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Genetic markers for western corn rootworm resistance to Bt toxin.
G3 (Bethesda). 2015 Jan 07; 5(3):399-405.G

Abstract

Western corn rootworm (WCR) is a major maize (Zea mays L.) pest leading to annual economic losses of more than 1 billion dollars in the United States. Transgenic maize expressing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely used for the management of WCR. However, cultivation of Bt-expressing maize places intense selection pressure on pest populations to evolve resistance. Instances of resistance to Bt toxins have been reported in WCR. Developing genetic markers for resistance will help in characterizing the extent of existing issues, predicting where future field failures may occur, improving insect resistance management strategies, and in designing and sustainably implementing forthcoming WCR control products. Here, we discover and validate genetic markers in WCR that are associated with resistance to the Cry3Bb1 Bt toxin. A field-derived WCR population known to be resistant to the Cry3Bb1 Bt toxin was used to generate a genetic map and to identify a genomic region associated with Cry3Bb1 resistance. Our results indicate that resistance is inherited in a nearly recessive manner and associated with a single autosomal linkage group. Markers tightly linked with resistance were validated using WCR populations collected from Cry3Bb1 maize fields showing significant WCR damage from across the US Corn Belt. Two markers were found to be correlated with both diet (R2 = 0.14) and plant (R2 = 0.23) bioassays for resistance. These results will assist in assessing resistance risk for different WCR populations, and can be used to improve insect resistance management strategies.

Authors+Show Affiliations

Monsanto Company, Chesterfield, Missouri.Monsanto Company, Creve Coeur, Missouri.Monsanto Company, Chesterfield, Missouri.Monsanto Company, Chesterfield, Missouri.Monsanto Company, Chesterfield, Missouri.Monsanto Company, Creve Coeur, Missouri.Monsanto Company, Chesterfield, Missouri.Monsanto Company, Chesterfield, Missouri.Department of Entomology, Ohio Agricultural Research and Development Center, Wooster, Ohio.Monsanto Company, Chesterfield, Missouri.Monsanto Company, Chesterfield, Missouri.Department of Entomology, Ohio Agricultural Research and Development Center, Wooster, Ohio.Monsanto Company, Creve Coeur, Missouri graham.p.head@monsanto.com barry.s.goldman@monsanto.com.Monsanto Company, Chesterfield, Missouri graham.p.head@monsanto.com barry.s.goldman@monsanto.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25566794

Citation

Flagel, Lex E., et al. "Genetic Markers for Western Corn Rootworm Resistance to Bt Toxin." G3 (Bethesda, Md.), vol. 5, no. 3, 2015, pp. 399-405.
Flagel LE, Swarup S, Chen M, et al. Genetic markers for western corn rootworm resistance to Bt toxin. G3 (Bethesda). 2015;5(3):399-405.
Flagel, L. E., Swarup, S., Chen, M., Bauer, C., Wanjugi, H., Carroll, M., Hill, P., Tuscan, M., Bansal, R., Flannagan, R., Clark, T. L., Michel, A. P., Head, G. P., & Goldman, B. S. (2015). Genetic markers for western corn rootworm resistance to Bt toxin. G3 (Bethesda, Md.), 5(3), 399-405. https://doi.org/10.1534/g3.114.016485
Flagel LE, et al. Genetic Markers for Western Corn Rootworm Resistance to Bt Toxin. G3 (Bethesda). 2015 Jan 7;5(3):399-405. PubMed PMID: 25566794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic markers for western corn rootworm resistance to Bt toxin. AU - Flagel,Lex E, AU - Swarup,Shilpa, AU - Chen,Mao, AU - Bauer,Christopher, AU - Wanjugi,Humphrey, AU - Carroll,Matthew, AU - Hill,Patrick, AU - Tuscan,Meghan, AU - Bansal,Raman, AU - Flannagan,Ronald, AU - Clark,Thomas L, AU - Michel,Andrew P, AU - Head,Graham P, AU - Goldman,Barry S, Y1 - 2015/01/07/ PY - 2015/1/9/entrez PY - 2015/1/9/pubmed PY - 2015/12/15/medline KW - Bacillus thuringiensis KW - Diabrotica virgifera virgifera KW - genetic marker KW - insect resistance KW - western corn rootworm SP - 399 EP - 405 JF - G3 (Bethesda, Md.) JO - G3 (Bethesda) VL - 5 IS - 3 N2 - Western corn rootworm (WCR) is a major maize (Zea mays L.) pest leading to annual economic losses of more than 1 billion dollars in the United States. Transgenic maize expressing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely used for the management of WCR. However, cultivation of Bt-expressing maize places intense selection pressure on pest populations to evolve resistance. Instances of resistance to Bt toxins have been reported in WCR. Developing genetic markers for resistance will help in characterizing the extent of existing issues, predicting where future field failures may occur, improving insect resistance management strategies, and in designing and sustainably implementing forthcoming WCR control products. Here, we discover and validate genetic markers in WCR that are associated with resistance to the Cry3Bb1 Bt toxin. A field-derived WCR population known to be resistant to the Cry3Bb1 Bt toxin was used to generate a genetic map and to identify a genomic region associated with Cry3Bb1 resistance. Our results indicate that resistance is inherited in a nearly recessive manner and associated with a single autosomal linkage group. Markers tightly linked with resistance were validated using WCR populations collected from Cry3Bb1 maize fields showing significant WCR damage from across the US Corn Belt. Two markers were found to be correlated with both diet (R2 = 0.14) and plant (R2 = 0.23) bioassays for resistance. These results will assist in assessing resistance risk for different WCR populations, and can be used to improve insect resistance management strategies. SN - 2160-1836 UR - https://www.unboundmedicine.com/medline/citation/25566794/Genetic_markers_for_western_corn_rootworm_resistance_to_Bt_toxin_ DB - PRIME DP - Unbound Medicine ER -