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Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS.
Theor Appl Genet. 2015 Mar; 128(3):517-28.TA

Abstract

KEY MESSAGE

Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F1, F2, and F2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.

Authors+Show Affiliations

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25556931

Citation

Shen, X K., et al. "Identification and Genetic Mapping of the Putative Thinopyrum Intermedium-derived Dominant Powdery Mildew Resistance Gene PmL962 On Wheat Chromosome Arm 2BS." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 3, 2015, pp. 517-28.
Shen XK, Ma LX, Zhong SF, et al. Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. Theor Appl Genet. 2015;128(3):517-28.
Shen, X. K., Ma, L. X., Zhong, S. F., Liu, N., Zhang, M., Chen, W. Q., Zhou, Y. L., Li, H. J., Chang, Z. J., Li, X., Bai, G. H., Zhang, H. Y., Tan, F. Q., Ren, Z. L., & Luo, P. G. (2015). Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(3), 517-28. https://doi.org/10.1007/s00122-014-2449-x
Shen XK, et al. Identification and Genetic Mapping of the Putative Thinopyrum Intermedium-derived Dominant Powdery Mildew Resistance Gene PmL962 On Wheat Chromosome Arm 2BS. Theor Appl Genet. 2015;128(3):517-28. PubMed PMID: 25556931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. AU - Shen,X K, AU - Ma,L X, AU - Zhong,S F, AU - Liu,N, AU - Zhang,M, AU - Chen,W Q, AU - Zhou,Y L, AU - Li,H J, AU - Chang,Z J, AU - Li,X, AU - Bai,G H, AU - Zhang,H Y, AU - Tan,F Q, AU - Ren,Z L, AU - Luo,P G, Y1 - 2015/01/04/ PY - 2014/10/21/received PY - 2014/12/17/accepted PY - 2015/1/6/entrez PY - 2015/1/6/pubmed PY - 2015/5/20/medline SP - 517 EP - 28 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 3 N2 - KEY MESSAGE: Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F1, F2, and F2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/25556931/Identification_and_genetic_mapping_of_the_putative_Thinopyrum_intermedium_derived_dominant_powdery_mildew_resistance_gene_PmL962_on_wheat_chromosome_arm_2BS_ DB - PRIME DP - Unbound Medicine ER -