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α integrin cytoplasmic tails can rescue the loss of Rho-family GTPase signaling in the C. elegans somatic gonad.
Mech Dev. 2015 May; 136:111-22.MD

Abstract

Integrin signaling relies on multiple, distinct pathways to impact a diverse set of cell behaviors. The Rho family of GTPases are well-established downstream signaling partners of integrins that regulate cell shape, polarity, and migration. The nematode C. elegans provides a simple in vivo system for studying both integrins and the Rho family. Our previous work showed that the C. elegans α integrin cytoplasmic tails have tissue-specific functions during development. Here, we use chimeric α integrins to show that the cytoplasmic tails can rescue the loss of the Rho family of GTPases in three cell types in the somatic gonad. Knockdown of rho-1 by RNAi causes defects in sheath cell actin organization, ovulation, and vulva morphology. Chimeric α integrin ina-1 with the pat-2 cytoplasmic tail can rescue both actin organization and ovulation after rho-1 RNAi, yet cannot restore vulva morphology. Knockdown of cdc-42 by RNAi causes defects in sheath cell actin organization, ovulation, vulva morphology, and distal tip cell migration. Chimeric α integrin pat-2 with the ina-1 cytoplasmic tail can rescue vulva morphology defects and distal tip cell migration after cdc-42 RNAi, yet cannot restore sheath cell actin organization or ovulation. Disruption of Rac yields the same phenotype in distal tip cells regardless of α integrin cytoplasmic tail composition. Taken together, the cytoplasmic tails of α integrins can bypass signaling from members of the Rho family of GTPases during development.

Authors+Show Affiliations

Christopher Newport University, Newport News, VA 23606, United States. Electronic address: christopher.meighan@cnu.edu.Christopher Newport University, Newport News, VA 23606, United States.Christopher Newport University, Newport News, VA 23606, United States.Christopher Newport University, Newport News, VA 23606, United States.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25576691

Citation

Meighan, Christopher M., et al. "Α Integrin Cytoplasmic Tails Can Rescue the Loss of Rho-family GTPase Signaling in the C. Elegans Somatic Gonad." Mechanisms of Development, vol. 136, 2015, pp. 111-22.
Meighan CM, Kelly VE, Krahe EC, et al. Α integrin cytoplasmic tails can rescue the loss of Rho-family GTPase signaling in the C. elegans somatic gonad. Mech Dev. 2015;136:111-22.
Meighan, C. M., Kelly, V. E., Krahe, E. C., & Gaeta, A. J. (2015). Α integrin cytoplasmic tails can rescue the loss of Rho-family GTPase signaling in the C. elegans somatic gonad. Mechanisms of Development, 136, 111-22. https://doi.org/10.1016/j.mod.2014.12.006
Meighan CM, et al. Α Integrin Cytoplasmic Tails Can Rescue the Loss of Rho-family GTPase Signaling in the C. Elegans Somatic Gonad. Mech Dev. 2015;136:111-22. PubMed PMID: 25576691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - α integrin cytoplasmic tails can rescue the loss of Rho-family GTPase signaling in the C. elegans somatic gonad. AU - Meighan,Christopher M, AU - Kelly,Victoria E, AU - Krahe,Elena C, AU - Gaeta,Adriel J, Y1 - 2015/01/07/ PY - 2014/08/18/received PY - 2014/12/02/revised PY - 2014/12/30/accepted PY - 2015/1/11/entrez PY - 2015/1/13/pubmed PY - 2016/1/7/medline KW - C. elegans KW - Cdc42 KW - Gonadogenesis KW - Integrins KW - Rac KW - Rho SP - 111 EP - 22 JF - Mechanisms of development JO - Mech Dev VL - 136 N2 - Integrin signaling relies on multiple, distinct pathways to impact a diverse set of cell behaviors. The Rho family of GTPases are well-established downstream signaling partners of integrins that regulate cell shape, polarity, and migration. The nematode C. elegans provides a simple in vivo system for studying both integrins and the Rho family. Our previous work showed that the C. elegans α integrin cytoplasmic tails have tissue-specific functions during development. Here, we use chimeric α integrins to show that the cytoplasmic tails can rescue the loss of the Rho family of GTPases in three cell types in the somatic gonad. Knockdown of rho-1 by RNAi causes defects in sheath cell actin organization, ovulation, and vulva morphology. Chimeric α integrin ina-1 with the pat-2 cytoplasmic tail can rescue both actin organization and ovulation after rho-1 RNAi, yet cannot restore vulva morphology. Knockdown of cdc-42 by RNAi causes defects in sheath cell actin organization, ovulation, vulva morphology, and distal tip cell migration. Chimeric α integrin pat-2 with the ina-1 cytoplasmic tail can rescue vulva morphology defects and distal tip cell migration after cdc-42 RNAi, yet cannot restore sheath cell actin organization or ovulation. Disruption of Rac yields the same phenotype in distal tip cells regardless of α integrin cytoplasmic tail composition. Taken together, the cytoplasmic tails of α integrins can bypass signaling from members of the Rho family of GTPases during development. SN - 1872-6356 UR - https://www.unboundmedicine.com/medline/citation/25576691/α_integrin_cytoplasmic_tails_can_rescue_the_loss_of_Rho_family_GTPase_signaling_in_the_C__elegans_somatic_gonad_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0925-4773(14)00092-6 DB - PRIME DP - Unbound Medicine ER -