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Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides.
Antonie Van Leeuwenhoek. 2015 Mar; 107(3):799-811.AV

Abstract

Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus Aspergillus, A. niger GNCC 2655 (11.3 U/ml), A. awamori MTCC 2879 (8.2 U/ml), A. niger ATCC 26011 (7.9 U/ml) secreted high titers of inulinase followed by Penicillium sp. NFCCI 2768 (2.6 U/ml) and Penicillium citrinum MTCC 1256 (1.1 U/ml). High sucrase activity was noticed in A. niger GNCC 2613 (113 U/ml) and A. awamori MTCC 2879 (107.8 U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating fructose exclusively from inulin. Five fungi, P. citrinum MTCC 1256, Penicillium rugulosum MTCC 3487, Penicillium sp. NFCCI 2768, A. fumigatus GNCC 1351 and A. niger ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with fructose. High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products.

Authors+Show Affiliations

Department of Applied Microbiology and Biotechnology, Dr. Harisingh Gour University, Sagar, M.P., India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25559021

Citation

Rawat, Hemant Kumar, et al. "Production of Inulinase, Fructosyltransferase and Sucrase From Fungi On Low-value Inulin-rich Substrates and Their Use in Generation of Fructose and Fructo-oligosaccharides." Antonie Van Leeuwenhoek, vol. 107, no. 3, 2015, pp. 799-811.
Rawat HK, Ganaie MA, Kango N. Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides. Antonie Van Leeuwenhoek. 2015;107(3):799-811.
Rawat, H. K., Ganaie, M. A., & Kango, N. (2015). Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides. Antonie Van Leeuwenhoek, 107(3), 799-811. https://doi.org/10.1007/s10482-014-0373-3
Rawat HK, Ganaie MA, Kango N. Production of Inulinase, Fructosyltransferase and Sucrase From Fungi On Low-value Inulin-rich Substrates and Their Use in Generation of Fructose and Fructo-oligosaccharides. Antonie Van Leeuwenhoek. 2015;107(3):799-811. PubMed PMID: 25559021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides. AU - Rawat,Hemant Kumar, AU - Ganaie,Mohd Anis, AU - Kango,Naveen, Y1 - 2015/01/06/ PY - 2014/10/18/received PY - 2014/12/29/accepted PY - 2015/1/7/entrez PY - 2015/1/7/pubmed PY - 2015/12/15/medline SP - 799 EP - 811 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 107 IS - 3 N2 - Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus Aspergillus, A. niger GNCC 2655 (11.3 U/ml), A. awamori MTCC 2879 (8.2 U/ml), A. niger ATCC 26011 (7.9 U/ml) secreted high titers of inulinase followed by Penicillium sp. NFCCI 2768 (2.6 U/ml) and Penicillium citrinum MTCC 1256 (1.1 U/ml). High sucrase activity was noticed in A. niger GNCC 2613 (113 U/ml) and A. awamori MTCC 2879 (107.8 U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating fructose exclusively from inulin. Five fungi, P. citrinum MTCC 1256, Penicillium rugulosum MTCC 3487, Penicillium sp. NFCCI 2768, A. fumigatus GNCC 1351 and A. niger ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with fructose. High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/25559021/Production_of_inulinase_fructosyltransferase_and_sucrase_from_fungi_on_low_value_inulin_rich_substrates_and_their_use_in_generation_of_fructose_and_fructo_oligosaccharides_ L2 - https://doi.org/10.1007/s10482-014-0373-3 DB - PRIME DP - Unbound Medicine ER -