detection. J Chromatogr A. 2015;1376:84-97.

Zhou, W., Tam, K. Y., Meng, M., Shan, J., Wang, S., Ju, W., Cai, B., & Di, L. (2015). Pharmacokinetics screening for multi-components absorbed in the rat plasma after oral administration of traditional Chinese medicine Flos Lonicerae Japonicae-Fructus Forsythiae herb couple by sequential negative and positive ionization ultra-high-performance liquid chromatography/tandem triple quadrupole mass spectrometric detection. Journal of Chromatography. A, 1376, 84-97. https://doi.org/10.1016/j.chroma.2014.12.018

Zhou W, et al. Pharmacokinetics Screening for Multi-components Absorbed in the Rat Plasma After Oral Administration of Traditional Chinese Medicine Flos Lonicerae Japonicae-Fructus Forsythiae Herb Couple By Sequential Negative and Positive Ionization Ultra-high-performance Liquid Chromatography/tandem Triple Quadrupole Mass Spectrometric Detection. J Chromatogr A. 2015 Jan 9;1376:84-97. PubMed PMID: 25533397.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Pharmacokinetics screening for multi-components absorbed in the rat plasma after oral administration of traditional Chinese medicine Flos Lonicerae Japonicae-Fructus Forsythiae herb couple by sequential negative and positive ionization ultra-high-performance liquid chromatography/tandem triple quadrupole mass spectrometric detection. AU - Zhou,Wei, AU - Tam,Kin Y, AU - Meng,Minxin, AU - Shan,Jinjun, AU - Wang,Shouchuan, AU - Ju,Wenzheng, AU - Cai,Baochang, AU - Di,Liuqing, Y1 - 2014/12/15/ PY - 2014/05/31/received PY - 2014/11/17/revised PY - 2014/12/06/accepted PY - 2014/12/24/entrez PY - 2014/12/24/pubmed PY - 2015/5/13/medline KW - Dilution technology KW - Flos Lonicerae Japonicae–Fructus Forsythiae herb couple KW - Liquid–liquid extraction KW - Matrix effect KW - Multi-compounds KW - Pharmacokinetics SP - 84 EP - 97 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1376 N2 - The current study aims to investigate the pharmacokinetics of multi-components (caffeic acid, quinic acid, genistein, luteolin, quercetin, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, arctigenin, genistin, luteoloside, astragalin, hyperoside, isoquercitrin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, rutin, loganin, pinoresinol-β-d-glucoside, phillyrin, isoforsythoside, forsythoside A and forsythoside B) following oral administration of Flos Lonicerae Japonicae-Fructus Forsythiae herb couple in rats. A rapid and sensitive UPLC-ESI-MS/MS with sequential positive and negative ionization modes was developed to determine the 23 absorbed ingredients using one sample preparation combined with three chromatographic conditions in rat plasma. After mixing with internal standard (IS) (tinidazole and chloramphenicol), samples were pretreated by liquid-liquid extraction (LLE) with n-butyl alcohol/ethyl acetate (1:1, v/v). The separations for pinoresinol-β-d-glucoside, phillyrin, isoforsythoside, forsythoside A and forsythoside B were performed on an ACQUITY UPLC BEH C18 column (100mm×2.1mm, 1.7μm) with acetonitrile/methanol (4:1, v/v)-water as mobile phase. For analyzing quinic acid, an ACQUITY UPLC HSS T3 column (100mm×2.1mm, 1.8μm) was applied with acetonitrile/methanol (4:1, v/v)-0.01% formic acid as mobile phase after dilution up to 25-fold. The same column was applied to the other components with acetonitrile/methanol (4:1, v/v)-0.4% formic acid as mobile phase. The method validation results demonstrated that the proposed method was sensitive, specific and reliable, which was successfully applied to the pharmacokinetic study of the multi-components after oral administration of Flos Lonicerae Japonicae-Fructus Forsythiae herb couple. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/25533397/Pharmacokinetics_screening_for_multi_components_absorbed_in_the_rat_plasma_after_oral_administration_of_traditional_Chinese_medicine_Flos_Lonicerae_Japonicae_Fructus_Forsythiae_herb_couple_by_sequential_negative_and_positive_ionization_ultra_high_performance_liquid_chromatography/tandem_triple_quadrupole_mass_spectrometric_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(14)01924-4 DB - PRIME DP - Unbound Medicine ER -

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