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OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice.
PLoS One. 2015; 10(1):e116676.Plos

Abstract

Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance.

Authors+Show Affiliations

Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; School of Life Science, Hunan University of Science and Technology, Xiangtan, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; Research Institute of Science and Technology Information, Hunan Academy of Agricultural Sciences, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; State Key laboratory of Main Crop Germplasm Innovation, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25555239

Citation

Zhou, Xiaoyun, et al. "OsGL1-3 Is Involved in Cuticular Wax Biosynthesis and Tolerance to Water Deficit in Rice." PloS One, vol. 10, no. 1, 2015, pp. e116676.
Zhou X, Li L, Xiang J, et al. OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice. PLoS One. 2015;10(1):e116676.
Zhou, X., Li, L., Xiang, J., Gao, G., Xu, F., Liu, A., Zhang, X., Peng, Y., Chen, X., & Wan, X. (2015). OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice. PloS One, 10(1), e116676. https://doi.org/10.1371/journal.pone.0116676
Zhou X, et al. OsGL1-3 Is Involved in Cuticular Wax Biosynthesis and Tolerance to Water Deficit in Rice. PLoS One. 2015;10(1):e116676. PubMed PMID: 25555239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice. AU - Zhou,Xiaoyun, AU - Li,Linzhi, AU - Xiang,Jianhua, AU - Gao,Guofu, AU - Xu,Faxi, AU - Liu,Ailing, AU - Zhang,Xianwen, AU - Peng,Yan, AU - Chen,Xinbo, AU - Wan,Xiangyuan, Y1 - 2015/01/02/ PY - 2014/08/02/received PY - 2014/12/10/accepted PY - 2015/1/3/entrez PY - 2015/1/3/pubmed PY - 2016/1/27/medline SP - e116676 EP - e116676 JF - PloS one JO - PLoS One VL - 10 IS - 1 N2 - Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25555239/OsGL1_3_is_involved_in_cuticular_wax_biosynthesis_and_tolerance_to_water_deficit_in_rice_ L2 - https://dx.plos.org/10.1371/journal.pone.0116676 DB - PRIME DP - Unbound Medicine ER -