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Effects of carnosine, taurine, and betaine pretreatments on diethylnitrosamine-induced oxidative stress and tissue injury in rat liver.
Toxicol Ind Health 2016; 32(8):1405-1413TI

Abstract

Several chemicals such as N-diethylnitrosamine (DEN) promote hepatocellular cancer in rodents and induce hepatocyte injury. DEN affects the initiation stage of carcinogenesis together with enhanced cell proliferation accompanied by hepatocellular necrosis. DEN-induced hepatocellular necrosis is reported to be related to enhanced generation of reactive oxygen species. Carnosine (CAR), taurine (TAU), and betaine (BET) are known to have powerful antioxidant properties. We aimed to investigate the effects of CAR, TAU, and BET pretreatments on DEN-induced oxidative stress and liver injury in male rats. Rats were given CAR (2 g L-1 in drinking water), TAU (2.5% in chow), and BET (2.5% in chow) for 6 weeks and DEN (200 mg kg-1 intraperitoneally) was given 2 days before the end of this period. Serum alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and γ-glutamyl transferase activities were determined and a histopathologic evaluation was performed on the liver tissue. Oxidative stress was detected in the liver by measuring malondialdehyde, diene conjugate, protein carbonyl and nitrotyrosine levels, glutathione and glutathione peroxidase levels, and superoxide dismutase and glutathione transferase activities. Pretreatments with CAR, TAU, and BET decreased liver prooxidant status without remarkable changes in antioxidant parameters in DEN-treated rats. Pretreatments with TAU and BET, but not CAR, were also found to be effective to reduce liver damage in DEN-treated rats. In conclusion, TAU, BET, and possibly CAR may have an ameliorating effect on DEN-induced hepatic injury by reducing oxidative stress in rats.

Authors+Show Affiliations

Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.Department of Pathology, Oncology Institute, Istanbul University, Istanbul, Turkey.Department of Pathology, Oncology Institute, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey sdabbasoglu@yahoo.com.Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

25552536

Citation

Başaran-Küçükgergin, C, et al. "Effects of Carnosine, Taurine, and Betaine Pretreatments On Diethylnitrosamine-induced Oxidative Stress and Tissue Injury in Rat Liver." Toxicology and Industrial Health, vol. 32, no. 8, 2016, pp. 1405-1413.
Başaran-Küçükgergin C, Bingül I, Tekkeşin MS, et al. Effects of carnosine, taurine, and betaine pretreatments on diethylnitrosamine-induced oxidative stress and tissue injury in rat liver. Toxicol Ind Health. 2016;32(8):1405-1413.
Başaran-Küçükgergin, C., Bingül, I., Tekkeşin, M. S., Olgaç, V., Doğru-Abbasoğlu, S., & Uysal, M. (2016). Effects of carnosine, taurine, and betaine pretreatments on diethylnitrosamine-induced oxidative stress and tissue injury in rat liver. Toxicology and Industrial Health, 32(8), pp. 1405-1413.
Başaran-Küçükgergin C, et al. Effects of Carnosine, Taurine, and Betaine Pretreatments On Diethylnitrosamine-induced Oxidative Stress and Tissue Injury in Rat Liver. Toxicol Ind Health. 2016;32(8):1405-1413. PubMed PMID: 25552536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of carnosine, taurine, and betaine pretreatments on diethylnitrosamine-induced oxidative stress and tissue injury in rat liver. AU - Başaran-Küçükgergin,C, AU - Bingül,I, AU - Tekkeşin,M Soluk, AU - Olgaç,V, AU - Doğru-Abbasoğlu,S, AU - Uysal,M, Y1 - 2014/12/31/ PY - 2015/1/2/pubmed PY - 2017/4/18/medline PY - 2015/1/2/entrez KW - Hepatic injury KW - betaine KW - carnosine KW - diethylnitrosamine KW - oxidative stress KW - taurine SP - 1405 EP - 1413 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 32 IS - 8 N2 - Several chemicals such as N-diethylnitrosamine (DEN) promote hepatocellular cancer in rodents and induce hepatocyte injury. DEN affects the initiation stage of carcinogenesis together with enhanced cell proliferation accompanied by hepatocellular necrosis. DEN-induced hepatocellular necrosis is reported to be related to enhanced generation of reactive oxygen species. Carnosine (CAR), taurine (TAU), and betaine (BET) are known to have powerful antioxidant properties. We aimed to investigate the effects of CAR, TAU, and BET pretreatments on DEN-induced oxidative stress and liver injury in male rats. Rats were given CAR (2 g L-1 in drinking water), TAU (2.5% in chow), and BET (2.5% in chow) for 6 weeks and DEN (200 mg kg-1 intraperitoneally) was given 2 days before the end of this period. Serum alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and γ-glutamyl transferase activities were determined and a histopathologic evaluation was performed on the liver tissue. Oxidative stress was detected in the liver by measuring malondialdehyde, diene conjugate, protein carbonyl and nitrotyrosine levels, glutathione and glutathione peroxidase levels, and superoxide dismutase and glutathione transferase activities. Pretreatments with CAR, TAU, and BET decreased liver prooxidant status without remarkable changes in antioxidant parameters in DEN-treated rats. Pretreatments with TAU and BET, but not CAR, were also found to be effective to reduce liver damage in DEN-treated rats. In conclusion, TAU, BET, and possibly CAR may have an ameliorating effect on DEN-induced hepatic injury by reducing oxidative stress in rats. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/25552536/Effects_of_carnosine_taurine_and_betaine_pretreatments_on_diethylnitrosamine_induced_oxidative_stress_and_tissue_injury_in_rat_liver_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233714563432?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -