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[Comparison of the expression profiles of cell death factors in articular cartilage between Kashin-Beck disease and osteoarthritis].
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Dec; 34(12):1785-9.NF

Abstract

OBJECTIVE

To compare the expressions of programmed cell death 5 (PDCD5) and early growth response protein-1 (EGR-1) in the articular cartilage between Kashin-Beck disease (KBD) and primary osteoarthritis and the roles of these factors in KBD cartilage.

METHODS

Cartilage specimens were collected from 10 confirmed KBD patients, 15 osteoarthritic patients and 6 healthy subjects. The expression levels of PDCD5 and EGR-1 in the cartilage were detected by immunohistochemistry staining, and the positive chondrocyte counts were recorded in the different layers of KBD and OA cartilages.

RESULTS

The KBD cartilages contained a significantly higher percentage of PDCD5-positive chondrocytes in the middle layer [(41.35 ± 2.97)%] than OA cartilages [(26.48 ± 2.04)%, P=0.001] and normal cartilages [(19.02 ± 1.88)%, P=0.000] with also obvious PDCD5 over-expression in the deeper layer compared to OA (P=0.000) and normal cartilages (P=0.029), but PDCD5 expression in the superficial layer of the cartilages showed no significant difference among the 3 groups(P>0.05). The average EGR-1 positivity rate in the superficial layer of the cartilage was significantly higher in KBD patients than in OA patients (P=0.000) and healthy controls (P=0.000), but in the middle layer, its positivity rate in KBD patients was higher than that in the normal control (P=0.017) but lower than that of OA cartilage (P=0.002); EGR-1 expression in the deeper layer was comparable in KBD and OA cartilages but both was higher than that in normal cartilages. PDCD5 and EGR-1 expressions were not correlated in either KBD or normal cartilages, but were positively correlated in the superficial layer of OA cartilages.

CONCLUSIONS

KBD cartilages show a significantly increased PDCD5 expression in the deeper layer and enhanced EGR-1 expression in both superficial and deeper layers, suggesting the involvement of PDCD5 and EGR-1 in the pathogenesis of KBD.

Authors+Show Affiliations

College of Public Health, Health Science Center, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace elements and Endemic Diseases, Ministry of Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.E-mail: wushixun313@stu.xjtu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25537903

Citation

Wu, Shixun, et al. "[Comparison of the Expression Profiles of Cell Death Factors in Articular Cartilage Between Kashin-Beck Disease and Osteoarthritis]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 34, no. 12, 2014, pp. 1785-9.
Wu S, Guo X, Zhang F, et al. [Comparison of the expression profiles of cell death factors in articular cartilage between Kashin-Beck disease and osteoarthritis]. Nan Fang Yi Ke Da Xue Xue Bao. 2014;34(12):1785-9.
Wu, S., Guo, X., Zhang, F., Zheng, J., & Zhang, Z. (2014). [Comparison of the expression profiles of cell death factors in articular cartilage between Kashin-Beck disease and osteoarthritis]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 34(12), 1785-9.
Wu S, et al. [Comparison of the Expression Profiles of Cell Death Factors in Articular Cartilage Between Kashin-Beck Disease and Osteoarthritis]. Nan Fang Yi Ke Da Xue Xue Bao. 2014;34(12):1785-9. PubMed PMID: 25537903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison of the expression profiles of cell death factors in articular cartilage between Kashin-Beck disease and osteoarthritis]. AU - Wu,Shixun, AU - Guo,Xiong, AU - Zhang,Feng, AU - Zheng,Jingjing, AU - Zhang,Zengtie, PY - 2014/12/25/entrez PY - 2014/12/30/pubmed PY - 2015/9/14/medline SP - 1785 EP - 9 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 34 IS - 12 N2 - OBJECTIVE: To compare the expressions of programmed cell death 5 (PDCD5) and early growth response protein-1 (EGR-1) in the articular cartilage between Kashin-Beck disease (KBD) and primary osteoarthritis and the roles of these factors in KBD cartilage. METHODS: Cartilage specimens were collected from 10 confirmed KBD patients, 15 osteoarthritic patients and 6 healthy subjects. The expression levels of PDCD5 and EGR-1 in the cartilage were detected by immunohistochemistry staining, and the positive chondrocyte counts were recorded in the different layers of KBD and OA cartilages. RESULTS: The KBD cartilages contained a significantly higher percentage of PDCD5-positive chondrocytes in the middle layer [(41.35 ± 2.97)%] than OA cartilages [(26.48 ± 2.04)%, P=0.001] and normal cartilages [(19.02 ± 1.88)%, P=0.000] with also obvious PDCD5 over-expression in the deeper layer compared to OA (P=0.000) and normal cartilages (P=0.029), but PDCD5 expression in the superficial layer of the cartilages showed no significant difference among the 3 groups(P>0.05). The average EGR-1 positivity rate in the superficial layer of the cartilage was significantly higher in KBD patients than in OA patients (P=0.000) and healthy controls (P=0.000), but in the middle layer, its positivity rate in KBD patients was higher than that in the normal control (P=0.017) but lower than that of OA cartilage (P=0.002); EGR-1 expression in the deeper layer was comparable in KBD and OA cartilages but both was higher than that in normal cartilages. PDCD5 and EGR-1 expressions were not correlated in either KBD or normal cartilages, but were positively correlated in the superficial layer of OA cartilages. CONCLUSIONS: KBD cartilages show a significantly increased PDCD5 expression in the deeper layer and enhanced EGR-1 expression in both superficial and deeper layers, suggesting the involvement of PDCD5 and EGR-1 in the pathogenesis of KBD. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/25537903/[Comparison_of_the_expression_profiles_of_cell_death_factors_in_articular_cartilage_between_Kashin_Beck_disease_and_osteoarthritis]_ L2 - https://medlineplus.gov/osteoarthritis.html DB - PRIME DP - Unbound Medicine ER -