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Effect of polyunsaturated fatty acids and their metabolites on bleomycin-induced cytotoxic action on human neuroblastoma cells in vitro.
PLoS One. 2014; 9(12):e114766.Plos

Abstract

In the present study, we noted that bleomycin induced growth inhibitory action was augmented by all the polyunsaturated fatty acids (PUFAs) tested on human neuroblastoma IMR-32 (0.5 × 10(4) cells/100 µl of IMR) cells (EPA > DHA > ALA = GLA = AA > DGLA = LA: ∼ 60, 40, 30, 10-20% respectively) at the maximum doses used. Of all the prostaglandins (PGE1, PGE2, PGF2α, and PGI2) and leukotrienes (LTD4 and LTE4) tested; PGE1, PGE2 and LTD4 inhibited the growth of IMR-32 cells to a significant degree at the highest doses used. Lipoxin A4 (LXA4), 19,20-dihydroxydocosapentaenoate (19, 20 DiHDPA) and 10(S),17(S)-dihydroxy-4Z,7Z,11E,13Z,15E,19Z-docosahexaenoic acid (protectin: 10(S),17(S)DiHDoHE), metabolites of DHA, significantly inhibited the growth of IMR-32 cells. Pre-treatment with AA, GLA, DGLA and EPA and simultaneous treatment with all PUFAs used in the study augmented growth inhibitory action of bleomycin. Surprisingly, both indomethacin and nordihydroguaiaretic acid (NDGA) at 60 and 20 µg/ml respectively enhanced the growth of IMR-32 cells even in the presence of bleomycin. AA enhanced oxidant stress in IMR-32 cells as evidenced by an increase in lipid peroxides, superoxide dismutase levels and glutathione peroxidase activity. These results suggest that PUFAs suppress growth of human neuroblastoma cells, augment growth inhibitory action of bleomycin by enhancing formation of lipid peroxides and altering the status of anti-oxidants and, in all probability, increase the formation of lipoxins, resolvins and protectins from their respective precursors that possess growth inhibitory actions.

Authors+Show Affiliations

Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering Campus, Madhurawada, Visakhapatnam-530048, Andhra Pradesh, India.Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering Campus, Madhurawada, Visakhapatnam-530048, Andhra Pradesh, India.Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering Campus, Madhurawada, Visakhapatnam-530048, Andhra Pradesh, India.Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering Campus, Madhurawada, Visakhapatnam-530048, Andhra Pradesh, India.Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering Campus, Madhurawada, Visakhapatnam-530048, Andhra Pradesh, India.UND Life Sciences, 2020 S 360th St, # K-202, Federal Way, Washington, 98003, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25536345

Citation

Polavarapu, Sailaja, et al. "Effect of Polyunsaturated Fatty Acids and Their Metabolites On Bleomycin-induced Cytotoxic Action On Human Neuroblastoma Cells in Vitro." PloS One, vol. 9, no. 12, 2014, pp. e114766.
Polavarapu S, Mani AM, Gundala NK, et al. Effect of polyunsaturated fatty acids and their metabolites on bleomycin-induced cytotoxic action on human neuroblastoma cells in vitro. PLoS One. 2014;9(12):e114766.
Polavarapu, S., Mani, A. M., Gundala, N. K., Hari, A. D., Bathina, S., & Das, U. N. (2014). Effect of polyunsaturated fatty acids and their metabolites on bleomycin-induced cytotoxic action on human neuroblastoma cells in vitro. PloS One, 9(12), e114766. https://doi.org/10.1371/journal.pone.0114766
Polavarapu S, et al. Effect of Polyunsaturated Fatty Acids and Their Metabolites On Bleomycin-induced Cytotoxic Action On Human Neuroblastoma Cells in Vitro. PLoS One. 2014;9(12):e114766. PubMed PMID: 25536345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of polyunsaturated fatty acids and their metabolites on bleomycin-induced cytotoxic action on human neuroblastoma cells in vitro. AU - Polavarapu,Sailaja, AU - Mani,Arul M, AU - Gundala,Naveen K V, AU - Hari,Anasuya D, AU - Bathina,Siresha, AU - Das,Undurti N, Y1 - 2014/12/23/ PY - 2014/07/22/received PY - 2014/11/13/accepted PY - 2014/12/24/entrez PY - 2014/12/24/pubmed PY - 2015/8/19/medline SP - e114766 EP - e114766 JF - PloS one JO - PLoS One VL - 9 IS - 12 N2 - In the present study, we noted that bleomycin induced growth inhibitory action was augmented by all the polyunsaturated fatty acids (PUFAs) tested on human neuroblastoma IMR-32 (0.5 × 10(4) cells/100 µl of IMR) cells (EPA > DHA > ALA = GLA = AA > DGLA = LA: ∼ 60, 40, 30, 10-20% respectively) at the maximum doses used. Of all the prostaglandins (PGE1, PGE2, PGF2α, and PGI2) and leukotrienes (LTD4 and LTE4) tested; PGE1, PGE2 and LTD4 inhibited the growth of IMR-32 cells to a significant degree at the highest doses used. Lipoxin A4 (LXA4), 19,20-dihydroxydocosapentaenoate (19, 20 DiHDPA) and 10(S),17(S)-dihydroxy-4Z,7Z,11E,13Z,15E,19Z-docosahexaenoic acid (protectin: 10(S),17(S)DiHDoHE), metabolites of DHA, significantly inhibited the growth of IMR-32 cells. Pre-treatment with AA, GLA, DGLA and EPA and simultaneous treatment with all PUFAs used in the study augmented growth inhibitory action of bleomycin. Surprisingly, both indomethacin and nordihydroguaiaretic acid (NDGA) at 60 and 20 µg/ml respectively enhanced the growth of IMR-32 cells even in the presence of bleomycin. AA enhanced oxidant stress in IMR-32 cells as evidenced by an increase in lipid peroxides, superoxide dismutase levels and glutathione peroxidase activity. These results suggest that PUFAs suppress growth of human neuroblastoma cells, augment growth inhibitory action of bleomycin by enhancing formation of lipid peroxides and altering the status of anti-oxidants and, in all probability, increase the formation of lipoxins, resolvins and protectins from their respective precursors that possess growth inhibitory actions. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25536345/Effect_of_polyunsaturated_fatty_acids_and_their_metabolites_on_bleomycin_induced_cytotoxic_action_on_human_neuroblastoma_cells_in_vitro_ L2 - https://dx.plos.org/10.1371/journal.pone.0114766 DB - PRIME DP - Unbound Medicine ER -