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Scoparone attenuates RANKL-induced osteoclastic differentiation through controlling reactive oxygen species production and scavenging.
Exp Cell Res. 2015 Feb 15; 331(2):267-77.EC

Abstract

Scoparone, one of the bioactive components of Artemisia capillaris Thunb, has various biological properties including immunosuppressive, hepatoprotective, anti-allergic, anti-inflammatory, and antioxidant effects. This study aims at evaluating the anti-osteoporotic effect of scoparone and its underlying mechanism in vitro. Scoparone demonstrated potent cellular antioxidant capacity. It was also found that scoparone inhibited the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation and suppressed cathepsin K and tartrate-resistant acid phosphatase (TRAP) expression via c-jun N-terminal kinase (JNK)/extracellular signal-regulated kinase (ERK)/p38-mediated c-Fos-nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) signaling pathway. During osteoclast differentiation, the production of general reactive oxygen species (ROS) and superoxide anions was dose-dependently attenuated by scoparone. In addition, scoparone diminished NADPH (nicotinamide adenine dinucleotide phosphate) oxidase 1 (Nox1) expression and activation via the tumor necrosis factor receptor-associated factor 6 (TRAF6)-cSrc-phosphatidylinositol 3-kinase (PI3k) signaling pathway and prevented the disruption of mitochondrial electron transport chain system. Furthermore, scoparone augmented the expression of superoxide dismutase 1 (SOD1) and catalase (CAT). The overall results indicate that the inhibitory effect of scoparone on RANKL-induced osteoclast differentiation is attributed to the suppressive effect on ROS and superoxide anion production by inhibiting Nox1 expression and activation and protecting the mitochondrial electron transport chain system and the scavenging effect of ROS resulting from elevated SOD1 and CAT expression.

Authors+Show Affiliations

Department of Food and Nutrition, Hannam University, Daejeon, Republic of Korea.Department of Food and Nutrition, Hannam University, Daejeon, Republic of Korea. Electronic address: haedong@hnu.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25576385

Citation

Lee, Sang-Hyun, and Hae-Dong Jang. "Scoparone Attenuates RANKL-induced Osteoclastic Differentiation Through Controlling Reactive Oxygen Species Production and Scavenging." Experimental Cell Research, vol. 331, no. 2, 2015, pp. 267-77.
Lee SH, Jang HD. Scoparone attenuates RANKL-induced osteoclastic differentiation through controlling reactive oxygen species production and scavenging. Exp Cell Res. 2015;331(2):267-77.
Lee, S. H., & Jang, H. D. (2015). Scoparone attenuates RANKL-induced osteoclastic differentiation through controlling reactive oxygen species production and scavenging. Experimental Cell Research, 331(2), 267-77. https://doi.org/10.1016/j.yexcr.2014.12.018
Lee SH, Jang HD. Scoparone Attenuates RANKL-induced Osteoclastic Differentiation Through Controlling Reactive Oxygen Species Production and Scavenging. Exp Cell Res. 2015 Feb 15;331(2):267-77. PubMed PMID: 25576385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Scoparone attenuates RANKL-induced osteoclastic differentiation through controlling reactive oxygen species production and scavenging. AU - Lee,Sang-Hyun, AU - Jang,Hae-Dong, Y1 - 2015/01/06/ PY - 2014/07/09/received PY - 2014/12/18/revised PY - 2014/12/26/accepted PY - 2015/1/11/entrez PY - 2015/1/13/pubmed PY - 2015/4/25/medline KW - Mitochondrial electron transport chain system KW - Nox1 KW - Osteoclastic differentiation KW - Phase II antioxidant enzymes KW - ROS production KW - Scoparone SP - 267 EP - 77 JF - Experimental cell research JO - Exp. Cell Res. VL - 331 IS - 2 N2 - Scoparone, one of the bioactive components of Artemisia capillaris Thunb, has various biological properties including immunosuppressive, hepatoprotective, anti-allergic, anti-inflammatory, and antioxidant effects. This study aims at evaluating the anti-osteoporotic effect of scoparone and its underlying mechanism in vitro. Scoparone demonstrated potent cellular antioxidant capacity. It was also found that scoparone inhibited the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation and suppressed cathepsin K and tartrate-resistant acid phosphatase (TRAP) expression via c-jun N-terminal kinase (JNK)/extracellular signal-regulated kinase (ERK)/p38-mediated c-Fos-nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) signaling pathway. During osteoclast differentiation, the production of general reactive oxygen species (ROS) and superoxide anions was dose-dependently attenuated by scoparone. In addition, scoparone diminished NADPH (nicotinamide adenine dinucleotide phosphate) oxidase 1 (Nox1) expression and activation via the tumor necrosis factor receptor-associated factor 6 (TRAF6)-cSrc-phosphatidylinositol 3-kinase (PI3k) signaling pathway and prevented the disruption of mitochondrial electron transport chain system. Furthermore, scoparone augmented the expression of superoxide dismutase 1 (SOD1) and catalase (CAT). The overall results indicate that the inhibitory effect of scoparone on RANKL-induced osteoclast differentiation is attributed to the suppressive effect on ROS and superoxide anion production by inhibiting Nox1 expression and activation and protecting the mitochondrial electron transport chain system and the scavenging effect of ROS resulting from elevated SOD1 and CAT expression. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/25576385/Scoparone_attenuates_RANKL_induced_osteoclastic_differentiation_through_controlling_reactive_oxygen_species_production_and_scavenging_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(14)00559-X DB - PRIME DP - Unbound Medicine ER -