Tags

Type your tag names separated by a space and hit enter

Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain.
J Dent Res. 2015 Mar; 94(3):446-54.JD

Abstract

Thermal and mechanical hypersensitivity in the injured region is a common complication. Although it is well known clinically that thermal and mechanical sensitivity of the oral mucosa is different from that of the skin, the mechanisms underlying injured pain of the oral mucosa remain poorly understood. The transient receptor potential (TRP) vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in primary afferent neurons are known to contribute to pathological pain. Therefore, we investigated whether TRPV1 and/or TRPA1 contribute to thermal and mechanical hypersensitivity following oral mucosa or whisker pad skin incision. Strong heat and mechanical and cold hypersensitivity was caused in the buccal mucosa and whisker pad skin following incisions. On day 3 after the incisions, the number of TRPV1-immunoreactive (IR) and TRPA1-IR trigeminal ganglion (TG) neurons innervating the buccal mucosa and whisker pad skin was significantly increased, and the number of TRPV1/TRPA1-IR TG neurons innervating whisker pad skin, but not the buccal mucosa, was significantly increased. Administration of the TRPV1 antagonist, SB366791, to the incised site produced a significant suppression of heat hyperalgesia in both the buccal mucosa and whisker pad skin, as well as mechanical allodynia in the whisker pad skin. Administration of the TRPA1 antagonist, HC-030031, to the incised site suppressed mechanical allodynia and cold hyperalgesia in both the buccal mucosa and whisker pad skin, as well as heat hyperalgesia in the whisker pad skin. These findings indicate that altered expressions of TRPV1 and TRPA1 in TG neurons are involved in thermal and mechanical hypersensitivity following the buccal mucosa and whisker pad skin incision. Moreover, diverse changes in the number of TRPV1 and TRPA1 coexpressed TG neurons in whisker pad skin-incised rats may contribute to the intracellular interactions of TRPV1 and TRPA1 associated with whisker pad skin incision, whereas TRPV1 and TRPA1 expression in individual TG neurons is involved in buccal mucosa-incised pain.

Authors+Show Affiliations

Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan shinoda.masamichi@nihon-u.ac.jp.Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan.Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan Division of Applied System Neuroscience Advanced Medical Research Center, Nihon University Graduate School of Medical Science, Tokyo, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25576470

Citation

Urata, K, et al. "Involvement of TRPV1 and TRPA1 in Incisional Intraoral and Extraoral Pain." Journal of Dental Research, vol. 94, no. 3, 2015, pp. 446-54.
Urata K, Shinoda M, Honda K, et al. Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. J Dent Res. 2015;94(3):446-54.
Urata, K., Shinoda, M., Honda, K., Lee, J., Maruno, M., Ito, R., Gionhaku, N., & Iwata, K. (2015). Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. Journal of Dental Research, 94(3), 446-54. https://doi.org/10.1177/0022034514565645
Urata K, et al. Involvement of TRPV1 and TRPA1 in Incisional Intraoral and Extraoral Pain. J Dent Res. 2015;94(3):446-54. PubMed PMID: 25576470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. AU - Urata,K, AU - Shinoda,M, AU - Honda,K, AU - Lee,J, AU - Maruno,M, AU - Ito,R, AU - Gionhaku,N, AU - Iwata,K, Y1 - 2015/01/09/ PY - 2015/1/11/entrez PY - 2015/1/13/pubmed PY - 2015/5/30/medline KW - mechanical allodynia KW - mouth mucosa KW - thermal allodynia KW - transient receptor potential channels KW - trigeminal ganglion KW - whiskers SP - 446 EP - 54 JF - Journal of dental research JO - J Dent Res VL - 94 IS - 3 N2 - Thermal and mechanical hypersensitivity in the injured region is a common complication. Although it is well known clinically that thermal and mechanical sensitivity of the oral mucosa is different from that of the skin, the mechanisms underlying injured pain of the oral mucosa remain poorly understood. The transient receptor potential (TRP) vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in primary afferent neurons are known to contribute to pathological pain. Therefore, we investigated whether TRPV1 and/or TRPA1 contribute to thermal and mechanical hypersensitivity following oral mucosa or whisker pad skin incision. Strong heat and mechanical and cold hypersensitivity was caused in the buccal mucosa and whisker pad skin following incisions. On day 3 after the incisions, the number of TRPV1-immunoreactive (IR) and TRPA1-IR trigeminal ganglion (TG) neurons innervating the buccal mucosa and whisker pad skin was significantly increased, and the number of TRPV1/TRPA1-IR TG neurons innervating whisker pad skin, but not the buccal mucosa, was significantly increased. Administration of the TRPV1 antagonist, SB366791, to the incised site produced a significant suppression of heat hyperalgesia in both the buccal mucosa and whisker pad skin, as well as mechanical allodynia in the whisker pad skin. Administration of the TRPA1 antagonist, HC-030031, to the incised site suppressed mechanical allodynia and cold hyperalgesia in both the buccal mucosa and whisker pad skin, as well as heat hyperalgesia in the whisker pad skin. These findings indicate that altered expressions of TRPV1 and TRPA1 in TG neurons are involved in thermal and mechanical hypersensitivity following the buccal mucosa and whisker pad skin incision. Moreover, diverse changes in the number of TRPV1 and TRPA1 coexpressed TG neurons in whisker pad skin-incised rats may contribute to the intracellular interactions of TRPV1 and TRPA1 associated with whisker pad skin incision, whereas TRPV1 and TRPA1 expression in individual TG neurons is involved in buccal mucosa-incised pain. SN - 1544-0591 UR - https://www.unboundmedicine.com/medline/citation/25576470/Involvement_of_TRPV1_and_TRPA1_in_incisional_intraoral_and_extraoral_pain_ DB - PRIME DP - Unbound Medicine ER -