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Identification of functional platelet-activating factor receptors in Raji lymphoblasts.
J Immunol. 1989 Dec 01; 143(11):3708-13.JI

Abstract

The binding and metabolism of platelet-activating factor (PAF) were characterized in Raji, a human Burkitt's lymphoma-derived cell line. Raji lymphoblasts readily metabolized PAF by deacetylation-reacylation at 37 degrees C, but not at 4 degrees C. Binding studies conducted at 4 degrees C demonstrated specific binding that reached saturation within 80 min. This binding was only partially reversible. Scatchard analysis of PAF binding data revealed a single class of PAF binding sites (17,800 +/- 3,600/cell) with a K of 2.3 +/- 0.3 nM. These high-affinity PAF binding sites were shown to be functional receptors, as 100 pM to 1 microM PAF increased free intracellular calcium in a dose-dependent manner. The dose of PAF necessary to achieve half maximal calcium mobilization response was 6.3 nM, which was in the range of the K for the receptor calculated from the binding studies. The structurally dissimilar PAF receptor antagonists CV-3988 and BN52021 inhibited the PAF-induced calcium changes at doses that competed with PAF binding. These studies provide the first evidence for a functional PAF receptor expressed on a lymphocyte cell line.

Authors+Show Affiliations

Department of Pharmacology, Ohio State University College of Medicine, Columbus 43210.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2555416

Citation

Travers, J B., et al. "Identification of Functional Platelet-activating Factor Receptors in Raji Lymphoblasts." Journal of Immunology (Baltimore, Md. : 1950), vol. 143, no. 11, 1989, pp. 3708-13.
Travers JB, Li Q, Kniss DA, et al. Identification of functional platelet-activating factor receptors in Raji lymphoblasts. J Immunol. 1989;143(11):3708-13.
Travers, J. B., Li, Q., Kniss, D. A., & Fertel, R. H. (1989). Identification of functional platelet-activating factor receptors in Raji lymphoblasts. Journal of Immunology (Baltimore, Md. : 1950), 143(11), 3708-13.
Travers JB, et al. Identification of Functional Platelet-activating Factor Receptors in Raji Lymphoblasts. J Immunol. 1989 Dec 1;143(11):3708-13. PubMed PMID: 2555416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of functional platelet-activating factor receptors in Raji lymphoblasts. AU - Travers,J B, AU - Li,Q, AU - Kniss,D A, AU - Fertel,R H, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 3708 EP - 13 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 143 IS - 11 N2 - The binding and metabolism of platelet-activating factor (PAF) were characterized in Raji, a human Burkitt's lymphoma-derived cell line. Raji lymphoblasts readily metabolized PAF by deacetylation-reacylation at 37 degrees C, but not at 4 degrees C. Binding studies conducted at 4 degrees C demonstrated specific binding that reached saturation within 80 min. This binding was only partially reversible. Scatchard analysis of PAF binding data revealed a single class of PAF binding sites (17,800 +/- 3,600/cell) with a K of 2.3 +/- 0.3 nM. These high-affinity PAF binding sites were shown to be functional receptors, as 100 pM to 1 microM PAF increased free intracellular calcium in a dose-dependent manner. The dose of PAF necessary to achieve half maximal calcium mobilization response was 6.3 nM, which was in the range of the K for the receptor calculated from the binding studies. The structurally dissimilar PAF receptor antagonists CV-3988 and BN52021 inhibited the PAF-induced calcium changes at doses that competed with PAF binding. These studies provide the first evidence for a functional PAF receptor expressed on a lymphocyte cell line. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2555416/Identification_of_functional_platelet_activating_factor_receptors_in_Raji_lymphoblasts_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2555416 DB - PRIME DP - Unbound Medicine ER -