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Detection of enteroviruses in pediatric patients with aseptic meningitis.
Clin Neurol Neurosurg. 2015 Feb; 129:67-71.CN

Abstract

Aseptic meningitis is an acute viral infection of the central nervous system that occurs most frequently in infants and young children. This study was conducted on 100 pediatric patients with ages range from 1.5 months to 6 years. Cerebrospinal fluid (CSF) specimens were obtained with criteria of aseptic CNS infections as documented by pleocytosis, negative Gram stain and negative bacterial culture. Clinical and CSF findings of the affected children were analyzed and CSF specimens were submitted to viral culture and polymerase chain reaction (PCR) techniques to determine the enteroviral etiology. Fifty six percent patients had positive PCR results for the enteroviral genome, compared with 20% by virus culture. We can conclude that PCR is a rapid, reliable and sensitive diagnostic tool for the detection of enteroviral infections. A positive EV-PCR result may affect clinical decision making and may significantly alter the medical care offered to infected patients.

Authors+Show Affiliations

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Giza, Egypt.Pediatrics Department, National Research Center, Cairo, Egypt(1). Electronic address: ibra_n2005@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25553519

Citation

Shaker, Olfat G., and Nehal Abdelhamid. "Detection of Enteroviruses in Pediatric Patients With Aseptic Meningitis." Clinical Neurology and Neurosurgery, vol. 129, 2015, pp. 67-71.
Shaker OG, Abdelhamid N. Detection of enteroviruses in pediatric patients with aseptic meningitis. Clin Neurol Neurosurg. 2015;129:67-71.
Shaker, O. G., & Abdelhamid, N. (2015). Detection of enteroviruses in pediatric patients with aseptic meningitis. Clinical Neurology and Neurosurgery, 129, 67-71. https://doi.org/10.1016/j.clineuro.2014.12.004
Shaker OG, Abdelhamid N. Detection of Enteroviruses in Pediatric Patients With Aseptic Meningitis. Clin Neurol Neurosurg. 2015;129:67-71. PubMed PMID: 25553519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of enteroviruses in pediatric patients with aseptic meningitis. AU - Shaker,Olfat G, AU - Abdelhamid,Nehal, Y1 - 2014/12/19/ PY - 2014/02/17/received PY - 2014/09/29/revised PY - 2014/12/06/accepted PY - 2015/1/2/entrez PY - 2015/1/2/pubmed PY - 2015/10/7/medline KW - Aseptic meningitis KW - Enteroviruses KW - Pediatric patients SP - 67 EP - 71 JF - Clinical neurology and neurosurgery JO - Clin Neurol Neurosurg VL - 129 N2 - Aseptic meningitis is an acute viral infection of the central nervous system that occurs most frequently in infants and young children. This study was conducted on 100 pediatric patients with ages range from 1.5 months to 6 years. Cerebrospinal fluid (CSF) specimens were obtained with criteria of aseptic CNS infections as documented by pleocytosis, negative Gram stain and negative bacterial culture. Clinical and CSF findings of the affected children were analyzed and CSF specimens were submitted to viral culture and polymerase chain reaction (PCR) techniques to determine the enteroviral etiology. Fifty six percent patients had positive PCR results for the enteroviral genome, compared with 20% by virus culture. We can conclude that PCR is a rapid, reliable and sensitive diagnostic tool for the detection of enteroviral infections. A positive EV-PCR result may affect clinical decision making and may significantly alter the medical care offered to infected patients. SN - 1872-6968 UR - https://www.unboundmedicine.com/medline/citation/25553519/Detection_of_enteroviruses_in_pediatric_patients_with_aseptic_meningitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-8467(14)00478-8 DB - PRIME DP - Unbound Medicine ER -