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Scolicidal effects of black cumin seed (Nigella sativa) essential oil on hydatid cysts.
Korean J Parasitol 2014; 52(6):653-9KJ

Abstract

Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models.

Authors+Show Affiliations

Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. ; Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. ; Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25548417

Citation

Mahmoudvand, Hossein, et al. "Scolicidal Effects of Black Cumin Seed (Nigella Sativa) Essential Oil On Hydatid Cysts." The Korean Journal of Parasitology, vol. 52, no. 6, 2014, pp. 653-9.
Mahmoudvand H, Dezaki ES, Kheirandish F, et al. Scolicidal effects of black cumin seed (Nigella sativa) essential oil on hydatid cysts. Korean J Parasitol. 2014;52(6):653-9.
Mahmoudvand, H., Dezaki, E. S., Kheirandish, F., Ezatpour, B., Jahanbakhsh, S., & Harandi, M. F. (2014). Scolicidal effects of black cumin seed (Nigella sativa) essential oil on hydatid cysts. The Korean Journal of Parasitology, 52(6), pp. 653-9. doi:10.3347/kjp.2014.52.6.653.
Mahmoudvand H, et al. Scolicidal Effects of Black Cumin Seed (Nigella Sativa) Essential Oil On Hydatid Cysts. Korean J Parasitol. 2014;52(6):653-9. PubMed PMID: 25548417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Scolicidal effects of black cumin seed (Nigella sativa) essential oil on hydatid cysts. AU - Mahmoudvand,Hossein, AU - Dezaki,Ebrahim Saedi, AU - Kheirandish,Farnaz, AU - Ezatpour,Behrouz, AU - Jahanbakhsh,Sareh, AU - Harandi,Majid Fasihi, Y1 - 2014/12/23/ PY - 2014/07/11/received PY - 2014/08/09/revised PY - 2014/08/26/accepted PY - 2014/12/31/entrez PY - 2014/12/31/pubmed PY - 2015/6/30/medline KW - Echinococcus granulosus KW - Ranunculaceae KW - cystic echinococcosis KW - thymoquinone SP - 653 EP - 9 JF - The Korean journal of parasitology JO - Korean J. Parasitol. VL - 52 IS - 6 N2 - Surgery remains the preferred treatment for hydatid cyst (cystic echinococcosis, CE). Various scolicidal agents have been used for inactivation of protoscolices during surgery, but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal effect of Nigella sativa (Ranunculaceae) essential oil and also its active principle, thymoquinone, against protoscolices of hydatid cysts. Protoscolices were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (0.01-10 mg/ml) and thymoquinone (0.125-1.0 mg/ml) were used for 5 to 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. Furthermore, the components of the N. sativa essential oil were identified by gas chromatography/mass spectroscopy (GC/MS). Our study revealed that the essential oil of N. sativa at the concentration of 10 mg/ml and its main component, thymoquinone, at the concentration of 1 mg/ml had potent scolicidal activities against protoscolices of Echinococcus granulosus after 10 min exposure. Moreover, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%), and longifolene (6.1%) were found to be the major components of N. sativa essential oil by GC/MS analysis. The results of this study indicated the potential of N. sativa as a natural source for production of a new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in in vivo models. SN - 1738-0006 UR - https://www.unboundmedicine.com/medline/citation/25548417/Scolicidal_effects_of_black_cumin_seed__Nigella_sativa__essential_oil_on_hydatid_cysts_ L2 - http://www.parasitol.or.kr/kjp/DOIx.php?id=10.3347/kjp.2014.52.6.653 DB - PRIME DP - Unbound Medicine ER -