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High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014.
Influenza Other Respir Viruses. 2015 Mar; 9(2):59-63.IO

Abstract

OBJECTIVES

An unexpectedly high proportion of children were admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, during September and October, 2014. In light of the ongoing outbreak of enterovirus-D68 (EV-D68) in North America a real-time RT-PCR for screening of enterovirus and enterovirus D68 was established.

DESIGN

We developed a duplex real-time RT-PCR for rapid screening of enterovirus D68. The method target the 5' non-translated region (NTR) of the HEV genome at a location generally used for enterovirus detection.

SAMPLE

Nasopharyngeal samples (n = 354), from children <15 years of age, received for respiratory virus analysis in OUH during September 1st and October 31nd, 2014, were tested for enterovirus and screened for enterovirus D68.

MAIN OUTCOME MEASURES AND RESULTS

The duplex real-time RT-PCR method was an efficient tool for rapid screening for EV-D68 in respiratory specimens. Enterovirus was detected in 66 (22%) of 303 pediatric nasopharyngeal samples collected from children hospitalised with acute respiratory infection within the two-month period. Out of these, 33 (50%) were EV-D68. EV-D68 was associated with acute flaccid paralysis in one child.

CONCLUSIONS

An unexpectedly high proportion of children admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, were diagnosed with EV- D68 during September 1st and October 31nd, 2014. These results emphasise that greater vigilance is required throughout Europe as enteroviruses are cause of severe respiratory disease.

Authors+Show Affiliations

Department of Virology, Norwegian Institute of Public Health, Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25534826

Citation

Bragstad, Karoline, et al. "High Frequency of Enterovirus D68 in Children Hospitalised With Respiratory Illness in Norway, Autumn 2014." Influenza and Other Respiratory Viruses, vol. 9, no. 2, 2015, pp. 59-63.
Bragstad K, Jakobsen K, Rojahn AE, et al. High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014. Influenza Other Respir Viruses. 2015;9(2):59-63.
Bragstad, K., Jakobsen, K., Rojahn, A. E., Skram, M. K., Vainio, K., Holberg-Petersen, M., Hungnes, O., Dudman, S. G., & Kran, A. M. (2015). High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014. Influenza and Other Respiratory Viruses, 9(2), 59-63. https://doi.org/10.1111/irv.12300
Bragstad K, et al. High Frequency of Enterovirus D68 in Children Hospitalised With Respiratory Illness in Norway, Autumn 2014. Influenza Other Respir Viruses. 2015;9(2):59-63. PubMed PMID: 25534826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014. AU - Bragstad,Karoline, AU - Jakobsen,Kirsti, AU - Rojahn,Astrid E, AU - Skram,Marius K, AU - Vainio,Kirsti, AU - Holberg-Petersen,Mona, AU - Hungnes,Olav, AU - Dudman,Susanne G, AU - Kran,Anne-Marte B, Y1 - 2014/12/23/ PY - 2014/12/05/accepted PY - 2014/12/24/entrez PY - 2014/12/24/pubmed PY - 2015/8/14/medline KW - Acute flaccid paralysis KW - enterovirus D68 KW - picornavirus KW - respiratory infections KW - respiratory viruses SP - 59 EP - 63 JF - Influenza and other respiratory viruses JO - Influenza Other Respir Viruses VL - 9 IS - 2 N2 - OBJECTIVES: An unexpectedly high proportion of children were admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, during September and October, 2014. In light of the ongoing outbreak of enterovirus-D68 (EV-D68) in North America a real-time RT-PCR for screening of enterovirus and enterovirus D68 was established. DESIGN: We developed a duplex real-time RT-PCR for rapid screening of enterovirus D68. The method target the 5' non-translated region (NTR) of the HEV genome at a location generally used for enterovirus detection. SAMPLE: Nasopharyngeal samples (n = 354), from children <15 years of age, received for respiratory virus analysis in OUH during September 1st and October 31nd, 2014, were tested for enterovirus and screened for enterovirus D68. MAIN OUTCOME MEASURES AND RESULTS: The duplex real-time RT-PCR method was an efficient tool for rapid screening for EV-D68 in respiratory specimens. Enterovirus was detected in 66 (22%) of 303 pediatric nasopharyngeal samples collected from children hospitalised with acute respiratory infection within the two-month period. Out of these, 33 (50%) were EV-D68. EV-D68 was associated with acute flaccid paralysis in one child. CONCLUSIONS: An unexpectedly high proportion of children admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, were diagnosed with EV- D68 during September 1st and October 31nd, 2014. These results emphasise that greater vigilance is required throughout Europe as enteroviruses are cause of severe respiratory disease. SN - 1750-2659 UR - https://www.unboundmedicine.com/medline/citation/25534826/High_frequency_of_enterovirus_D68_in_children_hospitalised_with_respiratory_illness_in_Norway_autumn_2014_ L2 - https://doi.org/10.1111/irv.12300 DB - PRIME DP - Unbound Medicine ER -