Tags

Type your tag names separated by a space and hit enter

In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats.
BMC Anesthesiol. 2014; 14:117.BA

Abstract

BACKGROUND

Acute lung injury (ALI) is a serious disease with high incidence in ICU, and impaired mitochondria function plays a significant role in ALI. In this study, we examined the possible roles of exogenous hydrogen sulfide (H2S) in lung mitochondria regulation in ALI rats.

METHODS

The rat ALI model was induced by an intra-tongue vein Lipopolysaccharide (LPS) injection. We used sodium hydrosulphide (NaHS) as the H2S donor. We randomly divided 40 Sprague-Dawley rats into five groups: control, LPS injury, LPS + low-dose NaHS (0.78 mg • kg(-1)), LPS + middle-dose NaHS (1.56 mg • kg(-1)), and LPS + high-dose NaHS (3.12 mg • kg(-1)). Rats were killed 3 h after NaHS administration. We calculated a semi-quantitative histological index of lung injury assessments and measured the lung wet-to-dry weight ratio. We further analyzed serum for interleukin-1β levels using enzyme-linked immunosorbent assays. We observed lung mitochondria ultrastructures with an electron microscope. We examined oxidative stress markers in lung mitochondria and the mitochondrial swelling and activity. We analyzed lung mitochondria and cytosol Cyt-c protein expression using Western blotting.

RESULTS

Compared to the control group, the quantitative assessment score index, wet-to-dry weight ratios, and interleukin-1β content in the LPS injury group were significantly increased and the mitochondrial ultrastructure damaged. Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group. These effects were lessened by NaHS.

CONCLUSION

Exogenous H2S provided a protective effect against ALI by decreasing the mitochondrial lipid peroxidation level and protecting the cell structure in the LPS-induced rat models. Its regulatory effect on lung mitochondria is positively correlated with the dosage.

Authors+Show Affiliations

Department of Pharmacology, Hebei Medical University, 361 Zhongshan Eastern Road, Shijiazhuang, 050017 China ; Intensive Care Unit, Hebei General Hospital, 348 Heping Western Road, Shijiazhuang, 050051 China.Clinical research center, Hebei General Hospital, 348 Heping Western Road, Shijiazhuang, 050051 China.Department of Pharmacology, Children's Hospital of Hebei Province, 133 Jianhua Southern Avenue, Shijiazhuang, 050031 China.Department of Pharmacology, Hebei Centers for Disease Control and Prevention, 97 Huaian Eastern Road, Shijiazhuang, 050021 China.Department of chest surgery, Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, 050051 China.Department of Pharmacology, Hebei Centers for Disease Control and Prevention, 97 Huaian Eastern Road, Shijiazhuang, 050021 China.Department of Pharmacology, Hebei Centers for Disease Control and Prevention, 97 Huaian Eastern Road, Shijiazhuang, 050021 China.Department of Pharmacology, Hebei Medical University, 361 Zhongshan Eastern Road, Shijiazhuang, 050017 China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25550681

Citation

Du, Quansheng, et al. "In Vivo Study of the Effects of Exogenous Hydrogen Sulfide On Lung Mitochondria in Acute Lung Injury in Rats." BMC Anesthesiology, vol. 14, 2014, p. 117.
Du Q, Wang C, Zhang N, et al. In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats. BMC Anesthesiol. 2014;14:117.
Du, Q., Wang, C., Zhang, N., Li, G., Zhang, M., Li, L., Zhang, Q., & Zhang, J. (2014). In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats. BMC Anesthesiology, 14, 117. https://doi.org/10.1186/1471-2253-14-117
Du Q, et al. In Vivo Study of the Effects of Exogenous Hydrogen Sulfide On Lung Mitochondria in Acute Lung Injury in Rats. BMC Anesthesiol. 2014;14:117. PubMed PMID: 25550681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats. AU - Du,Quansheng, AU - Wang,Chao, AU - Zhang,Nan, AU - Li,Guofeng, AU - Zhang,Meng, AU - Li,Liping, AU - Zhang,Qingzeng, AU - Zhang,Jianxin, Y1 - 2014/12/15/ PY - 2014/05/14/received PY - 2014/12/11/accepted PY - 2015/1/1/entrez PY - 2015/1/1/pubmed PY - 2015/7/7/medline KW - Acute lung injury KW - Exogenous hydrogen sulfide KW - Lipopolysaccharide KW - Mitochondria KW - Mitochondrial lipid peroxidation SP - 117 EP - 117 JF - BMC anesthesiology JO - BMC Anesthesiol VL - 14 N2 - BACKGROUND: Acute lung injury (ALI) is a serious disease with high incidence in ICU, and impaired mitochondria function plays a significant role in ALI. In this study, we examined the possible roles of exogenous hydrogen sulfide (H2S) in lung mitochondria regulation in ALI rats. METHODS: The rat ALI model was induced by an intra-tongue vein Lipopolysaccharide (LPS) injection. We used sodium hydrosulphide (NaHS) as the H2S donor. We randomly divided 40 Sprague-Dawley rats into five groups: control, LPS injury, LPS + low-dose NaHS (0.78 mg • kg(-1)), LPS + middle-dose NaHS (1.56 mg • kg(-1)), and LPS + high-dose NaHS (3.12 mg • kg(-1)). Rats were killed 3 h after NaHS administration. We calculated a semi-quantitative histological index of lung injury assessments and measured the lung wet-to-dry weight ratio. We further analyzed serum for interleukin-1β levels using enzyme-linked immunosorbent assays. We observed lung mitochondria ultrastructures with an electron microscope. We examined oxidative stress markers in lung mitochondria and the mitochondrial swelling and activity. We analyzed lung mitochondria and cytosol Cyt-c protein expression using Western blotting. RESULTS: Compared to the control group, the quantitative assessment score index, wet-to-dry weight ratios, and interleukin-1β content in the LPS injury group were significantly increased and the mitochondrial ultrastructure damaged. Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group. These effects were lessened by NaHS. CONCLUSION: Exogenous H2S provided a protective effect against ALI by decreasing the mitochondrial lipid peroxidation level and protecting the cell structure in the LPS-induced rat models. Its regulatory effect on lung mitochondria is positively correlated with the dosage. SN - 1471-2253 UR - https://www.unboundmedicine.com/medline/citation/25550681/In_vivo_study_of_the_effects_of_exogenous_hydrogen_sulfide_on_lung_mitochondria_in_acute_lung_injury_in_rats_ L2 - https://bmcanesthesiol.biomedcentral.com/articles/10.1186/1471-2253-14-117 DB - PRIME DP - Unbound Medicine ER -