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Lipidomic approach towards deciphering anandamide effects in rat decidual cell.
J Cell Physiol. 2015 Jul; 230(7):1549-57.JC

Abstract

Altered phospholipid (PL) metabolism has been associated with pregnancy disorders. Moreover, lipid molecules such as endocannabinoids (eCBs) and prostaglandins (PGs) are important mediators of reproductive events. In humans, abnormal decidualization has been linked with unexplained infertility, miscarriage and endometrial pathologies. Anandamide (AEA), the major eCB, induces apoptosis in rat decidual cells. In this study, the PL profile of rat decidual cells was characterized by a Mass spectrometry (MS) based lipidomic approach. Furthermore, we analyzed a possible correlation between changes in PL of rat decidual cells' membrane and AEA-induced apoptosis. We found an increase in phosphatidylserine and a reduction of cardiolipin and phophatidylinositol relative contents. In addition, we observed an increase in the content of alkyl(alkenyl) acylPL, plasmalogens, and of long chain fatty acids especially with high degrees of unsaturation, as well as an increase in lipid hydroperoxides in treated cells. These findings provide novel insights on deregulation of lipid metabolism by anandamide, which may display further implications in decidualization process.

Authors+Show Affiliations

UCIBIO, REQUIMTE, Laboratory of Biochemistry, Biological Sciences Department, Faculty of Pharmacy, University of Porto, Porto, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25535694

Citation

Almada, Marta, et al. "Lipidomic Approach Towards Deciphering Anandamide Effects in Rat Decidual Cell." Journal of Cellular Physiology, vol. 230, no. 7, 2015, pp. 1549-57.
Almada M, Domingues MR, Dória ML, et al. Lipidomic approach towards deciphering anandamide effects in rat decidual cell. J Cell Physiol. 2015;230(7):1549-57.
Almada, M., Domingues, M. R., Dória, M. L., Fonseca, B. M., Teixeira, N. A., & Correia-da-Silva, G. (2015). Lipidomic approach towards deciphering anandamide effects in rat decidual cell. Journal of Cellular Physiology, 230(7), 1549-57. https://doi.org/10.1002/jcp.24901
Almada M, et al. Lipidomic Approach Towards Deciphering Anandamide Effects in Rat Decidual Cell. J Cell Physiol. 2015;230(7):1549-57. PubMed PMID: 25535694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipidomic approach towards deciphering anandamide effects in rat decidual cell. AU - Almada,Marta, AU - Domingues,M Rosário, AU - Dória,M Luísa, AU - Fonseca,Bruno M, AU - Teixeira,Natércia A, AU - Correia-da-Silva,Georgina, PY - 2014/10/20/received PY - 2014/12/17/accepted PY - 2014/12/24/entrez PY - 2014/12/24/pubmed PY - 2015/6/3/medline SP - 1549 EP - 57 JF - Journal of cellular physiology JO - J Cell Physiol VL - 230 IS - 7 N2 - Altered phospholipid (PL) metabolism has been associated with pregnancy disorders. Moreover, lipid molecules such as endocannabinoids (eCBs) and prostaglandins (PGs) are important mediators of reproductive events. In humans, abnormal decidualization has been linked with unexplained infertility, miscarriage and endometrial pathologies. Anandamide (AEA), the major eCB, induces apoptosis in rat decidual cells. In this study, the PL profile of rat decidual cells was characterized by a Mass spectrometry (MS) based lipidomic approach. Furthermore, we analyzed a possible correlation between changes in PL of rat decidual cells' membrane and AEA-induced apoptosis. We found an increase in phosphatidylserine and a reduction of cardiolipin and phophatidylinositol relative contents. In addition, we observed an increase in the content of alkyl(alkenyl) acylPL, plasmalogens, and of long chain fatty acids especially with high degrees of unsaturation, as well as an increase in lipid hydroperoxides in treated cells. These findings provide novel insights on deregulation of lipid metabolism by anandamide, which may display further implications in decidualization process. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/25535694/Lipidomic_approach_towards_deciphering_anandamide_effects_in_rat_decidual_cell_ L2 - https://doi.org/10.1002/jcp.24901 DB - PRIME DP - Unbound Medicine ER -