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In vitro colonic catabolism of orange juice (poly)phenols.
Mol Nutr Food Res. 2015 Mar; 59(3):465-75.MN

Abstract

SCOPE

The role of colonic microbiota in the breakdown of hesperetin, naringenin, and ferulic acid, compounds found as glycosides in orange juice, was investigated using an in vitro fermentation model.

METHODS AND RESULTS

Test compounds were incubated with human fecal slurries cultured under anaerobic conditions, and the production of phenolic acid catabolites were monitored by GC-MS and HPLC-MS(2) . Hesperetin was converted to 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid, 3-(3',4'-dihydroxyphenyl)propionic acid, and 3-(3'-hydroxyphenyl)propionic acid while 3-(phenyl)propionic acid was the major end product derived from naringenin. The data obtained are compared to our previously published data on urinary excretion of phenolic and aromatic acids after acute orange juice consumption (Pereira-Caro et al. Am. J. Clin. Nutr. 2014, 100, 1385-1391). Catabolism pathways are proposed for events occurring in the colon and those taking place postabsorption into the circulatory system with particular reference to the excretion of 3-(3'-hydroxy-4'-methoxyphenyl)hydracrylic acid, which is not formed in fecal incubations. Ferulic acid was also degraded by the colonic microflora being converted principally to 3-(3'-methoxy-4'-hydroxyphenyl)propionic acid, a phenolic acid that appears in urine after orange juice consumption.

CONCLUSION

The study provides novel information on the potential involvement of the colonic microbiota in the overall bioavailability of orange juice (poly)phenols through the production of phenylpropionic acids and subsequent hepatic conversions that lead to hippuric acid and its hydroxylated analogues.

Authors+Show Affiliations

Department of Technology, Postharvest and Food Industry, IFAPA-Alameda del Obispo, Córdoba, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25545994

Citation

Pereira-Caro, Gema, et al. "In Vitro Colonic Catabolism of Orange Juice (poly)phenols." Molecular Nutrition & Food Research, vol. 59, no. 3, 2015, pp. 465-75.
Pereira-Caro G, Borges G, Ky I, et al. In vitro colonic catabolism of orange juice (poly)phenols. Mol Nutr Food Res. 2015;59(3):465-75.
Pereira-Caro, G., Borges, G., Ky, I., Ribas, A., Calani, L., Del Rio, D., Clifford, M. N., Roberts, S. A., & Crozier, A. (2015). In vitro colonic catabolism of orange juice (poly)phenols. Molecular Nutrition & Food Research, 59(3), 465-75. https://doi.org/10.1002/mnfr.201400779
Pereira-Caro G, et al. In Vitro Colonic Catabolism of Orange Juice (poly)phenols. Mol Nutr Food Res. 2015;59(3):465-75. PubMed PMID: 25545994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro colonic catabolism of orange juice (poly)phenols. AU - Pereira-Caro,Gema, AU - Borges,Gina, AU - Ky,Isabelle, AU - Ribas,Aleix, AU - Calani,Luca, AU - Del Rio,Daniele, AU - Clifford,Michael N, AU - Roberts,Susan A, AU - Crozier,Alan, Y1 - 2015/01/22/ PY - 2014/10/28/received PY - 2014/12/11/revised PY - 2014/12/16/accepted PY - 2014/12/30/entrez PY - 2014/12/30/pubmed PY - 2016/4/26/medline KW - 3-(Phenyl)hydracrylic acids KW - 3-(Phenyl)propionic acids KW - Colonic catabolites KW - Ferulic acid KW - Flavanones KW - Human gut microflora KW - In vitro fecal fermentation SP - 465 EP - 75 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 59 IS - 3 N2 - SCOPE: The role of colonic microbiota in the breakdown of hesperetin, naringenin, and ferulic acid, compounds found as glycosides in orange juice, was investigated using an in vitro fermentation model. METHODS AND RESULTS: Test compounds were incubated with human fecal slurries cultured under anaerobic conditions, and the production of phenolic acid catabolites were monitored by GC-MS and HPLC-MS(2) . Hesperetin was converted to 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid, 3-(3',4'-dihydroxyphenyl)propionic acid, and 3-(3'-hydroxyphenyl)propionic acid while 3-(phenyl)propionic acid was the major end product derived from naringenin. The data obtained are compared to our previously published data on urinary excretion of phenolic and aromatic acids after acute orange juice consumption (Pereira-Caro et al. Am. J. Clin. Nutr. 2014, 100, 1385-1391). Catabolism pathways are proposed for events occurring in the colon and those taking place postabsorption into the circulatory system with particular reference to the excretion of 3-(3'-hydroxy-4'-methoxyphenyl)hydracrylic acid, which is not formed in fecal incubations. Ferulic acid was also degraded by the colonic microflora being converted principally to 3-(3'-methoxy-4'-hydroxyphenyl)propionic acid, a phenolic acid that appears in urine after orange juice consumption. CONCLUSION: The study provides novel information on the potential involvement of the colonic microbiota in the overall bioavailability of orange juice (poly)phenols through the production of phenylpropionic acids and subsequent hepatic conversions that lead to hippuric acid and its hydroxylated analogues. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/25545994/In_vitro_colonic_catabolism_of_orange_juice__poly_phenols_ L2 - https://doi.org/10.1002/mnfr.201400779 DB - PRIME DP - Unbound Medicine ER -